Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   U0535_RS16725 Genome accession   NZ_CP139846
Coordinates   2816395..2817633 (-) Length   412 a.a.
NCBI ID   WP_019842344.1    Uniprot ID   -
Organism   Escherichia coli strain Nord58     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2811395..2822633
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0535_RS16705 (U0535_16705) dam 2812007..2812842 (-) 836 Protein_2772 adenine-specific DNA-methyltransferase -
  U0535_RS16710 (U0535_16710) damX 2812949..2814235 (-) 1287 WP_001551753.1 cell division protein DamX -
  U0535_RS16715 (U0535_16715) aroB 2814327..2815415 (-) 1089 WP_000439850.1 3-dehydroquinate synthase -
  U0535_RS16720 (U0535_16720) aroK 2815472..2815993 (-) 522 WP_000818618.1 shikimate kinase AroK -
  U0535_RS16725 (U0535_16725) comE 2816395..2817633 (-) 1239 WP_019842344.1 DNA uptake porin HofQ Machinery gene
  U0535_RS16730 (U0535_16730) hofP 2817545..2817949 (-) 405 WP_001551755.1 DNA utilization protein HofP -
  U0535_RS16735 (U0535_16735) hofO 2817939..2818379 (-) 441 WP_001551756.1 DNA utilization protein HofO -
  U0535_RS16740 (U0535_16740) hofN 2818363..2818902 (-) 540 WP_001069326.1 DNA utilization protein HofN -
  U0535_RS16745 (U0535_16745) hofM 2818902..2819681 (-) 780 WP_001551759.1 DNA utilization protein HofM -
  U0535_RS16750 (U0535_16750) mrcA 2819801..2822353 (+) 2553 WP_322471713.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44694.18 Da        Isoelectric Point: 5.9488

>NTDB_id=831902 U0535_RS16725 WP_019842344.1 2816395..2817633(-) (comE) [Escherichia coli strain Nord58]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSVAWQNDNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=831902 U0535_RS16725 WP_019842344.1 2816395..2817633(-) (comE) [Escherichia coli strain Nord58]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAAGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGTCAGGAGGGCAACATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGTCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACGCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGTTGGCTGATGCGCAACAAGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGACGCATCAAC
GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCTCATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTTACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGTTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae 86-028NP

37.3

100

0.396
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  pilQ Vibrio campbellii strain DS40M4

38.242

100

0.391
  pilQ Vibrio cholerae strain A1552

37.772

100

0.379
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.772

100

0.379
  comE Glaesserella parasuis strain SC1401

35.714

100

0.364
  pilQ Pseudomonas aeruginosa PAK

34.174

100

0.362