Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   JG741_RS09340 Genome accession   NZ_AP024210
Coordinates   1846573..1847598 (+) Length   341 a.a.
NCBI ID   WP_050574185.1    Uniprot ID   -
Organism   Lactococcus cremoris strain G3-2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1841573..1852598
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JG741_RS09330 (LLG32_19230) - 1841804..1845577 (+) 3774 Protein_1844 PolC-type DNA polymerase III -
  JG741_RS09335 (LLG32_19240) comGA 1845692..1846673 (+) 982 Protein_1845 competence type IV pilus ATPase ComGA -
  JG741_RS09340 (LLG32_19250) comGB 1846573..1847598 (+) 1026 WP_050574185.1 competence type IV pilus assembly protein ComGB Machinery gene
  JG741_RS09345 (LLG32_19260) comGC 1847643..1847993 (+) 351 WP_050574187.1 competence type IV pilus major pilin ComGC Machinery gene
  JG741_RS14345 (LLG32_19270) comGD 1848195..1848383 (+) 189 WP_014573336.1 hypothetical protein Machinery gene
  JG741_RS09355 (LLG32_19280) comGE 1848355..1848651 (+) 297 WP_021164977.1 competence type IV pilus minor pilin ComGE Machinery gene
  JG741_RS09360 (LLG32_19290) comGF 1848635..1849060 (+) 426 WP_014735174.1 competence type IV pilus minor pilin ComGF Machinery gene
  JG741_RS09365 (LLG32_19310) comGG 1849156..1849383 (+) 228 WP_228764408.1 competence protein ComGG Machinery gene
  JG741_RS09370 (LLG32_19320) - 1849463..1849900 (+) 438 WP_011677180.1 zinc-dependent MarR family transcriptional regulator -
  JG741_RS09375 (LLG32_19330) - 1849897..1850739 (+) 843 WP_011677179.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  JG741_RS09380 (LLG32_19340) - 1850918..1851655 (+) 738 WP_011677178.1 metal ABC transporter ATP-binding protein -
  JG741_RS09385 (LLG32_19350) - 1851648..1852457 (+) 810 WP_011677177.1 metal ABC transporter permease -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38393.18 Da        Isoelectric Point: 9.9840

>NTDB_id=84094 JG741_RS09340 WP_050574185.1 1846573..1847598(+) (comGB) [Lactococcus cremoris strain G3-2]
MQTDILRLLRPKGKKLALIKQAKLIQLMGNLLNNGFHLGEVINFLALSKLVEKEFVFKMHQGLSSGNSLSEILDSLSCSK
NVVTQLALVEVHGNLVGTMQLVELHLKKQLKVKKKLIEVATYPVVLLVFLIGIMWGLKNYLLPQIDKGNNFATLLINHLP
LVFFSVGALIVLLTSLSIIIFKHLSALTNFTFLVKVPLVSSFIRLYLTAYFSREWGNLIAQGVELRQIIELMKKQKIRIF
AEVGIKLDLGLKAGQNFEQAVSHFSIFLPELSLMIEYGAIKDKLGLELSVYADECWEQFFSKIDRLMQLIQPLIFIFVAL
MIVLLYAAMSLPIYSNMGSGI

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=84094 JG741_RS09340 WP_050574185.1 1846573..1847598(+) (comGB) [Lactococcus cremoris strain G3-2]
ATGCAAACGGACATCTTACGGCTATTGAGGCCGAAAGGGAAAAAATTAGCATTAATCAAGCAAGCAAAATTGATACAACT
GATGGGGAATCTTTTGAATAATGGTTTCCATTTGGGAGAAGTTATCAATTTTTTAGCTTTATCAAAATTAGTAGAAAAAG
AGTTTGTATTCAAAATGCACCAAGGTCTCAGTTCTGGAAATTCATTATCTGAAATTTTAGATAGTCTCTCTTGTTCTAAA
AATGTTGTGACACAACTTGCTTTGGTAGAAGTGCATGGGAATTTAGTAGGAACCATGCAATTGGTAGAACTGCATTTAAA
AAAGCAGCTTAAGGTCAAAAAGAAATTAATTGAAGTGGCAACTTATCCAGTTGTATTGTTGGTATTTCTCATTGGAATTA
TGTGGGGCTTGAAAAATTATTTATTACCTCAAATTGATAAGGGAAATAATTTTGCTACTTTATTAATTAATCATTTACCG
CTAGTATTTTTTTCTGTTGGTGCTTTAATCGTTTTACTGACAAGTTTATCAATAATAATATTCAAACATCTTTCAGCACT
CACTAATTTTACTTTTTTGGTTAAAGTTCCATTAGTAAGTTCTTTTATCCGCTTGTATTTGACCGCTTATTTCTCCAGAG
AATGGGGAAATTTAATTGCTCAAGGAGTTGAATTGCGACAAATAATAGAATTGATGAAAAAGCAGAAAATTCGCATTTTT
GCGGAAGTTGGAATTAAACTTGATTTGGGTTTGAAGGCTGGTCAGAATTTTGAGCAAGCTGTCAGTCATTTTTCGATTTT
TTTACCAGAACTTTCTTTGATGATTGAGTACGGCGCGATTAAAGATAAATTAGGGTTGGAGCTTTCTGTTTATGCTGATG
AATGTTGGGAACAATTCTTTTCTAAGATAGACCGTCTAATGCAATTAATACAACCCCTTATTTTTATTTTTGTTGCTTTG
ATGATTGTCTTATTGTATGCAGCGATGTCGTTACCCATTTATTCTAATATGGGTTCTGGAATTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(211-332)

Type II secretion system (T2SS), protein F (T2SSF)

(25-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

97.654

100

0.977
  comYB Streptococcus gordonii str. Challis substr. CH1

51.039

98.827

0.504
  comGB/cglB Streptococcus pneumoniae D39

50.299

97.947

0.493
  comGB/cglB Streptococcus pneumoniae R6

50.299

97.947

0.493
  comGB/cglB Streptococcus pneumoniae Rx1

50.299

97.947

0.493
  comGB/cglB Streptococcus pneumoniae TIGR4

50.299

97.947

0.493
  comGB/cglB Streptococcus mitis SK321

50

97.947

0.49
  comGB/cglB Streptococcus mitis NCTC 12261

49.102

97.947

0.481
  comYB Streptococcus mutans UA140

50.955

92.082

0.469
  comYB Streptococcus mutans UA159

50.318

92.082

0.463

Multiple sequence alignment