Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QO021_RS05085 Genome accession   NZ_CP127045
Coordinates   1100353..1102047 (-) Length   564 a.a.
NCBI ID   WP_005745482.1    Uniprot ID   A0AAD0PUM4
Organism   Pseudomonas amygdali pv. lachrymans strain YM7902     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1095353..1107047
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QO021_RS05045 (QO021_05045) - 1095553..1095660 (-) 108 WP_080164415.1 DUF3094 family protein -
  QO021_RS05050 (QO021_05050) - 1095674..1096207 (-) 534 WP_005745473.1 MOSC domain-containing protein -
  QO021_RS05055 (QO021_05055) - 1096204..1096833 (-) 630 WP_003430735.1 DUF1780 domain-containing protein -
  QO021_RS05060 (QO021_05060) - 1096951..1097232 (+) 282 Protein_994 hypothetical protein -
  QO021_RS05065 (QO021_05065) yacG 1097433..1097642 (-) 210 WP_005745477.1 DNA gyrase inhibitor YacG -
  QO021_RS05070 (QO021_05070) coaE 1097639..1098262 (-) 624 WP_005745478.1 dephospho-CoA kinase -
  QO021_RS05075 (QO021_05075) pilD 1098259..1099131 (-) 873 WP_005745479.1 A24 family peptidase Machinery gene
  QO021_RS05080 (QO021_05080) pilC 1099133..1100350 (-) 1218 WP_005745480.1 type II secretion system F family protein Machinery gene
  QO021_RS05085 (QO021_05085) pilB 1100353..1102047 (-) 1695 WP_005745482.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QO021_RS05090 (QO021_05090) - 1102273..1102659 (+) 387 WP_005745483.1 pilin -
  QO021_RS05095 (QO021_05095) - 1103005..1103982 (+) 978 WP_007247761.1 IS5 family transposase -
  QO021_RS05100 (QO021_05100) - 1104189..1105292 (+) 1104 WP_004663854.1 IS5-like element ISPsy19 family transposase -
  QO021_RS05105 (QO021_05105) - 1105211..1107037 (-) 1827 WP_285230688.1 DUF2075 domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62507.73 Da        Isoelectric Point: 6.2105

>NTDB_id=841446 QO021_RS05085 WP_005745482.1 1100353..1102047(-) (pilB) [Pseudomonas amygdali pv. lachrymans strain YM7902]
MTDAVLTGLAKQLVHAELLTESVAQQAYQQARRDKISLVSYLVQSKLVKSLTLAEMASDQFGVPFMDLASLDKESQPKGL
VSEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTIAIDRFFDSDSGLGNLEDVDLGLD
IEPADGKETSLATQSDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYLEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIQEGFPEEK
IGTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=841446 QO021_RS05085 WP_005745482.1 1100353..1102047(-) (pilB) [Pseudomonas amygdali pv. lachrymans strain YM7902]
ATGACTGATGCTGTCCTCACTGGTTTGGCCAAACAGTTGGTCCACGCCGAGCTACTTACCGAATCTGTTGCGCAGCAAGC
GTATCAGCAGGCGCGCCGTGACAAGATTTCGTTGGTGAGCTATCTGGTCCAGAGCAAATTGGTCAAAAGCCTGACGCTGG
CAGAAATGGCTTCCGACCAGTTTGGCGTGCCATTCATGGACCTTGCCAGCCTGGACAAAGAAAGCCAGCCTAAAGGCCTG
GTGAGTGAAAAGCTGGTTCGACAACATCACGCACTGCCGCTGTGGCGGCGCGGCAATAAACTGTTTATCGGGATTTCGGA
CCCGACCAATCATCAGGCGGTGACTGATATTCAGTTCAGCACGGGCCTGAACACCGAAGCTATTCTGGTCGAAGATGACA
AGCTGACTATTGCTATAGATCGCTTTTTTGACAGTGATAGCGGTCTGGGCAATCTGGAAGATGTAGATCTGGGTCTGGAC
ATCGAACCGGCTGACGGCAAGGAAACCTCCCTTGCTACTCAGAGTGACGCTGACGACGCCCCGGTAGTACGCTTCGTCAA
CAAGATGCTGATGGATGCTATCCGATTAGGGTCATCCGACTTGCACTTCGAACCCTACGAAAAGATCTTCCGCGTGCGGT
TGCGTACTGACGGCATCTTGCATGAAGTCGCCAGACCGCCGATTCATCTGGCAAACCGTATTGCCGCGCGCTTGAAAGTC
ATGGCTAGCCTAGATATCTCGGAACGGCGTAAACCACAGGACGGACGTGTCAAGTTACGTGTGTCCAAAACCAAAGCCAT
CGATTTTCGTATGAATACCTTACCCACGCTATGGGGAGAGAAGATCGTGATGCGGATCCTCGATCCGACCAGTGCGCAGA
TGGGCATTGACGCTCTGGGCTACGAGCCAGAACAAAAAGCGCTTTATCTTGAAGCGCTGAAGCAACCACAAGGCATGATT
CTTGTGACCGGCCCCACTGGCTCGGGTAAAACCGTTTCCCTATACACTGGCCTGAACATTCTCAACACTGTGGACATCAA
CATTTCCACAGCTGAAGACCCGGTAGAGATCAACCTGGAAGGCATCAATCAGGTCAACGTCAACCCGCGTCAGGGACTGG
ACTTTTCTCAGGCATTGCGCGCATTCCTGCGTCAAGATCCCGACGTAATCATGGTCGGCGAGATACGAGACCTGGAAACT
GCCGAAATCGCCATTAAAGCATCACAGACCGGCCACATGGTGTTATCTACTCTGCATACTAACAGTGCCGCAGAAACCCT
CACTCGCTTGCATCATATGGGCGTCGCAGCGTTCAACATCGCTACGGCGATTAACCTCATCATTGCCCAGCGGCTGGCGC
GCAAGCTATGCAGCCATTGCAAGAAAGAGCTCGACATACCCCGGGAAACGCTCATCCAGGAGGGGTTTCCAGAGGAGAAG
ATCGGTACGTTTAAAATCTATGGCCCAGTAGGTTGTGAGCATTGCAACGGCGGCTACAGGGGCAGGGTCGGCATTTACGA
AGTAGTCAAGAAAACACCAGAACTGGAACGCATCATCATGGAAGAAGGCAATTCGCTGGAGATTTCCAGGCAGATGCGCA
AGGACGGCTTCAATGATTTGAGGACCTCCGGACTGTCGAAGGCCATGCAAGGTATTACCAGCCTTGAAGAAGTCAACCGC
GTGACCAAGGATTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.848

100

0.582
  pilB Acinetobacter baylyi ADP1

56.714

100

0.569
  pilB Legionella pneumophila strain ERS1305867

55.221

100

0.553
  pilB Vibrio cholerae strain A1552

51.068

99.645

0.509
  pilB Vibrio parahaemolyticus RIMD 2210633

50.088

100

0.507
  pilB Vibrio campbellii strain DS40M4

49.468

100

0.495
  pilF Neisseria gonorrhoeae MS11

48.221

99.645

0.481
  pilF Thermus thermophilus HB27

39.029

98.582

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.469

93.44

0.369