Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   QN096_RS26060 Genome accession   NZ_CP126342
Coordinates   5630907..5631971 (-) Length   354 a.a.
NCBI ID   WP_044406728.1    Uniprot ID   A0A0D6SQX4
Organism   Pseudomonas otitidis strain TL17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5625907..5636971
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QN096_RS26035 (QN096_26035) aroK 5626483..5627001 (-) 519 WP_074973697.1 shikimate kinase AroK -
  QN096_RS26040 (QN096_26040) pilQ 5627007..5629130 (-) 2124 WP_284547387.1 type IV pilus secretin PilQ Machinery gene
  QN096_RS26045 (QN096_26045) pilP 5629185..5629715 (-) 531 WP_284550262.1 type 4a pilus biogenesis lipoprotein PilP -
  QN096_RS26050 (QN096_26050) pilO 5629715..5630338 (-) 624 WP_284547388.1 type 4a pilus biogenesis protein PilO -
  QN096_RS26055 (QN096_26055) pilN 5630335..5630907 (-) 573 WP_044406731.1 type 4a pilus biogenesis protein PilN -
  QN096_RS26060 (QN096_26060) pilM 5630907..5631971 (-) 1065 WP_044406728.1 pilus assembly protein PilM Machinery gene
  QN096_RS26065 (QN096_26065) - 5632156..5634576 (+) 2421 WP_284550264.1 penicillin-binding protein 1A -
  QN096_RS26070 (QN096_26070) - 5634682..5635950 (-) 1269 WP_284547389.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37701.00 Da        Isoelectric Point: 4.3980

>NTDB_id=837766 QN096_RS26060 WP_044406728.1 5630907..5631971(-) (pilM) [Pseudomonas otitidis strain TL17]
MLGLFNKKANSLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVVAKAKAGSKLAAV
AVAGSAVITKTIEMDAGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGVSARNPERMDVLLAACRRENVEVREAALAL
AGLTAKVVDVEAYALERAYGLLASQLGNGSEDLTVAIVDIGATMTTLSVLNNGRTIYSREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQYNDVDYILLAGGTASIPDLDRLIQQKIGTPTLVANP
FADMALSGKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=837766 QN096_RS26060 WP_044406728.1 5630907..5631971(-) (pilM) [Pseudomonas otitidis strain TL17]
GTGCTAGGGCTCTTCAACAAGAAAGCGAATTCGCTGCTGGGGATCGACATAAGCTCGACCTCGGTCAAGCTCCTCGAATT
GAGTCGCTCGGGTGGCCGCTACAAGGTAGAGGCCTACGCCGTCGAGCCGCTCCCGCCAAATGCTGTCGTCGAAAAGAACA
TTGCTGAGCTGGAAGGTGTTGGCCAGGCACTGTCTCGTGTGGTCGCCAAGGCGAAGGCAGGCTCCAAGCTGGCAGCCGTT
GCTGTGGCGGGTTCAGCCGTCATCACCAAGACGATCGAGATGGATGCGGGGCTGTCCGATGACGAGCTGGAGAACCAGCT
CAAGATCGAAGCTGACCAGTACATTCCTTATCCCCTTGAAGAGGTAGCCATCGATTTCGAGGTTCAGGGCGTTTCTGCAC
GAAACCCCGAGCGAATGGATGTGCTGCTGGCAGCCTGCCGTCGCGAGAACGTCGAAGTACGCGAGGCAGCGCTTGCTCTC
GCCGGGCTCACCGCGAAAGTCGTGGATGTCGAGGCCTATGCGCTTGAGCGCGCCTATGGGCTCCTTGCTTCCCAGCTGGG
GAATGGATCGGAGGACCTGACGGTTGCGATCGTGGACATCGGTGCGACGATGACCACCCTGAGTGTCTTGAACAATGGCC
GCACCATATACAGTCGCGAGCAGTTGTTCGGAGGTCGCCAGTTGACCGAAGAAATTCAGCGCCGCTATGGCCTTTCTGTC
GAGGAAGCGGGCTTGGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAGGTTTTGCAACCGTTCAAAGAGGC
AGTTGTCCAGCAAGTCTCGCGGTCGCTGCAGTTCTTTTTCGCAGCGGGCCAGTACAACGATGTCGATTACATCCTCTTGG
CCGGCGGTACAGCCTCTATTCCCGATCTGGATCGCCTGATTCAGCAGAAGATCGGAACGCCGACGCTGGTCGCAAACCCC
TTCGCCGACATGGCGCTGAGCGGCAAAGTCAACGCAGGTGCGCTGGCAAGTGATGCGCCGGCCTTGATGATCGCTTGTGG
CTTGGCAATGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D6SQX4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

57.062

100

0.571
  comM Acinetobacter nosocomialis M2

57.062

100

0.571
  comM Acinetobacter baylyi ADP1

54.237

100

0.542
  pilM Legionella pneumophila strain ERS1305867

48.023

100

0.48