Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   WCU35_RS03790 Genome accession   NZ_CP147532
Coordinates   795015..796508 (-) Length   497 a.a.
NCBI ID   WP_000249378.1    Uniprot ID   -
Organism   Escherichia coli strain GN03139     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 790015..801508
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WCU35_RS03760 (WCU35_03760) gspK 790809..791786 (-) 978 WP_000633203.1 type II secretion system minor pseudopilin GspK -
  WCU35_RS03765 (WCU35_03765) gspJ 791783..792388 (-) 606 WP_001254778.1 type II secretion system minor pseudopilin GspJ -
  WCU35_RS03770 (WCU35_03770) gspI 792385..792756 (-) 372 WP_000820088.1 type II secretion system minor pseudopilin GspI -
  WCU35_RS03775 (WCU35_03775) gspH 792753..793316 (-) 564 WP_001115130.1 type II secretion system minor pseudopilin GspH -
  WCU35_RS03780 (WCU35_03780) gspG 793320..793775 (-) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  WCU35_RS03785 (WCU35_03785) gspF 793792..795015 (-) 1224 WP_001173429.1 type II secretion system inner membrane protein GspF -
  WCU35_RS03790 (WCU35_03790) pilB/pilB1 795015..796508 (-) 1494 WP_000249378.1 type II secretion system ATPase GspE Machinery gene
  WCU35_RS03795 (WCU35_03795) gspD 796508..798568 (-) 2061 WP_000498848.1 type II secretion system secretin GspD -
  WCU35_RS03800 (WCU35_03800) gspC 798598..799557 (-) 960 WP_000135086.1 type II secretion system protein GspC -
  WCU35_RS03805 (WCU35_03805) gspS2 799575..799985 (-) 411 WP_001324279.1 type II secretion system pilot lipoprotein GspS-beta -
  WCU35_RS03810 (WCU35_03810) pilD 800051..800860 (-) 810 WP_000464637.1 prepilin peptidase PppA Machinery gene

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55196.33 Da        Isoelectric Point: 5.7921

>NTDB_id=859940 WCU35_RS03790 WP_000249378.1 795015..796508(-) (pilB/pilB1) [Escherichia coli strain GN03139]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNASFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLYEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSLELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=859940 WCU35_RS03790 WP_000249378.1 795015..796508(-) (pilB/pilB1) [Escherichia coli strain GN03139]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGTCTTTTACGCTGAGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAGCTGATGGAAGATCTCGGTTCTGCTGAGGACTTTTTTACTCTCGCAGAAGAACTACCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCAATCATCAAACTGATCAACGCCATGTTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACACTATATGAAATGCTGCGT
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATTAAGGTGATGGCACGGCTGGATATCGCCGAAAAACGCGTACC
GCAGGATGGGCGCATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCCGCCTGGGGGG
AACGGGTGGTGCTGCGACTGCTGGACAAAAACCAGGCTCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCTCGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACCGGTTCCGGCAAAAGCACCAC
GCTGTACGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATTGCCGTCCAGGCATCGCTTACAGGGCACCT
GGTCCTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACACGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGATTGCCGCCAGTCCGCGCCTGCC
ACCGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCGAACCGTCACGCTGTACCATCCGCAGGGTTGTCCCGCCTG
TAATCATAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Legionella pneumophila strain ERS1305867

42.184

93.964

0.396
  pilB Vibrio parahaemolyticus RIMD 2210633

48.111

79.879

0.384
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Acinetobacter baumannii D1279779

46.366

80.282

0.372
  pilF Thermus thermophilus HB27

45.522

80.885

0.368
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilB Acinetobacter baylyi ADP1

46.41

78.471

0.364