Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SM146_RS02290 Genome accession   NZ_CP139360
Coordinates   490799..492280 (-) Length   493 a.a.
NCBI ID   WP_001377938.1    Uniprot ID   -
Organism   Escherichia coli strain EC147566     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 485799..497280
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM146_RS02255 (SM146_02255) istB 486061..486843 (+) 783 WP_001317493.1 IS21-like element IS100kyp family helper ATPase IstB -
  SM146_RS02260 (SM146_02260) gspK 486910..487695 (-) 786 Protein_448 type II secretion system minor pseudopilin GspK -
  SM146_RS02265 (SM146_02265) gspJ 487688..488275 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  SM146_RS02270 (SM146_02270) gspI 488268..488645 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  SM146_RS02275 (SM146_02275) gspH 488642..489151 (-) 510 WP_001076048.1 type II secretion system minor pseudopilin GspH -
  SM146_RS02280 (SM146_02280) gspG 489159..489596 (-) 438 WP_001202880.1 type II secretion system major pseudopilin GspG -
  SM146_RS02285 (SM146_02285) gspF 489606..490802 (-) 1197 WP_096933757.1 type II secretion system inner membrane protein GspF -
  SM146_RS02290 (SM146_02290) pilB 490799..492280 (-) 1482 WP_001377938.1 type II secretion system ATPase GspE Machinery gene
  SM146_RS02295 (SM146_02295) gspD 492290..494242 (-) 1953 WP_001393111.1 type II secretion system secretin GspD -
  SM146_RS02300 (SM146_02300) gspC 494226..495041 (-) 816 WP_001142703.1 type II secretion system protein GspC -
  SM146_RS02305 (SM146_02305) gspA 495221..496690 (+) 1470 WP_000107576.1 type II secretion system protein GspA -
  SM146_RS02310 (SM146_02310) gspB 496692..497111 (+) 420 WP_000461449.1 putative general secretion pathway protein GspB -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54580.49 Da        Isoelectric Point: 6.0991

>NTDB_id=830224 SM146_RS02290 WP_001377938.1 490799..492280(-) (pilB) [Escherichia coli strain EC147566]
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVNLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFI
YHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHNALIKNGLQKVISGDTSWDE
VMRVASATLESEA

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=830224 SM146_RS02290 WP_001377938.1 490799..492280(-) (pilB) [Escherichia coli strain EC147566]
ATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTAT
TTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGT
CACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATC
GATCAATCCGTCAATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC
GGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAA
AAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTT
ATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTAT
CGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATA
AAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAGCAGGATTTAGAAAATCTCATTCAGCTT
CCGCACGGTATTATCCTGGTGACAGGGCCGACAGGCTCCGGTAAAAGCACCACGCTCTACGCCATCCTTTCGGCGCTGAA
TACTCCCGGCCGCAATATTCTGACGGTAGAAGATCCCGTGGAATATGAGCTGGAAGGCATTGGGCAAACGCAGGTGAATA
CCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAAATT
CGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAG
TGCATCAGGCGCAGTGACCCGGCTCCGCGACATGGGCGTCGAATCATTCCTGCTTTCGTCTTCCCTGGCAGGGATTATCG
CGCAACGTCTGGTTCGTCGCCTGTGTCCGCAATGCCGACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTATA
TATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCTGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCAT
GGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCATGAAAATGTGGATGAACAAGCACTGGAGC
GACTAGTCCGGCAACAACACAATGCCTTAATCAAAAATGGCCTGCAAAAAGTGATAAGCGGTGACACCTCCTGGGATGAG
GTTATGCGCGTCGCCAGTGCCACGCTGGAGAGCGAAGCATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(114-380)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

46.364

89.249

0.414
  pilB Vibrio cholerae strain A1552

48.681

84.584

0.412
  pilB Acinetobacter baumannii D1279779

46.014

89.047

0.41
  pilB Legionella pneumophila strain ERS1305867

40.956

97.566

0.4
  pilB Vibrio parahaemolyticus RIMD 2210633

48.259

81.542

0.394
  pilB/pilB1 Synechocystis sp. PCC 6803

40.756

96.552

0.394
  pilB Acinetobacter baylyi ADP1

47.487

80.73

0.383
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.862

80.933

0.363
  pilF Thermus thermophilus HB27

45.063

80.122

0.361