Detailed information NTG0841453

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QO021_RS12280
• Genome accession: NZ_CP127045
• Coordinates: 2575896..2577065 (+)
• Length: 389 a.a.
• NCBI ID: WP_005746411.1
• Uniprot ID: -
• Organism: Pseudomonas amygdali pv. lachrymans strain YM7902
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2570896..2582065

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QO021_RS12260 (QO021_12260)	-	2571353..2572330 (+)	978	WP_007247761.1	IS5 family transposase	-
QO021_RS12265 (QO021_12265)	-	2572537..2573640 (+)	1104	WP_004663854.1	IS5-like element ISPsy19 family transposase	-
QO021_RS12270 (QO021_12270)	pgm	2573776..2575422 (+)	1647	WP_005746410.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
QO021_RS12275 (QO021_12275)	-	2575497..2575772 (-)	276	WP_002553334.1	peptidylprolyl isomerase	-
QO021_RS12280 (QO021_12280)	pilU	2575896..2577065 (+)	1170	WP_005746411.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
QO021_RS12285 (QO021_12285)	-	2577116..2578693 (-)	1578	WP_005746412.1	ABC transporter ATP-binding protein	-
QO021_RS12290 (QO021_12290)	-	2578695..2579717 (-)	1023	WP_005746414.1	ABC transporter permease	-
QO021_RS12295 (QO021_12295)	-	2579717..2580781 (-)	1065	WP_002553338.1	microcin C ABC transporter permease YejB	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42566.73 Da
• Isoelectric Point: 6.0641

>NTDB_id=841453 QO021_RS12280 WP_005746411.1 2575896..2577065(+) (pilU) [Pseudomonas amygdali pv. lachrymans strain YM7902]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETLKPGEVAVIAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGVASLTTPPPAPTGSSTASTAEWGLVDDDAPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=841453 QO021_RS12280 WP_005746411.1 2575896..2577065(+) (pilU) [Pseudomonas amygdali pv. lachrymans strain YM7902]
ATGGATTTTCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTGTATCTTTCCACCGGAGCGCCTCCGTG
CGCCAAGTTCAACGGTGTGCTCAAGCCGCTGGGCAGTGAAACCCTCAAGCCGGGCGAGGTTGCGGTGATCGCCCAAGGGC
TGATGGACGAGGAGCAGAAGCTCGAATTCCTGCGCGAACTGGAGATGAACCTTGCTGTTTCGCTGGCGGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGTAACGAGGTGTCGATCGTCGCGCGGAATATCAAGCTGGATATTCCGCGTTTCGA
GGACCTGTTCCTGCCGCCCGTACTGCTCGATGTGATCATGGAAAAGCACGGGCTGGTGTTGTTCGTCGGTGCCACCGGTT
CGGGCAAATCGACCTCGCTGGCGGCGCTGATCGATTACCGAAATCGCAACGCCAGCGGCCATATCATCACCATTGAAGAC
CCGGTCGAATTCATCCATCGGCACAAGAAGTCCATCGTCAACCAGCGCGAAGTGGGGGTTGATACCCGAAGCTTCCGCGC
GGCGCTGAAGAACACGTTACGTCAGGCTCCGGATGTGATTCTGATCGGCGAAATTCGTGATCGCGAGACCATGGAGCATG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCGATCTCGACCCTGCATGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGAAGAGCGTCGTGCGCAGTTGCTGCATGACCTGGGCAATAACCTCAAGGCGTTCGTGTCCCAGCG
GCTGGTTAGAACCCCGGACGGCAAACGTCGCGCAGCGGTTGAAGTGATGATGGGCACGCCGACCATTCGCGACCTGATTC
AGCGCAATGAACTGACCGAACTCAAGGGCATCATGGAGAAATCCGGCAGCCTCGGTATGCAGACCTTCGACACGGCGTTG
TTCAATCTGGCCGTCGAGGGGGCGATCAGCGAGGAGGAGGCGCTGAAGAATGCCGACTCGCAGAACAACGTTCGTCTGCG
CCTCAAACTGCACAGTGAAGGCGGAGTCGCCAGCCTGACGACGCCGCCCCCGGCCCCGACCGGCAGCAGCACGGCAAGTA
CCGCCGAGTGGGGGCTGGTCGATGACGACGCGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.011	93.059	0.54
pilU	Acinetobacter baylyi ADP1	51.541	91.774	0.473
pilU	Vibrio cholerae strain A1552	51.714	89.974	0.465
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.687	98.458	0.391
pilT	Pseudomonas aeruginosa PAK	41.108	88.175	0.362