Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VLW43_RS20415 Genome accession   NZ_CP141736
Coordinates   4145582..4147063 (+) Length   493 a.a.
NCBI ID   WP_001219894.1    Uniprot ID   P45759
Organism   Escherichia coli strain 2023CK-01509     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4140582..4152063
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VLW43_RS20395 (VLW43_20395) gspB 4140751..4141170 (-) 420 WP_000461450.1 putative general secretion pathway protein GspB -
  VLW43_RS20400 (VLW43_20400) gspA 4141172..4142641 (-) 1470 WP_338316272.1 type II secretion system protein GspA -
  VLW43_RS20405 (VLW43_20405) gspC 4142821..4143636 (+) 816 WP_001142708.1 type II secretion system protein GspC -
  VLW43_RS20410 (VLW43_20410) gspD 4143620..4145572 (+) 1953 WP_001326512.1 type II secretion system secretin GspD -
  VLW43_RS20415 (VLW43_20415) pilB 4145582..4147063 (+) 1482 WP_001219894.1 type II secretion system ATPase GspE Machinery gene
  VLW43_RS20420 (VLW43_20420) gspF 4147060..4148256 (+) 1197 WP_001109573.1 type II secretion system inner membrane protein GspF -
  VLW43_RS20425 (VLW43_20425) gspG 4148266..4148703 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  VLW43_RS20430 (VLW43_20430) gspH 4148711..4149220 (+) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  VLW43_RS20435 (VLW43_20435) gspI 4149217..4149594 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  VLW43_RS20440 (VLW43_20440) gspJ 4149587..4150174 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  VLW43_RS20445 (VLW43_20445) gspK 4150167..4151150 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54610.56 Da        Isoelectric Point: 6.2412

>NTDB_id=837308 VLW43_RS20415 WP_001219894.1 4145582..4147063(+) (pilB) [Escherichia coli strain 2023CK-01509]
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFK
YHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHKALIKNGLQKVISGDTSWDE
VMRVASATLESEA

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=837308 VLW43_RS20415 WP_001219894.1 4145582..4147063(+) (pilB) [Escherichia coli strain 2023CK-01509]
ATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTAT
TTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGT
CACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATC
GATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC
GGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAA
AAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTT
ATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTAT
CGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATA
AAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAGCAGGATTTAGAAAATCTCATTCAGCTT
CCGCACGGTATTATCCTGGTGACAGGGCCGACAGGCTCCGGTAAAAGCACCACGCTCTACGCCATCCTTTCGGCGCTGAA
TACTCCCGGCCGCAATATTCTGACGGTAGAAGATCCCGTGGAATATGAGCTGGAAGGCATTGGGCAAACGCAGGTGAATA
CCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCAGATGTCGTCATGGTGGGGGAAATT
CGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAG
TGCATCAGGCGCAGTGACCCGGCTCCGCGACATGGGCGTCGAATCATTCCTGCTTTCGTCTTCCCTGGCAGGGATTATCG
CGCAACGTCTGGTTCGTCGCCTGTGTCCGCAATGCCGACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAA
TATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCTGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCAT
GGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCATGAAAATGTGGATGAACAAGCACTGGAGC
GACTAGTCCGGCAACAACACAAGGCCTTAATCAAAAATGGCCTGCAAAAAGTGATAAGCGGTGACACCTCCTGGGATGAG
GTTATGCGCGTCGCCAGTGCCACGCTGGAGAGCGAAGCATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(114-380)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P45759

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

46.682

88.641

0.414
  pilB Vibrio cholerae strain A1552

48.441

84.584

0.41
  pilB Acinetobacter baumannii D1279779

45.578

89.452

0.408
  pilB Legionella pneumophila strain ERS1305867

41.372

97.566

0.404
  pilB Vibrio parahaemolyticus RIMD 2210633

46.353

86.207

0.4
  pilB/pilB1 Synechocystis sp. PCC 6803

41.176

96.552

0.398
  pilB Acinetobacter baylyi ADP1

48.338

79.31

0.383
  pilF Thermus thermophilus HB27

45.316

80.122

0.363
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.178

79.919

0.361