Detailed information NTG0841245

Bioinformatically predicted

Overview

• Name: pknB
• Type: Regulator
• Locus tag: VVB72_RS09920
• Genome accession: NZ_CP142589
• Coordinates: 2006525..2008408 (-)
• Length: 627 a.a.
• NCBI ID: WP_327063072.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain C20
• Function: modulate the activity of ComDE; modulate the activity of VicKR (predicted from homology) Competence regulation

Genomic Context

Location: 2001525..2013408

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VVB72_RS09890 (VVB72_09890)	-	2001930..2002610 (-)	681	WP_012898438.1	5'-methylthioadenosine/adenosylhomocysteine nucleosidase	-
VVB72_RS09895 (VVB72_09895)	macP	2002643..2002921 (-)	279	WP_003130796.1	cell wall synthase accessory phosphoprotein MacP	-
VVB72_RS09900 (VVB72_09900)	-	2002914..2003498 (-)	585	WP_012898439.1	NUDIX hydrolase	-
VVB72_RS09905 (VVB72_09905)	glmU	2003603..2004979 (-)	1377	WP_003130798.1	bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU	-
VVB72_RS09910 (VVB72_09910)	proC	2005207..2005995 (+)	789	WP_012898440.1	pyrroline-5-carboxylate reductase	-
VVB72_RS09915 (VVB72_09915)	rpsO	2006074..2006343 (-)	270	WP_010906184.1	30S ribosomal protein S15	-
VVB72_RS09920 (VVB72_09920)	pknB	2006525..2008408 (-)	1884	WP_327063072.1	Stk1 family PASTA domain-containing Ser/Thr kinase	Regulator
VVB72_RS09925 (VVB72_09925)	-	2008408..2009184 (-)	777	WP_039116084.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
VVB72_RS09930 (VVB72_09930)	rsmB	2009265..2010539 (-)	1275	WP_014570715.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
VVB72_RS09940 (VVB72_09940)	-	2011742..2012596 (-)	855	WP_039116086.1	GH25 family lysozyme	-
VVB72_RS09945 (VVB72_09945)	-	2012598..2013050 (-)	453	WP_039116087.1	phage holin	-

Sequence

Protein

• Length: 627 a.a.
• Molecular weight: 67929.05 Da
• Isoelectric Point: 4.7426

>NTDB_id=841245 VVB72_RS09920 WP_327063072.1 2006525..2008408(-) (pknB) [Lactococcus lactis subsp. lactis strain C20]
MIQIGKIFADRYRIIKEIGRGGMANVYQGEDTFLGDRLVAIKVLRSNFENDDIAIARFQREAFAMAELSHPNIVGISDVG
EFESQQYIVMEFVDGMTLKQYINQNAPLANDEAIEIITEILSAMDMAHSHGIIHRDLKPQNVLVSSSGTVKVTDFGIAKA
LSETSLTKTNTMFGSVHYLSPEQARGSNATVQSDIYAIGIILFELLTGQIPFDGDSAVAIALKHFQESIPSIINLNPEVP
QALENVVIKATAKDIKNRYTDVEEMMTDVATSTSLDRRGEEKLVFNKEHDETKIMPANLINPYDTKPLIDKKEDNDSQTD
EKAASSEVVNKNKKSKKGLIIGLIILLLVVGGATLAWVVSTPTNVKIPNVTNSTLDQAKSKIKDAKLKVGTVHKQQSSTI
AEGNVIKTDPTSGTTVRSNSSVDIYVSTGNEDIIKMKDFVGEKIDEAMATLLKDYGIDESQVTQTSVPSDSYPAGTIIKQ
SPKKGSSFDTKGSEKITFEVSSGKQVEVPDYKPNGQYMTYSQYQAALKAAGFTNITLDPQATTNQQADGYVYSVYPTVGA
SVDPTQEIVITYSVYTAPSSSSTTSESTTTPETSSSTTSSTSSSTTSQPSTDNNNSSKESSTTSSSS

