Detailed information NTG0845100

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: V2197_RS18905
• Genome accession: NZ_CP143901
• Coordinates: 4007014..4007886 (-)
• Length: 290 a.a.
• NCBI ID: WP_023092875.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain PA12_L1_25.22_ST207
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4002014..4012886

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V2197_RS18860 (V2197_18860)	-	4002875..4003045 (-)	171	WP_003094672.1	DUF3094 family protein	-
V2197_RS18865 (V2197_18865)	-	4003071..4003520 (-)	450	WP_003094670.1	MOSC domain-containing protein	-
V2197_RS18870 (V2197_18870)	-	4003517..4004146 (-)	630	WP_003094668.1	DUF1780 domain-containing protein	-
V2197_RS18875 (V2197_18875)	-	4004279..4004704 (+)	426	WP_003094666.1	GNAT family N-acetyltransferase	-
V2197_RS18880 (V2197_18880)	-	4004701..4005171 (+)	471	WP_003094664.1	hypothetical protein	-
V2197_RS18885 (V2197_18885)	-	4005168..4005857 (+)	690	WP_003094662.1	energy-coupling factor ABC transporter permease	-
V2197_RS18890 (V2197_18890)	-	4005963..4006172 (+)	210	WP_003094660.1	hypothetical protein	-
V2197_RS18895 (V2197_18895)	yacG	4006209..4006409 (-)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
V2197_RS18900 (V2197_18900)	coaE	4006406..4007017 (-)	612	WP_003094654.1	dephospho-CoA kinase	-
V2197_RS18905 (V2197_18905)	pilD	4007014..4007886 (-)	873	WP_023092875.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
V2197_RS18910 (V2197_18910)	pilC	4007887..4009104 (-)	1218	WP_033995372.1	type II secretion system F family protein	Machinery gene
V2197_RS18915 (V2197_18915)	pilB	4009108..4010808 (-)	1701	WP_023092873.1	type IV-A pilus assembly ATPase PilB	Machinery gene
V2197_RS18920 (V2197_18920)	pilA/pilAI	4011039..4011503 (+)	465	WP_023092872.1	pilin	Machinery gene

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31861.91 Da
• Isoelectric Point: 7.7068

>NTDB_id=845100 V2197_RS18905 WP_023092875.1 4007014..4007886(-) (pilD) [Pseudomonas aeruginosa strain PA12_L1_25.22_ST207]
MTLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVMV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=845100 V2197_RS18905 WP_023092875.1 4007014..4007886(-) (pilD) [Pseudomonas aeruginosa strain PA12_L1_25.22_ST207]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTTGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTTACCTGGGGCCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGATGGTG
CTGCCGCTGCTCTGGCTGGGTCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterProScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (137-245)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.357	96.552	0.534
pilD	Vibrio campbellii strain DS40M4	52.65	97.586	0.514
pilD	Acinetobacter nosocomialis M2	50.53	97.586	0.493
pilD	Acinetobacter baumannii D1279779	50.53	97.586	0.493
pilD	Neisseria gonorrhoeae MS11	49.64	95.862	0.476