Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   WAZ86_RS19250 Genome accession   NZ_CP147084
Coordinates   3907038..3908519 (+) Length   493 a.a.
NCBI ID   WP_053270589.1    Uniprot ID   -
Organism   Escherichia coli strain KTa005     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3902038..3913519
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WAZ86_RS19230 (WAZ86_19225) gspB 3902207..3902626 (-) 420 WP_000461450.1 putative general secretion pathway protein GspB -
  WAZ86_RS19235 (WAZ86_19230) gspA 3902628..3904097 (-) 1470 WP_053270590.1 type II secretion system protein GspA -
  WAZ86_RS19240 (WAZ86_19235) gspC 3904277..3905092 (+) 816 WP_021570964.1 type II secretion system protein GspC -
  WAZ86_RS19245 (WAZ86_19240) gspD 3905076..3907028 (+) 1953 WP_021547174.1 type II secretion system secretin GspD -
  WAZ86_RS19250 (WAZ86_19245) pilB 3907038..3908519 (+) 1482 WP_053270589.1 type II secretion system ATPase GspE Machinery gene
  WAZ86_RS19255 (WAZ86_19250) gspF 3908516..3909712 (+) 1197 WP_001109573.1 type II secretion system inner membrane protein GspF -
  WAZ86_RS19260 (WAZ86_19255) gspG 3909722..3910159 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  WAZ86_RS19265 (WAZ86_19260) gspH 3910167..3910676 (+) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  WAZ86_RS19270 (WAZ86_19265) gspI 3910673..3911050 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  WAZ86_RS19275 (WAZ86_19270) gspJ 3911043..3911630 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  WAZ86_RS19280 (WAZ86_19275) gspK 3911623..3912606 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54604.58 Da        Isoelectric Point: 6.2412

>NTDB_id=858756 WAZ86_RS19250 WP_053270589.1 3907038..3908519(+) (pilB) [Escherichia coli strain KTa005]
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKIMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFK
YHQLAVTTIGTPVGCPHCHQSGYQGRIAIHEMMVVTPELRAAIHENVDEQALERLVRQQHKALIKNGLQKVISGDTSWDE
VMRVASATLESEA

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=858756 WAZ86_RS19250 WP_053270589.1 3907038..3908519(+) (pilB) [Escherichia coli strain KTa005]
ATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTAT
TTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGT
CACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATC
GATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC
GGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAA
AAATAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTT
ATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTAT
CGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATA
AAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAGCAGGATTTAGAAAATCTCATTCAGCTT
CCGCACGGTATTATCCTGGTGACAGGGCCGACAGGCTCCGGTAAAAGCACCACGCTCTACGCCATCCTTTCGGCGCTGAA
TACTCCCGGCCGCAATATTCTGACGGTAGAAGATCCCGTGGAATATGAGCTGGAAGGCATTGGGCAAACGCAGGTGAATA
CCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAAATT
CGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAG
TGCATCAGGCGCAGTGACCCGGCTCCGCGACATGGGCGTCGAATCATTCCTGCTTTCGTCTTCCCTGGCAGGGATTATCG
CGCAACGTCTGGTTCGTCGCCTGTGTCCGCAATGCCGACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAA
TATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCTGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCAT
AGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCATGAAAATGTGGATGAACAAGCACTGGAGC
GACTAGTCCGGCAACAACACAAGGCCTTAATCAAAAATGGCCTGCAAAAAGTGATAAGCGGTGACACCTCCTGGGATGAG
GTTATGCGCGTCGCCAGTGCCACGCTGGAGAGCGAAGCATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(114-380)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

46.682

88.641

0.414
  pilB Vibrio cholerae strain A1552

48.441

84.584

0.41
  pilB Acinetobacter baumannii D1279779

45.578

89.452

0.408
  pilB Legionella pneumophila strain ERS1305867

41.372

97.566

0.404
  pilB Vibrio parahaemolyticus RIMD 2210633

46.353

86.207

0.4
  pilB/pilB1 Synechocystis sp. PCC 6803

40.966

96.552

0.396
  pilB Acinetobacter baylyi ADP1

48.338

79.31

0.383
  pilF Thermus thermophilus HB27

45.316

80.122

0.363