Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QNH97_RS18445 Genome accession   NZ_CP125957
Coordinates   4263342..4264457 (-) Length   371 a.a.
NCBI ID   WP_283553293.1    Uniprot ID   -
Organism   Pseudomonas sp. G2-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4258342..4269457
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QNH97_RS18430 (QNH97_18430) - 4258883..4260274 (+) 1392 WP_283553290.1 GntP family permease -
  QNH97_RS18435 (QNH97_18435) - 4260282..4261058 (+) 777 WP_283553291.1 3-hydroxybutyrate dehydrogenase -
  QNH97_RS18440 (QNH97_18440) - 4261235..4263190 (+) 1956 WP_283553292.1 acetoacetate--CoA ligase -
  QNH97_RS18445 (QNH97_18445) pilU 4263342..4264457 (-) 1116 WP_283553293.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QNH97_RS18450 (QNH97_18450) - 4264591..4267128 (+) 2538 WP_283553294.1 PAS domain-containing protein -
  QNH97_RS18455 (QNH97_18455) - 4267174..4267449 (+) 276 WP_003182808.1 peptidylprolyl isomerase -
  QNH97_RS18460 (QNH97_18460) - 4267599..4268516 (-) 918 WP_283553295.1 sugar kinase -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41147.09 Da        Isoelectric Point: 5.8685

>NTDB_id=833660 QNH97_RS18445 WP_283553293.1 4263342..4264457(-) (pilU) [Pseudomonas sp. G2-4]
MEIDPLLRILASQDGSDLYMSTGAPPCARFEGVIKPLGNQAFKVGDIAGLAESLMDAEQRLEFDRELEMNLAISMAGVGR
FRVNIFKQRNDVSMVIRNVKLDIPRFEDLKLPLVLLDTIMQKQGLMLFVGATGSGKSTSLAALIDYRNRNSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALSFADTGHLVISTLHAHNANQALDRV
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTRTGQRRAAVEVMLGSPTVADLIRRNELGELKGIMEKSEELGMQSFDQAL
FNLVVEGAIDEEEALKNADSANNLRLRLKLHAESGAVPPADPAAGEWGLVD

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=833660 QNH97_RS18445 WP_283553293.1 4263342..4264457(-) (pilU) [Pseudomonas sp. G2-4]
ATGGAAATCGATCCTTTGTTGCGAATCCTGGCGAGCCAGGATGGCTCCGACCTCTACATGTCCACCGGCGCGCCGCCGTG
CGCGCGATTCGAGGGCGTGATCAAACCCTTGGGTAACCAGGCATTCAAGGTCGGCGACATTGCAGGGCTCGCCGAGTCTT
TGATGGACGCCGAACAGCGCCTTGAGTTCGATCGGGAACTGGAAATGAACCTGGCGATATCGATGGCCGGTGTCGGGCGG
TTCCGGGTGAATATTTTCAAGCAGCGCAACGACGTGTCCATGGTGATACGCAACGTCAAACTGGACATTCCGCGCTTCGA
AGACCTGAAACTGCCGCTGGTATTGCTGGACACCATTATGCAGAAGCAGGGGCTGATGCTGTTCGTTGGCGCGACGGGGT
CGGGCAAATCGACCTCCCTGGCGGCGTTGATCGACTACCGTAATCGCAACAGCAGCGGCCACATCATCACCATCGAAGAC
CCGGTGGAGTATATCCATCGGCACAAGAAATCCATCATCAACCAGCGGGAAGTCGGTGTCGACACCCGCAGTTTTCATGC
TGCGCTGAAGAACACCCTGCGCCAGGCGCCCGACGTGGTGCTGATCGGTGAGATCCGTGATCGCGAAACCATGGAACATG
CCCTGTCCTTTGCCGACACCGGGCACTTGGTGATTTCCACGCTGCATGCCCATAACGCCAACCAGGCGCTGGATCGGGTG
ATCAACTTCTTCCCGGAAGAGCGCCGGCCGCAGTTGCTCAATGACTTGGGCAATAACCTCAAGGCGTTCGTCTCCCAGCG
CCTGGTGCGCACCCGTACGGGCCAGCGTCGGGCTGCGGTGGAGGTCATGCTGGGATCGCCCACCGTGGCCGATTTGATCC
GGCGCAATGAGCTTGGCGAGCTCAAGGGGATTATGGAAAAGTCTGAGGAGCTGGGCATGCAAAGCTTCGACCAGGCCTTG
TTCAACCTGGTGGTGGAAGGGGCTATCGATGAAGAGGAAGCGCTGAAAAACGCTGACTCGGCGAACAACCTGCGCTTGCG
TTTGAAGTTGCATGCCGAGTCGGGCGCGGTGCCTCCAGCTGACCCAGCGGCGGGGGAGTGGGGGTTGGTGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.338

95.687

0.539
  pilU Acinetobacter baylyi ADP1

51.541

96.226

0.496
  pilU Vibrio cholerae strain A1552

52.286

94.34

0.493
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.527

95.148

0.367
  pilT Pseudomonas stutzeri DSM 10701

38.953

92.722

0.361