Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RAM23_RS09205 Genome accession   NZ_CP132374
Coordinates   2075646..2076104 (-) Length   152 a.a.
NCBI ID   WP_078475166.1    Uniprot ID   -
Organism   Snodgrassella alvi strain wkB339     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2070646..2081104
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM23_RS09195 (RAM23_09190) waaA 2072659..2073948 (+) 1290 WP_100125854.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  RAM23_RS09200 (RAM23_09195) - 2074133..2075563 (-) 1431 WP_100125853.1 sulfatase-like hydrolase/transferase -
  RAM23_RS09205 (RAM23_09200) pilA 2075646..2076104 (-) 459 WP_078475166.1 pilin Machinery gene
  RAM23_RS09210 (RAM23_09205) - 2076261..2076470 (-) 210 WP_078475165.1 hypothetical protein -
  RAM23_RS09215 (RAM23_09210) - 2076597..2076707 (-) 111 Protein_1781 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  RAM23_RS09220 (RAM23_09215) - 2076866..2077315 (-) 450 WP_078475187.1 pilin -
  RAM23_RS09225 (RAM23_09220) - 2077596..2078399 (-) 804 WP_084561253.1 hypothetical protein -
  RAM23_RS09230 (RAM23_09225) - 2078928..2079971 (+) 1044 Protein_1784 TonB-dependent receptor plug domain-containing protein -
  RAM23_RS09235 (RAM23_09230) - 2079914..2080507 (+) 594 WP_172397424.1 TonB-dependent receptor domain-containing protein -
  RAM23_RS09240 (RAM23_09235) - 2080479..2080988 (+) 510 WP_172397425.1 TonB-dependent receptor domain-containing protein -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 15652.00 Da        Isoelectric Point: 9.6578

>NTDB_id=868751 RAM23_RS09205 WP_078475166.1 2075646..2076104(-) (pilA) [Snodgrassella alvi strain wkB339]
MKAFQKGFTLIELMIVIAIIGILAAIAVPAYSDYIARSQASEASSLAAGLKTQIIDNLQNSNCKSGTNNALDTAIDRQTG
KYGVAEIGGTAATGSISANGATGCTITYTVNTTKVSSSIAGKTLILDVLKNGSLKKNGGTMPDKYVPKAFTI

Nucleotide


Download         Length: 459 bp        

>NTDB_id=868751 RAM23_RS09205 WP_078475166.1 2075646..2076104(-) (pilA) [Snodgrassella alvi strain wkB339]
ATGAAAGCATTCCAAAAAGGTTTTACTTTAATTGAATTGATGATCGTTATTGCAATTATCGGTATTCTTGCTGCAATTGC
TGTACCTGCTTACTCTGATTATATTGCCCGTTCTCAGGCTTCAGAAGCTTCTAGCCTTGCTGCTGGTCTGAAAACTCAGA
TTATTGATAATCTGCAGAATAGTAATTGTAAGTCAGGTACCAATAATGCACTTGATACCGCCATTGACAGACAAACTGGT
AAATATGGTGTAGCAGAAATCGGTGGTACTGCTGCTACCGGCAGTATTTCTGCTAACGGGGCAACTGGCTGTACAATTAC
ATATACAGTAAACACAACCAAAGTATCTTCTTCTATTGCTGGTAAGACTTTGATATTGGATGTATTAAAAAATGGTTCAC
TGAAGAAAAATGGTGGGACTATGCCTGATAAATATGTACCTAAAGCATTCACAATCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-27)

Pilin (bacterial filament) (Pilin)

(36-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter nosocomialis M2

52.381

96.711

0.507
  pilA2 Legionella pneumophila str. Paris

41.497

96.711

0.401
  pilA2 Legionella pneumophila strain ERS1305867

41.497

96.711

0.401
  pilE Neisseria gonorrhoeae MS11

42.143

92.105

0.388
  pilA Pseudomonas aeruginosa PAK

38.926

98.026

0.382