Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   QI003_RS05745 Genome accession   NZ_CP124601
Coordinates   1145554..1149054 (+) Length   1166 a.a.
NCBI ID   WP_282374152.1    Uniprot ID   -
Organism   Bacillus stercoris strain BS21     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1140554..1154054
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QI003_RS05725 (QI003_05725) - 1141406..1142608 (-) 1203 WP_014663454.1 MFS transporter -
  QI003_RS05730 (QI003_05730) - 1142722..1144449 (+) 1728 WP_282374151.1 SgrR family transcriptional regulator -
  QI003_RS05735 (QI003_05735) - 1144485..1144814 (-) 330 WP_014663456.1 four-helix bundle copper-binding protein -
  QI003_RS05740 (QI003_05740) - 1144933..1145370 (-) 438 WP_040083516.1 ferritin family protein -
  QI003_RS05745 (QI003_05745) addB 1145554..1149054 (+) 3501 WP_282374152.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  QI003_RS05750 (QI003_05750) addA 1149041..1152745 (+) 3705 WP_282374153.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  QI003_RS05755 (QI003_05755) sbcD 1152817..1153992 (+) 1176 WP_069839054.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134695.59 Da        Isoelectric Point: 5.4728

>NTDB_id=826596 QI003_RS05745 WP_282374152.1 1145554..1149054(+) (addB) [Bacillus stercoris strain BS21]
MGAEFLVGRSGSGKTKLIINSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDMGGMIRAQVFSFSRLAWRVLQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKNGFTAQVERMLTEFKRYCLEPEDIRRMAESGTASEYRGERVLSE
KLHDLSILYQQMEKSLADQYLHSEDYLTLLAEHIPLAEDVKGAHIYVDGFYQFTPQEFRVLEQLMVHAEHITFSFTADKP
SYEREPHELELFRMTGKTYYRLHQKAKELNLDITYKELSGTERHTQTPELAHLEAQYEVRPAVPYAEKQEALTVMQAANR
RAELEGIAREIHALVREKGYRYKDVAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLIEFIRSSLDVLKGNWRY
EAVFRCVKTELLFPLNEPRAKVREQVDQLENYCIAYGIKGDRWTKGDRFQYRRFVSLDDDFAQTDQEIEMENMLNDTRDW
IVPPLFQLQKRMKKAKTVQEKAEALYRYLEETEVPLKLDQERQRAEEDGRIIEAQQHQQAWDAVIQLLEEFVEMMGDDEI
SLDLFQQMIEAGAESLTFSLIPPALDQVFVGNMDLSRMYGTSCTFVLGANDGVLPARPDENGVLSDDDREWLKTIGVELS
SGGRERLLDEHFLIYMAFSSPSDRLYVSYPIADAEGKTLLPSIVVKRLEELFPHHKERLLTNEPEQVSDEEQLKYVVNQS
VAQSFTASQLRLWTREYDISDVWWSTYNVLMSEPDRLQSKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLDWRDLTKEQCELFSYDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFQLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMRATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEVVRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFDLLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCAFKSVCQFDESLEENEYRPLKAEKDKTILEWIKKEADGNEHS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=826596 QI003_RS05745 WP_282374152.1 1145554..1149054(+) (addB) [Bacillus stercoris strain BS21]
TTGGGAGCAGAGTTTTTAGTAGGCAGGTCCGGGAGTGGAAAAACGAAGCTGATCATCAACAGCATTCAGGATGAATTACG
CCGGGCTCCATTCGGGAAGCCAATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCAGATATGGGCGGGATGATACGCGCTCAAGTGTTCAGCTTTTCACGATTGGCCTGGCGCGTCCTTCAGCATACGGGA
GGAATGAGCAGGCCGTTTCTGACAAGCACCGGCGTACAAATGCTCCTGCGGAAGCTCATTGAGGAGCATAAACAGGAGTT
CAAAGTCTATCAAAAAGCGAGTGACAAAAACGGGTTTACCGCACAGGTTGAGCGGATGCTGACAGAGTTTAAGCGGTATT
GTCTGGAACCGGAGGATATCCGCCGGATGGCGGAAAGCGGAACGGCTTCCGAATATCGCGGAGAACGTGTGTTATCTGAA
