Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   V6M96_RS01905 Genome accession   NZ_CP146250
Coordinates   418136..419374 (+) Length   412 a.a.
NCBI ID   WP_000816017.1    Uniprot ID   -
Organism   Escherichia coli strain L26     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 413136..424374
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6M96_RS01880 (V6M96_01880) mrcA 413416..415968 (-) 2553 WP_001301905.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  V6M96_RS01885 (V6M96_01885) hofM 416088..416867 (+) 780 WP_072686287.1 DNA utilization protein HofM -
  V6M96_RS01890 (V6M96_01890) hofN 416867..417406 (+) 540 WP_001069342.1 DNA utilization protein HofN -
  V6M96_RS01895 (V6M96_01895) hofO 417390..417830 (+) 441 WP_001055750.1 DNA utilization protein HofO -
  V6M96_RS01900 (V6M96_01900) hofP 417820..418224 (+) 405 WP_001264140.1 DNA utilization protein HofP -
  V6M96_RS01905 (V6M96_01905) comE 418136..419374 (+) 1239 WP_000816017.1 DNA uptake porin HofQ Machinery gene
  V6M96_RS01910 (V6M96_01910) aroK 419775..420296 (+) 522 WP_000818618.1 shikimate kinase AroK -
  V6M96_RS01915 (V6M96_01915) aroB 420353..421441 (+) 1089 WP_000439848.1 3-dehydroquinate synthase -
  V6M96_RS01920 (V6M96_01920) damX 421533..422819 (+) 1287 WP_000343214.1 cell division protein DamX -
  V6M96_RS01925 (V6M96_01925) dam 422926..423762 (+) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44774.27 Da        Isoelectric Point: 6.1173

>NTDB_id=855034 V6M96_RS01905 WP_000816017.1 418136..419374(+) (comE) [Escherichia coli strain L26]
MKQWIAALLLMLIPSVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSIAWQNDNIARQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=855034 V6M96_RS01905 WP_000816017.1 418136..419374(+) (comE) [Escherichia coli strain L26]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCAGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAACAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGCCAGGAGGGCAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATGACAATATCGCCCGTCAGGAAGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA
AAGCTGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC
AAAACGGCGTTAAGCGCACTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACACGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGTTTGCTGGATCTTGAGCTTTCTGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCTAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAAGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATTCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTGCCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  comE Haemophilus influenzae 86-028NP

36.927

100

0.391
  pilQ Vibrio campbellii strain DS40M4

38.242

100

0.391
  pilQ Vibrio cholerae strain A1552

37.772

100

0.379
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.772

100

0.379
  comE Glaesserella parasuis strain SC1401

35.952

100

0.367
  pilQ Pseudomonas aeruginosa PAK

34.174

100

0.362