Detailed information NTG0841134

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QQF68_RS14385
• Genome accession: NZ_CP126992
• Coordinates: 3197789..3198958 (-)
• Length: 389 a.a.
• NCBI ID: WP_044389985.1
• Uniprot ID: A0A3M3YY41
• Organism: Pseudomonas syringae pv. antirrhini str. 126
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3192789..3203958

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QQF68_RS14370 (QQF68_14370)	-	3193945..3195009 (+)	1065	WP_011104338.1	microcin C ABC transporter permease YejB	-
QQF68_RS14375 (QQF68_14375)	-	3195009..3196031 (+)	1023	WP_057417726.1	ABC transporter permease	-
QQF68_RS14380 (QQF68_14380)	-	3196033..3197610 (+)	1578	WP_057417727.1	ABC transporter ATP-binding protein	-
QQF68_RS14385 (QQF68_14385)	pilU	3197789..3198958 (-)	1170	WP_044389985.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
QQF68_RS14390 (QQF68_14390)	-	3199113..3199388 (+)	276	WP_003373489.1	peptidylprolyl isomerase	-
QQF68_RS14395 (QQF68_14395)	pgm	3199464..3201110 (-)	1647	WP_007246443.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
QQF68_RS14400 (QQF68_14400)	-	3201211..3203496 (-)	2286	WP_057417728.1	ATP-dependent DNA helicase	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42668.78 Da
• Isoelectric Point: 5.8663

>NTDB_id=841134 QQF68_RS14385 WP_044389985.1 3197789..3198958(-) (pilU) [Pseudomonas syringae pv. antirrhini str. 126]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLSSETLKPGEVAVVAQGLMDEEQKLEFVRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVKTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVDGAISEEEALKNADSQNNVRLRLKLHSENGVANLTTPPPAPTGSSTASTAEWGLVDDDEPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=841134 QQF68_RS14385 WP_044389985.1 3197789..3198958(-) (pilU) [Pseudomonas syringae pv. antirrhini str. 126]
ATGGATTTTCCGGCGTTGTTGAAGATTCTGGCCAGTCAGGACGGATCTGACCTTTATCTTTCTACCGGAGCACCGCCGTG
TGCCAAGTTCAACGGCGTGCTCAAGCCGCTGAGCAGCGAAACCCTCAAGCCTGGCGAAGTGGCGGTGGTTGCCCAGGGGC
TGATGGACGAGGAGCAGAAGCTCGAGTTTGTCCGGGAACTGGAGATGAACCTCGCGGTGTCATTGGCGGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGCAACGAAGTGTCTATTGTCGCGCGTAACATCAAGCTGGACATTCCGCGCTTTGA
GGACCTTTTCCTGCCGCCGGTATTGCTTGACGTGATCATGGAAAAACACGGTCTGGTGCTGTTTGTCGGTGCCACCGGCT
CGGGTAAATCGACCTCGCTGGCGGCACTGATCGATTACCGTAACCGCAATGCCAGCGGGCACATCATCACCATTGAGGAC
CCGGTCGAATTTATCCATCGGCACAAGAAATCCATCGTCAATCAGCGTGAGGTCGGCGTCGACACCCGCAGCTTTCGCGC
GGCACTGAAAAACACTCTGCGTCAAGCCCCGGATGTGATTCTGATCGGCGAGATCCGCGATCGCGAAACGATGGAGCACG
CGCTGGCATTTGCCGACACCGGCCACTTGGCGATTTCGACCCTGCACGCCAATAACGCCAACCAGGCGCTGGACCGAATC
ATCAACTTTTTCCCGGAAGAGCGGCGTGCGCAGCTGTTGCACGACCTGGGCAACAACCTCAAGGCGTTCGTGTCTCAGCG
GCTGGTAAAGACGCCTGATGGAAAACGGCGCGCGGCGGTGGAAGTGATGATGGGCACGCCGACTATCCGCGACCTGATCC
AGCGTAATGAGCTGACTGAACTCAAGGGCATCATGGAAAAGTCCGGCAGCCTCGGGATGCAGACGTTCGATACCGCGCTG
TTCAACCTGGCCGTCGATGGCGCGATCAGCGAGGAAGAAGCGTTGAAAAATGCCGATTCGCAAAACAACGTTCGTCTGCG
CCTCAAGCTGCACAGCGAGAACGGAGTGGCTAACCTGACCACGCCGCCCCCGGCACCGACCGGCAGCAGCACAGCCAGCA
CCGCCGAGTGGGGCCTGGTCGACGATGACGAGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-248)

3D structure

• AlphaFold DB: A0A3M3YY41

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.072	99.486	0.558
pilU	Acinetobacter baylyi ADP1	52.113	91.26	0.476
pilU	Vibrio cholerae strain A1552	51.429	89.974	0.463
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.164	98.458	0.386
pilT	Pseudomonas aeruginosa PAK	41.399	88.175	0.365