Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   U0532_RS14245 Genome accession   NZ_CP139808
Coordinates   2959599..2960837 (+) Length   412 a.a.
NCBI ID   WP_000815986.1    Uniprot ID   -
Organism   Escherichia coli strain FLI21     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2954599..2965837
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0532_RS14220 (U0532_14220) mrcA 2954879..2957431 (-) 2553 WP_001300707.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  U0532_RS14225 (U0532_14225) hofM 2957551..2958330 (+) 780 WP_001295166.1 DNA utilization protein HofM -
  U0532_RS14230 (U0532_14230) hofN 2958330..2958869 (+) 540 WP_058905867.1 DNA utilization protein HofN -
  U0532_RS14235 (U0532_14235) hofO 2958853..2959293 (+) 441 WP_001055760.1 DNA utilization protein HofO -
  U0532_RS14240 (U0532_14240) hofP 2959283..2959687 (+) 405 WP_001264141.1 DNA utilization protein HofP -
  U0532_RS14245 (U0532_14245) comE 2959599..2960837 (+) 1239 WP_000815986.1 DNA uptake porin HofQ Machinery gene
  U0532_RS14250 (U0532_14250) aroK 2961238..2961759 (+) 522 WP_000818618.1 shikimate kinase AroK -
  U0532_RS14255 (U0532_14255) aroB 2961816..2962904 (+) 1089 WP_000439850.1 3-dehydroquinate synthase -
  U0532_RS14260 (U0532_14260) damX 2962996..2964282 (+) 1287 WP_072731225.1 cell division protein DamX -
  U0532_RS14265 (U0532_14265) dam 2964389..2965225 (+) 837 WP_000742143.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44743.26 Da        Isoelectric Point: 6.3183

>NTDB_id=831675 U0532_RS14245 WP_000815986.1 2959599..2960837(+) (comE) [Escherichia coli strain FLI21]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSIAWQNNNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSNE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=831675 U0532_RS14245 WP_000815986.1 2959599..2960837(+) (comE) [Escherichia coli strain FLI21]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGGCAGGAAGGCAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATAACAATATCGCCCGCCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC
AAAACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACACGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGTAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGCTTGCTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGCCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAATGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.327

100

0.393
  comE Haemophilus influenzae 86-028NP

37.097

100

0.391
  pilQ Vibrio campbellii strain DS40M4

38.005

100

0.388
  pilQ Vibrio cholerae strain A1552

37.53

100

0.376
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.53

100

0.376
  comE Glaesserella parasuis strain SC1401

35.714

100

0.364
  pilQ Pseudomonas aeruginosa PAK

34.174

100

0.362