Detailed information NTG0834649

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: U2S75_RS04430
• Genome accession: NZ_CP140420
• Coordinates: 916890..918008 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain 2023CK-00893
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 911890..923008

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U2S75_RS04400 (U2S75_04400)	-	912719..913567 (+)	849	WP_000336551.1	class II glutamine amidotransferase	-
U2S75_RS04405 (U2S75_04405)	-	913820..914275 (+)	456	WP_000782976.1	bacteriohemerythrin	-
U2S75_RS04410 (U2S75_04410)	-	914428..915495 (+)	1068	WP_029746226.1	membrane protein	-
U2S75_RS04415 (U2S75_04415)	-	915495..915818 (+)	324	WP_000442585.1	RnfH family protein	-
U2S75_RS04420 (U2S75_04420)	-	915873..916271 (-)	399	WP_001170993.1	outer membrane protein assembly factor BamE	-
U2S75_RS04425 (U2S75_04425)	fur	916383..916820 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
U2S75_RS04430 (U2S75_04430)	pilU	916890..918008 (-)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
U2S75_RS04435 (U2S75_04435)	-	918112..919202 (+)	1091	WP_085947913.1	IS4-like element ISAba1 family transposase	-
U2S75_RS04440 (U2S75_04440)	pilT	919225..920262 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
U2S75_RS04445 (U2S75_04445)	-	920389..921081 (+)	693	WP_001108519.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=834649 U2S75_RS04430 WP_000347039.1 916890..918008(-) (pilU) [Acinetobacter baumannii strain 2023CK-00893]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=834649 U2S75_RS04430 WP_000347039.1 916890..918008(-) (pilU) [Acinetobacter baumannii strain 2023CK-00893]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTACAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGAAAGCAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395