Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   WAZ52_RS16565 Genome accession   NZ_CP147038
Coordinates   3346402..3347640 (+) Length   412 a.a.
NCBI ID   WP_000816002.1    Uniprot ID   -
Organism   Escherichia coli strain 21F61     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3341402..3352640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WAZ52_RS16540 (WAZ52_16540) mrcA 3341682..3344234 (-) 2553 WP_001296475.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  WAZ52_RS16545 (WAZ52_16545) hofM 3344354..3345133 (+) 780 WP_001318076.1 DNA utilization protein HofM -
  WAZ52_RS16550 (WAZ52_16550) hofN 3345133..3345672 (+) 540 WP_001069330.1 DNA utilization protein HofN -
  WAZ52_RS16555 (WAZ52_16555) hofO 3345656..3346096 (+) 441 WP_001356906.1 DNA utilization protein HofO -
  WAZ52_RS16560 (WAZ52_16560) hofP 3346086..3346490 (+) 405 WP_001318075.1 DNA utilization protein HofP -
  WAZ52_RS16565 (WAZ52_16565) comE 3346402..3347640 (+) 1239 WP_000816002.1 DNA uptake porin HofQ Machinery gene
  WAZ52_RS16570 (WAZ52_16570) aroK 3348041..3348562 (+) 522 WP_000818618.1 shikimate kinase AroK -
  WAZ52_RS16575 (WAZ52_16575) aroB 3348619..3349707 (+) 1089 WP_000439850.1 3-dehydroquinate synthase -
  WAZ52_RS16580 (WAZ52_16580) damX 3349799..3351085 (+) 1287 WP_000343222.1 cell division protein DamX -
  WAZ52_RS16585 (WAZ52_16585) dam 3351192..3352028 (+) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44700.19 Da        Isoelectric Point: 6.1173

>NTDB_id=857764 WAZ52_RS16565 WP_000816002.1 3346402..3347640(+) (comE) [Escherichia coli strain 21F61]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSVAWQNDNIARQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KAALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTALGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=857764 WAZ52_RS16565 WP_000816002.1 3346402..3347640(+) (comE) [Escherichia coli strain 21F61]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTGGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTTTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATTTAACTGACGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGATTG
ATAACGCGCCAGGAGGGCAACATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGCCAGGAGGCGGA
GCAGACGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAGCGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGTGTGAAATGGACGCTGGCTGATGCGCAACACGCTGGTGGCGTTG
GGCAAGTCACCGCGCTTGGTAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGACGCATCAAC
GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGTTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTTACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGTTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.615

100

0.398
  comE Haemophilus influenzae 86-028NP

37.385

100

0.396
  pilQ Vibrio campbellii strain DS40M4

38.242

100

0.391
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.53

100

0.376
  pilQ Vibrio cholerae strain A1552

37.53

100

0.376
  comE Glaesserella parasuis strain SC1401

36.429

100

0.371
  pilQ Pseudomonas aeruginosa PAK

34.475

100

0.367