Nucleotide

• Length: 1884 bp

>NTDB_id=841245 VVB72_RS09920 WP_327063072.1 2006525..2008408(-) (pknB) [Lactococcus lactis subsp. lactis strain C20]
ATGATTCAAATCGGTAAAATCTTTGCCGATCGTTATCGGATTATCAAAGAAATCGGACGTGGCGGAATGGCGAACGTTTA
TCAAGGTGAAGATACTTTTCTTGGTGACCGCTTAGTTGCAATAAAAGTGCTACGTTCAAATTTTGAAAACGATGATATCG
CGATTGCTCGTTTTCAGCGAGAAGCATTCGCCATGGCAGAACTTTCACATCCAAATATCGTAGGTATTTCTGATGTTGGT
GAATTTGAAAGTCAACAATACATTGTCATGGAATTTGTTGACGGCATGACACTTAAACAATACATCAATCAAAATGCACC
TTTAGCCAATGATGAAGCTATTGAAATTATTACTGAAATTTTATCAGCAATGGACATGGCCCATTCACATGGTATTATTC
ACCGGGATTTAAAACCACAAAATGTTTTAGTTTCCAGTAGTGGAACTGTAAAAGTAACGGATTTTGGAATTGCGAAAGCA
CTCTCTGAAACTTCACTTACTAAAACAAATACAATGTTTGGTTCGGTTCATTATTTATCACCAGAGCAAGCGCGTGGTTC
AAATGCAACTGTTCAATCAGATATTTATGCGATTGGAATTATTTTATTTGAACTTTTAACAGGTCAAATTCCATTTGATG
GAGATTCTGCAGTTGCTATCGCTCTAAAACATTTCCAAGAGAGTATTCCAAGCATCATCAATCTCAATCCAGAAGTTCCG
CAAGCTTTGGAAAATGTTGTCATTAAAGCTACAGCGAAAGATATTAAAAATCGCTATACTGATGTAGAAGAAATGATGAC
TGATGTGGCAACTTCAACGAGTCTTGATCGTCGTGGGGAAGAAAAATTAGTTTTCAATAAGGAACATGATGAAACAAAAA
TTATGCCTGCCAATCTAATTAATCCTTATGACACCAAGCCTCTGATTGATAAAAAAGAAGATAATGATTCACAAACAGAT
GAAAAAGCTGCAAGCTCAGAAGTAGTAAATAAAAACAAAAAATCTAAAAAAGGCTTGATTATTGGTCTTATTATCCTTCT
TTTAGTTGTTGGCGGTGCTACTTTGGCTTGGGTTGTCTCAACACCAACTAATGTTAAGATACCTAATGTGACAAACTCGA
CGCTTGACCAAGCAAAAAGCAAAATTAAAGATGCAAAACTAAAAGTGGGAACAGTTCATAAACAACAAAGTTCAACGATT
GCTGAAGGAAATGTTATCAAGACTGATCCAACTAGTGGAACAACCGTGAGATCTAATAGCTCCGTTGATATTTATGTTTC
TACGGGAAATGAAGATATCATCAAAATGAAGGACTTTGTCGGTGAAAAGATTGATGAGGCGATGGCAACATTACTTAAGG
ATTATGGTATTGATGAAAGCCAAGTCACTCAAACTTCCGTTCCTAGTGATTCTTACCCAGCAGGTACAATCATTAAACAA
AGCCCTAAAAAAGGTTCTAGTTTTGATACCAAAGGGAGTGAAAAAATTACCTTTGAAGTTTCAAGTGGAAAACAAGTTGA
AGTTCCGGATTATAAACCGAACGGTCAATATATGACTTATTCACAGTATCAAGCTGCCTTAAAAGCTGCAGGATTCACTA
ATATTACACTAGACCCTCAAGCAACAACGAATCAACAAGCCGACGGTTATGTATATTCTGTTTATCCGACTGTTGGAGCG
TCCGTTGATCCAACTCAAGAAATTGTAATCACTTATAGCGTCTATACTGCTCCAAGTTCAAGTTCAACAACTTCTGAATC
AACAACGACGCCTGAAACTTCGTCAAGCACAACTTCAAGTACTTCAAGTTCTACAACAAGTCAGCCGTCAACAGATAACA
ACAATAGCAGCAAAGAATCTTCAACAACGTCATCAAGTAGTTGA

Domains

Predicted by InterProScan.

• Protein kinase domain (Pkinase) (12-265)

• PASTA domain (PASTA) (506-573)

• PASTA domain (PASTA) (365-428)

• PASTA domain (PASTA) (434-501)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pknB	Streptococcus mutans UA159	48.713	86.762	0.423
stkP/pknB	Streptococcus salivarius strain HSISS4	50.867	82.775	0.421
stkP	Streptococcus pneumoniae TIGR4	47.826	80.702	0.386
stkP	Streptococcus pneumoniae D39	47.628	80.702	0.384
stkP	Streptococcus pneumoniae R6	47.628	80.702	0.384
stkP	Streptococcus pneumoniae Rx1	47.431	80.702	0.383