AAGCTCCATGACTTATCGATTCTGTATCAGCAAATGGAAAAAAGCCTCGCAGATCAATATCTTCACTCTGAGGATTATTT
GACATTGCTGGCAGAGCATATCCCGCTTGCGGAAGATGTAAAAGGCGCGCATATCTATGTGGATGGCTTTTATCAGTTTA
CACCGCAGGAATTCAGGGTGCTGGAGCAGCTTATGGTTCATGCAGAGCACATCACGTTTTCGTTCACAGCGGACAAGCCG
TCATATGAGCGGGAGCCGCATGAACTGGAATTGTTCAGAATGACGGGGAAAACCTATTACCGCCTGCATCAGAAGGCGAA
GGAACTGAATTTAGACATTACGTATAAAGAGCTGAGTGGGACTGAGCGGCATACACAGACACCCGAATTGGCCCATCTAG
AGGCGCAGTATGAAGTGCGTCCGGCCGTTCCTTACGCAGAAAAACAAGAAGCACTGACTGTAATGCAGGCCGCGAACAGA
CGAGCTGAGCTGGAAGGTATTGCCCGGGAAATTCACGCCCTCGTCAGAGAGAAAGGATATCGCTATAAGGATGTAGCAAT
TCTTGCGCGCCAGCCGGAAGACTACAAGGATATGGTGAAGGAAGTTTTCGCAGATTACGAGATTCCTTATTTCATTGACG
GAAAAGCATCTATGCTGAACCATCCGTTAATCGAATTTATCCGGTCAAGCCTTGATGTTCTGAAAGGGAATTGGCGTTAT
GAAGCGGTGTTTCGCTGTGTGAAAACCGAACTGCTATTCCCGCTCAATGAACCGAGGGCCAAAGTGAGAGAACAGGTCGA
TCAGCTCGAAAATTACTGTATCGCTTATGGGATCAAAGGTGACCGCTGGACAAAGGGCGACCGTTTCCAATATAGGCGCT
TTGTGTCGCTGGATGATGATTTTGCGCAGACCGATCAGGAAATCGAAATGGAAAACATGTTGAATGACACCCGCGATTGG
ATTGTTCCGCCGCTTTTTCAGCTTCAAAAACGAATGAAAAAAGCAAAGACGGTACAGGAGAAGGCGGAGGCGCTCTATCG
CTATTTAGAAGAGACGGAGGTGCCGCTGAAGCTGGATCAGGAAAGGCAGCGTGCTGAGGAAGACGGCAGAATCATTGAAG
CACAGCAGCATCAGCAGGCGTGGGACGCAGTCATTCAGCTGCTTGAGGAGTTTGTTGAAATGATGGGAGATGATGAGATT
TCTCTTGATTTGTTTCAGCAAATGATAGAAGCCGGCGCGGAGTCGCTTACATTTTCGCTTATTCCGCCTGCGCTTGACCA
GGTGTTTGTCGGCAATATGGATTTGTCCAGAATGTATGGCACATCCTGTACCTTTGTGCTCGGGGCAAACGACGGCGTTC
TGCCGGCACGCCCTGATGAAAACGGGGTTCTGTCGGATGATGACCGGGAATGGCTGAAAACGATCGGGGTTGAGCTTTCC
TCAGGCGGACGGGAGCGTTTGCTTGATGAGCATTTCCTCATCTACATGGCATTTTCAAGTCCGTCTGACCGGCTTTATGT
ATCGTATCCGATTGCAGATGCGGAAGGAAAAACGCTTTTGCCGTCGATTGTCGTTAAACGGCTGGAAGAACTGTTTCCGC
ATCATAAGGAGCGCCTGTTGACAAATGAACCTGAGCAGGTCAGCGATGAGGAACAGCTGAAGTATGTTGTCAATCAAAGC
GTGGCACAGTCCTTTACTGCGAGTCAGCTCAGATTATGGACCCGGGAATATGACATCAGCGACGTCTGGTGGAGCACGTA
CAATGTGCTGATGAGTGAGCCGGACAGGCTGCAATCGAAAAAGCTGTTCTCAAGCCTGTTTTTCCGGAATGAAGTGAAGC
AGCTTGAACGCAGTGTGTCAAGACAGCTCTATGGTGAACGCATTCAGGGCAGTGTATCGAGAATGGAAACCTTTAACGCA
TGCCCGTTTTCCCATTTTGCGTCACACGGGCTGCATCTGAAGGAACGGCAATTCTTCAAGCTTGAAGCACCGGATATCGG
CCAGCTGTTTCATTCCAGCTTAAAGCTGATTTCAGACAGGCTGCGTGAGCAAAAGCTGGATTGGCGAGATTTAACGAAAG
AGCAGTGCGAGCTGTTTTCTTATGATGCGGTAGAGCGGCTGGCACCGAAACTGCAAAAGGAAATTTTGCTCAGCTCAAAC
CGGCATTATTATGTAAAGGAAAAACTGCAAAAAATTGTGACGCGTGTGTCCGGTATTTTAAGCGAGCATGCGAAAGCGAG
CGGATTCGTACCGATCGGGCTTGAACTGGGCTTCGGAGGAAAAGGGCCGCTTCCGCCGCTGACCTTTCAACTGAAAAACG
GCTGTACGATGGAGCTCGTCGGGCGTATTGACCGTGTGGATAAAGCTGAAAGCTCAAAAGGCCTGCTCCTCAGAATTGTC
GATTATAAATCAAGCGATAAAGGGCTTGACTTAGCGGAAGTATATTACGGATTGGCGCTGCAAATGCTGACGTACCTTGA
CTTATCGATTACACATTCAGCTGACTGGCTCGGAATGAGGGCAACGCCTGCCGGAGTGCTGTATTTCCATATTCATGATC
CGATGATTCAATCTAATCTCCCGCTCGGGCTTGACGAGATTGAACAGGAGATTTTTAAGAAATTTAAAATGAAGGGCCTG
CTCCTCGGCGATCAGGAAGTGGTTCGCCTTATGGATACAACCCTTCAAGAGGGACGGTCAAATATCATCAACGCCGGCTT
GAAAAAAGACGGCTCTCTCAGATCAGACTCGGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCC
GTACCTTCCAAGAAGCAGGCGAACAAATTACCGACGGCCGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGACACCG
TGCACATACTGTGCGTTCAAATCAGTGTGCCAATTTGATGAATCACTAGAGGAAAACGAGTATCGCCCATTAAAAGCTGA
AAAAGACAAGACGATACTTGAGTGGATAAAAAAGGAGGCGGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(306-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

98.799

100

0.988