Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RB214_RS14450 Genome accession   NZ_CP133569
Coordinates   3114078..3115118 (-) Length   346 a.a.
NCBI ID   WP_309167648.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. HL-AS1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3109078..3120118
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RB214_RS14425 (RB214_14425) gshB 3109269..3110219 (+) 951 WP_309167645.1 glutathione synthase -
  RB214_RS14430 (RB214_14430) - 3110376..3110933 (+) 558 WP_309167646.1 YqgE/AlgH family protein -
  RB214_RS14435 (RB214_14435) ruvX 3110971..3111420 (+) 450 WP_002961947.1 Holliday junction resolvase RuvX -
  RB214_RS14440 (RB214_14440) - 3111560..3112888 (+) 1329 WP_375144327.1 IS4 family transposase -
  RB214_RS14445 (RB214_14445) pilU 3112932..3114068 (-) 1137 WP_309167647.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RB214_RS14450 (RB214_14450) pilT 3114078..3115118 (-) 1041 WP_309167648.1 type IV pilus twitching motility protein PilT Machinery gene
  RB214_RS14455 (RB214_14455) - 3115121..3115834 (+) 714 WP_182196817.1 YggS family pyridoxal phosphate-dependent enzyme -
  RB214_RS14460 (RB214_14460) proC 3115852..3116673 (+) 822 WP_002962567.1 pyrroline-5-carboxylate reductase -
  RB214_RS14465 (RB214_14465) - 3116700..3117233 (+) 534 WP_309167649.1 YggT family protein -
  RB214_RS14470 (RB214_14470) - 3117294..3117737 (+) 444 WP_309167650.1 DUF4426 domain-containing protein -
  RB214_RS14475 (RB214_14475) - 3117742..3118341 (+) 600 WP_309167651.1 XTP/dITP diphosphatase -
  RB214_RS14480 (RB214_14480) hemW 3118338..3119474 (+) 1137 WP_309167652.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38093.46 Da        Isoelectric Point: 6.2842

>NTDB_id=874969 RB214_RS14450 WP_309167648.1 3114078..3115118(-) (pilT) [Pseudoalteromonas sp. HL-AS1]
MDITELLAFSVQHKASDLHLSSGVSPMIRVDGDVRRINIPALADKDVNSLVYDIMNDNQRKDYEQNLEVDFSFEVPNLAR
FRVNAFNSNRGPAAVFRTIPSEVLTLDDLGAPDIFKTISDTPRGLVLVTGPTGSGKSTTLAAMVDYINQNKHHHILTIED
PIEFVHDNKLSLINQREVHRDTHSFSNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKAMVRSMLSESLRAVISQTLLKKIGGGRVAAHEIMIAVPAIRNLIREDKIAQMYSSIQTGASVGMQTMDQCL
TNLVNRGIITTAAAKEKAQDKTQFGG

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=874969 RB214_RS14450 WP_309167648.1 3114078..3115118(-) (pilT) [Pseudoalteromonas sp. HL-AS1]
ATGGATATTACCGAATTATTAGCGTTTAGTGTGCAACACAAAGCATCCGATTTACATTTATCGTCGGGTGTTTCACCTAT
GATACGCGTAGACGGTGATGTTCGCCGCATTAACATACCAGCCCTTGCAGACAAAGACGTAAACAGTCTGGTTTACGATA
TTATGAACGATAATCAACGCAAGGACTACGAACAAAACCTCGAAGTGGATTTTTCGTTTGAAGTGCCTAATTTAGCACGT
TTTCGTGTGAATGCGTTTAACTCTAATCGCGGGCCTGCTGCGGTATTTCGTACCATACCAAGTGAAGTGTTAACGCTTGA
TGATTTAGGTGCGCCAGATATTTTTAAAACAATCTCTGATACCCCACGTGGACTCGTGTTAGTAACAGGGCCAACGGGTT
CAGGTAAATCAACAACACTTGCCGCTATGGTCGATTATATAAATCAAAATAAACATCACCATATTCTAACAATAGAAGAT
CCAATCGAGTTTGTTCACGATAATAAATTAAGTTTAATTAACCAACGTGAAGTTCACCGCGATACACATAGCTTTTCAAA
CGCGCTACGTAGTGCATTGCGTGAAGACCCTGATGTAATACTTGTGGGTGAGTTACGTGATTTAGAAACAATTCGTCTTG
CAATGACCGCTGCCGAAACGGGTCACTTAGTGTTTGGCACGCTACATACAACATCTGCACCAAAAACTATTGACCGTATT
ATTGATGTATTCCCAGGTGAAGAAAAAGCTATGGTGCGCTCAATGTTATCTGAATCTTTACGTGCGGTAATTTCACAAAC
ACTGCTTAAAAAAATTGGTGGTGGGCGTGTTGCAGCACACGAAATTATGATTGCAGTTCCTGCTATTCGTAACTTAATTC
GTGAAGATAAAATTGCACAAATGTATTCATCTATTCAAACCGGCGCGTCTGTAGGCATGCAAACAATGGATCAGTGCTTA
ACTAATTTAGTTAATCGCGGGATCATCACTACTGCAGCAGCGAAAGAAAAAGCGCAAGACAAAACACAATTTGGTGGGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

76.744

99.422

0.763
  pilT Acinetobacter nosocomialis M2

76.744

99.422

0.763
  pilT Acinetobacter baumannii D1279779

76.744

99.422

0.763
  pilT Acinetobacter baumannii strain A118

76.744

99.422

0.763
  pilT Pseudomonas aeruginosa PAK

76.163

99.422

0.757
  pilT Acinetobacter baylyi ADP1

75.581

99.422

0.751
  pilT Legionella pneumophila strain Lp02

72.965

99.422

0.725
  pilT Legionella pneumophila strain ERS1305867

72.965

99.422

0.725
  pilT Neisseria gonorrhoeae MS11

70.674

98.555

0.697
  pilT Neisseria meningitidis 8013

70.674

98.555

0.697
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.796

97.977

0.694
  pilT Vibrio cholerae strain A1552

70.796

97.977

0.694
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

99.711

0.526
  pilU Vibrio cholerae strain A1552

42.462

93.931

0.399
  pilU Pseudomonas stutzeri DSM 10701

40.483

95.665

0.387
  pilU Acinetobacter baylyi ADP1

39.118

98.266

0.384