Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   RBU55_RS02335 Genome accession   NZ_CP133110
Coordinates   511992..513044 (+) Length   350 a.a.
NCBI ID   WP_307949866.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aurantiaca strain IRHB3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 506992..518044
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RBU55_RS02325 (RBU55_02325) - 507949..509217 (+) 1269 WP_025808196.1 malic enzyme-like NAD(P)-binding protein -
  RBU55_RS02330 (RBU55_02330) - 509340..511775 (-) 2436 WP_164486060.1 penicillin-binding protein 1A -
  RBU55_RS02335 (RBU55_02335) pilM 511992..513044 (+) 1053 WP_307949866.1 pilus assembly protein PilM Machinery gene
  RBU55_RS02340 (RBU55_02340) - 513044..513610 (+) 567 WP_307949868.1 PilN domain-containing protein -
  RBU55_RS02345 (RBU55_02345) - 513607..514224 (+) 618 WP_307949870.1 type 4a pilus biogenesis protein PilO -
  RBU55_RS02350 (RBU55_02350) - 514221..514748 (+) 528 WP_307949872.1 pilus assembly protein PilP -
  RBU55_RS02355 (RBU55_02355) pilQ 514762..516834 (+) 2073 WP_063427909.1 type IV pilus secretin PilQ Machinery gene
  RBU55_RS02360 (RBU55_02360) aroK 516839..517357 (+) 519 WP_009041824.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37160.62 Da        Isoelectric Point: 5.1262

>NTDB_id=871902 RBU55_RS02335 WP_307949866.1 511992..513044(+) (pilM) [Pseudomonas chlororaphis subsp. aurantiaca strain IRHB3]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGNRFRVEAYGVEPLPANAVVEKSIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLSDDDRESQLKVDADQYIPYPLEEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEM
AGLTARVVDLEACALERSFALAAGSSVARTVALIDIGATLTTLSVVHEGQIIYSREQLFGGHQLTEHIQRRYRLSKEEAG
LAKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQLFQASGPQRQVDSILLAGGSASVPGLAPLIEQRLGLPVLIANPFAGM
SIGGKVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=871902 RBU55_RS02335 WP_307949866.1 511992..513044(+) (pilM) [Pseudomonas chlororaphis subsp. aurantiaca strain IRHB3]
GTGCTAGGACTCTTCAGCAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAGTT
GAGCCGGGTCGGCAACCGCTTTCGGGTCGAGGCCTATGGGGTGGAGCCGTTGCCGGCCAACGCCGTGGTCGAGAAAAGCA
TCGTCGAGCCCGAAGGGGTGGGGCATGCCCTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGAGCGAAGCACGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGACTTTCCGATGATGATCGGGAGAGCCAGCT
CAAGGTCGACGCCGACCAGTACATTCCCTATCCACTGGAGGAGGCGGCCATCGATTTCGACGTCCTCGGCTATTCGGCGC
GTAATCCCGAGCGGGTCGATGTGCTGTTGGCGGCCTGCCGCAAGGAACACATCGAAGTTCGCGAGGCGGCGCTCGAAATG
GCGGGATTGACGGCGCGGGTGGTGGACCTGGAAGCCTGCGCGCTGGAGCGTTCGTTCGCCCTGGCGGCCGGGTCATCGGT
TGCCCGGACCGTGGCGCTGATCGATATCGGCGCCACCCTGACCACCCTGAGCGTTGTGCATGAAGGGCAGATCATCTATT
CCCGCGAGCAGTTGTTTGGCGGGCACCAGTTGACCGAGCACATCCAGCGTCGCTACAGGCTGTCGAAAGAAGAGGCCGGC
CTGGCGAAAAAGCAGGGCGGCCTGCCGGACGACTACGCCAGTGAAGTGCTGCAACCCTTTCAGGAGGCGGTGGTACAGCA
GGTTTCGCGCTCGTTGCAGCTTTTCCAGGCCTCCGGCCCGCAGCGCCAGGTCGATTCCATCCTGTTGGCGGGCGGCAGCG
CCTCGGTTCCGGGGCTTGCGCCGTTGATCGAGCAGCGCCTGGGCTTGCCCGTGCTGATCGCCAACCCCTTCGCTGGCATG
AGCATCGGCGGCAAGGTCGATGCCGCGGCCCTGGCCAGCGAGGCTCCGTCCCTGATGATCGCCTGTGGCCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

54.261

100

0.546
  comM Acinetobacter nosocomialis M2

53.977

100

0.543
  comM Acinetobacter baylyi ADP1

51.42

100

0.517
  pilM Legionella pneumophila strain ERS1305867

44.915

100

0.454