Detailed information NTG0874631

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: RDV59_RS00290
• Genome accession: NZ_CP133476
• Coordinates: 49135..49572 (-)
• Length: 145 a.a.
• NCBI ID: WP_002887014.1
• Uniprot ID: A0A1V0GF29
• Organism: Streptococcus salivarius strain LMG 11489
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 44135..54572

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RDV59_RS00255 (RDV59_00255)	-	44178..44840 (-)	663	WP_002887009.1	CPBP family intramembrane glutamic endopeptidase	-
RDV59_RS00260 (RDV59_00260)	-	44953..45399 (-)	447	WP_037605837.1	hypothetical protein	-
RDV59_RS00265 (RDV59_00265)	-	45473..46129 (-)	657	WP_002887011.1	CPBP family intramembrane glutamic endopeptidase	-
RDV59_RS00270 (RDV59_00270)	-	46141..46338 (-)	198	WP_002885716.1	helix-turn-helix transcriptional regulator	-
RDV59_RS00275 (RDV59_00275)	-	46589..47782 (-)	1194	WP_002885831.1	acetate kinase	-
RDV59_RS00280 (RDV59_00280)	comYH	47839..48795 (-)	957	WP_038676858.1	class I SAM-dependent methyltransferase	Machinery gene
RDV59_RS00285 (RDV59_00285)	comGG	48840..49157 (-)	318	WP_002887013.1	competence type IV pilus minor pilin ComGG	-
RDV59_RS00290 (RDV59_00290)	comYF	49135..49572 (-)	438	WP_002887014.1	competence type IV pilus minor pilin ComGF	Machinery gene
RDV59_RS00295 (RDV59_00295)	comGE	49559..49789 (-)	231	WP_002887015.1	competence type IV pilus minor pilin ComGE	-
RDV59_RS00300 (RDV59_00300)	comYD	49821..50249 (-)	429	WP_254593949.1	competence type IV pilus minor pilin ComGD	Machinery gene
RDV59_RS00305 (RDV59_00305)	comYC	50209..50523 (-)	315	WP_170314557.1	competence type IV pilus major pilin ComGC	Machinery gene
RDV59_RS00310 (RDV59_00310)	comYB	50532..51632 (-)	1101	WP_126405090.1	competence type IV pilus assembly protein ComGB	Machinery gene
RDV59_RS00315 (RDV59_00315)	comGA/cglA/cilD	51514..52455 (-)	942	WP_038676863.1	competence type IV pilus ATPase ComGA	Machinery gene
RDV59_RS00320 (RDV59_00320)	-	52535..52897 (-)	363	WP_002887019.1	DUF1033 family protein	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16155.57 Da
• Isoelectric Point: 8.4658

>NTDB_id=874631 RDV59_RS00290 WP_002887014.1 49135..49572(-) (comYF) [Streptococcus salivarius strain LMG 11489]
MSLNSKVRAFTLLECLVALLVIAGSVQVYQGLTTVLVSNVKQVKQQESQDWLLFVQQMEAELEGCQLVKVEGNKLYVKQD
NQDLAFGLSSATDFRKTNADGRGYQPMLFNVKASTISQNNQIVTIQVTLKNGLQRSFVYAFEKTG

Nucleotide

• Length: 438 bp

>NTDB_id=874631 RDV59_RS00290 WP_002887014.1 49135..49572(-) (comYF) [Streptococcus salivarius strain LMG 11489]
ATGTCTCTCAATAGCAAGGTTCGTGCTTTTACCCTCTTGGAATGTCTAGTGGCCTTGTTGGTGATTGCTGGTTCAGTTCA
AGTTTATCAAGGTCTGACCACAGTATTGGTGAGCAATGTTAAGCAGGTCAAGCAACAAGAAAGCCAAGACTGGCTTCTAT
TCGTTCAACAAATGGAGGCAGAATTAGAAGGTTGTCAGTTGGTAAAGGTAGAGGGGAACAAACTCTATGTCAAGCAGGAT
AATCAAGACTTAGCTTTTGGACTATCAAGTGCTACTGATTTTCGAAAAACAAATGCTGACGGTCGAGGCTACCAACCCAT
GCTTTTTAATGTAAAAGCTAGTACAATTAGCCAAAATAACCAGATAGTCACCATCCAAGTGACTCTTAAAAATGGGCTAC
AAAGGAGTTTTGTTTATGCTTTTGAAAAAACAGGTTAG

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (39-134)

3D structure

• AlphaFold DB: A0A1V0GF29

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	58.156	97.241	0.566
comYF	Streptococcus mutans UA159	57.447	97.241	0.559
comGF/cglF	Streptococcus pneumoniae Rx1	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae D39	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae R6	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae TIGR4	50	97.931	0.49
comGF/cglF	Streptococcus mitis SK321	47.222	99.31	0.469
comGF/cglF	Streptococcus mitis NCTC 12261	47.222	99.31	0.469
comGF	Lactococcus lactis subsp. cremoris KW2	45.324	95.862	0.434