Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RDI66_RS06660 Genome accession   NZ_CP133492
Coordinates   1427610..1428995 (-) Length   461 a.a.
NCBI ID   WP_014391364.1    Uniprot ID   -
Organism   Pasteurella multocida strain PM140     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1422610..1433995
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RDI66_RS06630 (RDI66_06630) - 1422737..1424110 (-) 1374 WP_005722571.1 sodium:alanine symporter family protein -
  RDI66_RS06635 (RDI66_06635) - 1424559..1424828 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  RDI66_RS06640 (RDI66_06640) yacG 1424828..1425031 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  RDI66_RS06645 (RDI66_06645) coaE 1425042..1425662 (-) 621 WP_014391361.1 dephospho-CoA kinase -
  RDI66_RS06650 (RDI66_06650) - 1425677..1426399 (-) 723 WP_041423102.1 A24 family peptidase -
  RDI66_RS06655 (RDI66_06655) - 1426399..1427610 (-) 1212 WP_079157761.1 type II secretion system F family protein -
  RDI66_RS06660 (RDI66_06660) pilB 1427610..1428995 (-) 1386 WP_014391364.1 GspE/PulE family protein Machinery gene
  RDI66_RS06665 (RDI66_06665) pilA 1428995..1429429 (-) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  RDI66_RS06670 (RDI66_06670) ampD 1429589..1430155 (+) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RDI66_RS06675 (RDI66_06675) rppH 1430741..1431337 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  RDI66_RS06680 (RDI66_06680) - 1431340..1432134 (+) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  RDI66_RS06685 (RDI66_06685) lgt 1432144..1432956 (+) 813 WP_014391365.1 prolipoprotein diacylglyceryl transferase -
  RDI66_RS06690 (RDI66_06690) - 1432953..1433804 (+) 852 WP_014391366.1 thymidylate synthase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52255.91 Da        Isoelectric Point: 6.5608

>NTDB_id=874774 RDI66_RS06660 WP_014391364.1 1427610..1428995(-) (pilB) [Pasteurella multocida strain PM140]
MFHENPLCTVSTMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPHFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFTELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCDCRV
ETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=874774 RDI66_RS06660 WP_014391364.1 1427610..1428995(-) (pilB) [Pasteurella multocida strain PM140]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGTACAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGCTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTTAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAATATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTTCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTCACTTTTCGGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTACAGCGAAATAAGCCTATTCATCTCAGTTTTACTGAGTTGGGGATG
ACCGCAGACCAACAACACAGATTCCAACACGCGTTAAGTCAGCCACAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACAGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCTCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTCTTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTGATTGCCGTGTA
GAGACACAATCGGCGTGTCGTTGCTATCAGGGGTATAAAGGAAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-394)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

67.77

98.265

0.666
  pilB Haemophilus influenzae Rd KW20

67.329

98.265

0.662
  pilB Glaesserella parasuis strain SC1401

58.59

98.482

0.577
  pilB Acinetobacter baumannii D1279779

39.757

100

0.425
  pilB Acinetobacter baylyi ADP1

37.876

100

0.41
  pilB Vibrio parahaemolyticus RIMD 2210633

39.293

100

0.41
  pilB Vibrio campbellii strain DS40M4

39.04

100

0.406
  pilB Vibrio cholerae strain A1552

38.272

100

0.403
  pilB Legionella pneumophila strain ERS1305867

37.219

100

0.395