Detailed information NTG0837743

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QN096_RS01905
• Genome accession: NZ_CP126342
• Coordinates: 382425..383570 (+)
• Length: 381 a.a.
• NCBI ID: WP_044403589.1
• Uniprot ID: A0AB36F620
• Organism: Pseudomonas otitidis strain TL17
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 377425..388570

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QN096_RS01875 (QN096_01875)	-	377514..377810 (-)	297	WP_142012827.1	DUF167 domain-containing protein	-
QN096_RS01880 (QN096_01880)	-	377810..378403 (-)	594	WP_074967194.1	YggT family protein	-
QN096_RS01885 (QN096_01885)	-	378495..379682 (-)	1188	WP_284547668.1	hypothetical protein	-
QN096_RS01890 (QN096_01890)	proC	379685..380503 (-)	819	WP_284547669.1	pyrroline-5-carboxylate reductase	-
QN096_RS01895 (QN096_01895)	-	380521..381213 (-)	693	WP_142012820.1	YggS family pyridoxal phosphate-dependent enzyme	-
QN096_RS01900 (QN096_01900)	pilT	381282..382316 (+)	1035	WP_044403590.1	type IV pilus twitching motility protein PilT	Machinery gene
QN096_RS01905 (QN096_01905)	pilU	382425..383570 (+)	1146	WP_044403589.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
QN096_RS28050	-	383512..384957 (-)	1446	WP_308725816.1	TIGR03915 family putative DNA repair protein	-
QN096_RS01920 (QN096_01920)	-	384957..386168 (-)	1212	WP_069566424.1	putative DNA modification/repair radical SAM protein	-
QN096_RS01925 (QN096_01925)	-	386275..386670 (+)	396	WP_074967204.1	TM2 domain-containing protein	-
QN096_RS01930 (QN096_01930)	-	386729..388000 (-)	1272	WP_284547670.1	dihydroorotase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42485.80 Da
• Isoelectric Point: 6.7727

>NTDB_id=837743 QN096_RS01905 WP_044403589.1 382425..383570(+) (pilU) [Pseudomonas otitidis strain TL17]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKVMPVTKNPMSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEQLKLPEVLKKLAMTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEYIHQHQGCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLVADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYNLYSQGEITYEDALLYADSANDLRLMIKLGSETDGEHLTSISGGLSLEVSEDDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=837743 QN096_RS01905 WP_044403589.1 382425..383570(+) (pilU) [Pseudomonas otitidis strain TL17]
ATGGAATTCGAGAAACTGTTGCGCCTGATGGTCGAGAAGGGCGGTTCGGACCTGTTCATCACGGCCGGTGTGCCGCCGTC
GATGAAGGTCAACGGCAAGGTCATGCCGGTCACCAAGAACCCCATGTCGCCGGAGCAGACCCGGGAAACCGTGCTGGGGG
TGATGAACGAGCAGCAGCGCCGCGACTTCGCCGAGAACCACGAGTGCAACTTCGCCATCAGCGCCCGGGGCATCGGCCGT
TTCCGCGTCAGCGCCTTTTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGACCAACATCCCCACCCTGGA
GCAGCTCAAGCTGCCCGAGGTGCTGAAGAAGCTGGCCATGACCAAGCGCGGCCTGGTGCTCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACGTCCCTGGCTGCGATGATCGGCTACCGCAACAAGAACAGCTCCGGCCACATCATCTCCATCGAAGAC
CCCATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGTGAAGTGGGGATCGACACCGAATCCTTCGAGGT
GGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAGGTGCGGACCCGCGAGACCATGGACCACG
CCGTGGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCTGCCGACCGTCAGCAGCAGGTGTGGATGGACCTGTCCCTCAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCGGATGGCAAGGGGCGCCGCGCGGTGATCGAGGTGCTGATCAATACCCCGCTGGTGGCCGACCTGA
TCCGCAAGGGCGAGGTCCACGAGCTCAAGCCGTTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTACTCCCAGGGCGAGATCACCTACGAAGACGCCCTGCTCTACGCCGACTCGGCGAACGACCTGCGCCT
GATGATCAAGCTCGGTTCCGAGACCGACGGCGAACACCTGACCAGCATCAGTGGCGGGCTGTCCCTGGAAGTCTCGGAAG
ACGATCCGGGCCGTCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	92.126	100	0.921
pilU	Acinetobacter baylyi ADP1	63.866	93.701	0.598
pilU	Vibrio cholerae strain A1552	58.857	91.864	0.541
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.75	88.189	0.386