Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   WHU05_RS20000 Genome accession   NZ_CP148363
Coordinates   4059174..4060412 (-) Length   412 a.a.
NCBI ID   WP_000816002.1    Uniprot ID   -
Organism   Escherichia coli strain Z1323HEC0355     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4054174..4065412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHU05_RS19980 (WHU05_19960) dam 4054786..4055622 (-) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -
  WHU05_RS19985 (WHU05_19965) damX 4055729..4057015 (-) 1287 WP_000343172.1 cell division protein DamX -
  WHU05_RS19990 (WHU05_19970) aroB 4057107..4058195 (-) 1089 WP_001356903.1 3-dehydroquinate synthase -
  WHU05_RS19995 (WHU05_19975) aroK 4058252..4058773 (-) 522 WP_000818618.1 shikimate kinase AroK -
  WHU05_RS20000 (WHU05_19980) comE 4059174..4060412 (-) 1239 WP_000816002.1 DNA uptake porin HofQ Machinery gene
  WHU05_RS20005 (WHU05_19985) hofP 4060324..4060728 (-) 405 WP_001356905.1 DNA utilization protein HofP -
  WHU05_RS20010 (WHU05_19990) hofO 4060718..4061158 (-) 441 WP_406874416.1 DNA utilization protein HofO -
  WHU05_RS20015 (WHU05_19995) hofN 4061142..4061681 (-) 540 WP_001069330.1 DNA utilization protein HofN -
  WHU05_RS20020 (WHU05_20000) hofM 4061681..4062460 (-) 780 WP_001296474.1 DNA utilization protein HofM -
  WHU05_RS20025 (WHU05_20005) mrcA 4062580..4065132 (+) 2553 WP_001296475.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44700.19 Da        Isoelectric Point: 6.1173

>NTDB_id=870746 WHU05_RS20000 WP_000816002.1 4059174..4060412(-) (comE) [Escherichia coli strain Z1323HEC0355]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSVAWQNDNIARQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KAALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTALGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=870746 WHU05_RS20000 WP_000816002.1 4059174..4060412(-) (comE) [Escherichia coli strain Z1323HEC0355]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTGGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTTTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATTTAACTGACGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGATTG
ATAACGCGCCAGGAGGGCAACATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGCCAGGAGGCGGA
GCAGACGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAGCGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGTGTGAAATGGACGCTGGCTGATGCGCAACACGCTGGTGGCGTTG
GGCAAGTCACCGCGCTTGGTAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGACGCATCAAC
GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGTTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCTCATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCAGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.615

100

0.398
  comE Haemophilus influenzae 86-028NP

37.385

100

0.396
  pilQ Vibrio campbellii strain DS40M4

38.242

100

0.391
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.53

100

0.376
  pilQ Vibrio cholerae strain A1552

37.53

100

0.376
  comE Glaesserella parasuis strain SC1401

36.429

100

0.371
  pilQ Pseudomonas aeruginosa PAK

34.475

100

0.367