NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	QTL84_RS13080	Genome accession	NZ_CP128559
Coordinates	2645592..2646206 (-)	Length	204 a.a.
NCBI ID	WP_014418419.1	Uniprot ID	I2C7Y9
Organism	Bacillus velezensis strain SH-1471
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2640592..2651206

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QTL84_RS13050	rpsT	2640895..2641161 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
QTL84_RS13055	holA	2641178..2642219 (-)	1042	Protein_2518	DNA polymerase III subunit delta	-
QTL84_RS13060	-	2642259..2642410 (-)	152	Protein_2519	hypothetical protein	-
QTL84_RS13065	-	2642451..2642585 (+)	135	WP_003152870.1	YqzM family protein	-
QTL84_RS13070	comEC	2642604..2644955 (-)	2352	WP_014418418.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
QTL84_RS13075	-	2644956..2645525 (-)	570	WP_003152868.1	ComE operon protein 2	-
QTL84_RS13080	comEA	2645592..2646206 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
QTL84_RS13085	comER	2646265..2647086 (+)	822	WP_014418420.1	late competence protein ComER	-
QTL84_RS13090	-	2647155..2647892 (-)	738	WP_014418421.1	class I SAM-dependent methyltransferase	-
QTL84_RS13095	rsfS	2647889..2648245 (-)	357	WP_007408260.1	ribosome silencing factor	-
QTL84_RS13100	yqeK	2648263..2648823 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
QTL84_RS13105	-	2648813..2649382 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
QTL84_RS13110	yhbY	2649393..2649683 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
QTL84_RS13115	aroE	2649677..2650519 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22226.26 Da Isoelectric Point: 5.0355

                                >NTDB_id=848998 QTL84_RS13080 WP_014418419.1 2645592..2646206(-) (comEA) [Bacillus velezensis strain SH-1471]

MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=848998 QTL84_RS13080 WP_014418419.1 2645592..2646206(-) (comEA) [Bacillus velezensis strain SH-1471]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT

GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA

CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	I2C7Y9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363

Detailed information NTG0848998