Detailed information NTG0866099

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: B6N23_RS08995
• Genome accession: NZ_CP131913
• Coordinates: 1885735..1886877 (-)
• Length: 380 a.a.
• NCBI ID: WP_305497973.1
• Uniprot ID: -
• Organism: Halomonas alkalicola strain CICC 11012s
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1880735..1891877

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B6N23_RS08985 (B6N23_08990)	tyrS	1883241..1884439 (-)	1199	Protein_1762	tyrosine--tRNA ligase	-
B6N23_RS08990 (B6N23_08995)	-	1884555..1885694 (+)	1140	WP_305497971.1	anhydro-N-acetylmuramic acid kinase	-
B6N23_RS08995 (B6N23_09000)	pilU	1885735..1886877 (-)	1143	WP_305497973.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
B6N23_RS09000 (B6N23_09005)	pilT	1886927..1887961 (-)	1035	WP_119022735.1	type IV pilus twitching motility protein PilT	Machinery gene
B6N23_RS09005 (B6N23_09010)	-	1888028..1888749 (+)	722	Protein_1766	YggS family pyridoxal phosphate-dependent enzyme	-
B6N23_RS09010 (B6N23_09015)	proC	1888820..1889644 (+)	825	WP_305497978.1	pyrroline-5-carboxylate reductase	-
B6N23_RS09015 (B6N23_09020)	-	1889707..1890288 (+)	582	WP_169955853.1	YggT family protein	-
B6N23_RS09020 (B6N23_09025)	-	1890335..1891492 (+)	1158	WP_110070044.1	homoserine O-acetyltransferase	-

Sequence

Protein

• Length: 380 a.a.
• Molecular weight: 42225.55 Da
• Isoelectric Point: 6.1385

>NTDB_id=866099 B6N23_RS08995 WP_305497973.1 1885735..1886877(-) (pilU) [Halomonas alkalicola strain CICC 11012s]
MTAKEWLYQLLDIMVQKEASDLLISVNAPPTLKMAGKLTPLGDQGLSVAQVDELVSASIPEAVMARFQVEREANFALSLR
DKGRFRVSAFQQRNQKAMVIRRIAFEIPHLEELSLPPVLGELANNKRGLVFVVGGTGTGKSTTLASMIQQRNETLGGHII
SVEDPIEYVHPHKKVIINQREVGIDTDSFEVALKNTLRQAPDVILIGEIRTRETMEHALTFAETGHLCLATLHANNANQA
LDRIIHFFPHERHEQIWLDLSLNLRAIVAQQLLPTVDGGRCPAIEIMLKSPLIGDLVRKGEVNEIKNVMSRSRDLGMQTF
DQALYDLFRAGKITEEVALVHADSANDLRMMIKYGDAGSEGLAQAREAASQLSLKRDEDF

Nucleotide

• Length: 1143 bp

>NTDB_id=866099 B6N23_RS08995 WP_305497973.1 1885735..1886877(-) (pilU) [Halomonas alkalicola strain CICC 11012s]
ATGACGGCGAAAGAGTGGCTCTACCAGCTGCTGGATATCATGGTGCAGAAGGAGGCGTCGGATCTGCTGATCTCGGTGAA
TGCACCGCCGACCCTCAAGATGGCCGGCAAGCTGACTCCCCTGGGCGACCAGGGGCTCTCCGTGGCCCAGGTCGACGAGC
TGGTCAGCGCGTCGATCCCGGAGGCGGTGATGGCGCGCTTCCAGGTCGAGCGCGAGGCCAACTTCGCGCTCAGCCTCAGG
GACAAGGGGCGCTTTCGCGTCAGCGCCTTCCAGCAGCGCAACCAGAAGGCGATGGTGATCCGGCGCATCGCCTTCGAGAT
CCCCCACCTGGAGGAGCTGTCGCTGCCGCCGGTGCTGGGCGAGCTGGCCAACAACAAGCGTGGCCTGGTGTTCGTGGTGG
GCGGCACCGGTACCGGCAAGTCGACCACCCTGGCCTCGATGATCCAGCAGCGCAACGAGACCCTGGGGGGCCATATCATC
AGCGTCGAGGATCCCATCGAGTACGTGCATCCGCACAAGAAGGTGATCATCAACCAGCGCGAGGTGGGCATCGACACCGA
CTCCTTCGAGGTCGCGCTGAAGAACACCCTGCGCCAGGCGCCCGACGTGATCCTGATCGGCGAGATCCGCACCCGGGAGA
CCATGGAGCATGCGCTGACCTTCGCCGAGACCGGCCACCTCTGCCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCG
CTGGATCGCATCATCCACTTCTTCCCCCACGAGCGGCACGAGCAGATCTGGCTCGACCTCTCGCTCAACCTGCGCGCCAT
CGTGGCCCAGCAGCTGCTGCCCACGGTGGATGGTGGCCGCTGCCCGGCCATCGAGATCATGCTCAAGTCACCGCTGATCG
GCGATCTGGTACGCAAGGGCGAGGTCAACGAGATCAAGAACGTGATGTCGCGCTCCCGAGACCTGGGGATGCAGACCTTC
GACCAGGCGCTCTACGACCTCTTCCGGGCGGGCAAGATCACCGAGGAGGTGGCGCTGGTGCACGCCGACTCGGCCAACGA
CCTGCGCATGATGATCAAGTACGGTGACGCCGGAAGCGAAGGGCTGGCCCAGGCACGCGAGGCGGCAAGCCAGCTCTCGC
TCAAGCGCGACGAGGACTTCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (66-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	60.858	98.158	0.597
pilU	Vibrio cholerae strain A1552	60.345	91.579	0.553
pilU	Acinetobacter baylyi ADP1	54.891	96.842	0.532
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.416	100	0.434
pilT	Neisseria meningitidis 8013	39.942	90.263	0.361