Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   SE695_RS21520 Genome accession   NZ_CP138419
Coordinates   4320446..4321684 (-) Length   412 a.a.
NCBI ID   WP_000815986.1    Uniprot ID   -
Organism   Escherichia coli strain 30EVA2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4315446..4326684
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SE695_RS21500 dam 4316058..4316894 (-) 837 WP_000742143.1 adenine-specific DNA-methyltransferase -
  SE695_RS21505 damX 4317001..4318287 (-) 1287 WP_319790565.1 cell division protein DamX -
  SE695_RS21510 aroB 4318379..4319467 (-) 1089 WP_000439850.1 3-dehydroquinate synthase -
  SE695_RS21515 aroK 4319524..4320045 (-) 522 WP_000818618.1 shikimate kinase AroK -
  SE695_RS21520 comE 4320446..4321684 (-) 1239 WP_000815986.1 DNA uptake porin HofQ Machinery gene
  SE695_RS21525 hofP 4321596..4322000 (-) 405 WP_001264141.1 DNA utilization protein HofP -
  SE695_RS21530 hofO 4321990..4322430 (-) 441 WP_001055760.1 DNA utilization protein HofO -
  SE695_RS21535 hofN 4322414..4322953 (-) 540 WP_058905867.1 DNA utilization protein HofN -
  SE695_RS21540 hofM 4322953..4323732 (-) 780 WP_001295166.1 DNA utilization protein HofM -
  SE695_RS21545 mrcA 4323852..4326404 (+) 2553 WP_001300707.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44743.26 Da        Isoelectric Point: 6.3183

>NTDB_id=826244 SE695_RS21520 WP_000815986.1 4320446..4321684(-) (comE) [Escherichia coli strain 30EVA2]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSIAWQNNNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSNE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=826244 SE695_RS21520 WP_000815986.1 4320446..4321684(-) (comE) [Escherichia coli strain 30EVA2]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGGCAGGAAGGCAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATAACAATATCGCCCGCCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC
AAAACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACACGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGTAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGCTTGCTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGCCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAATGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.327

100

0.393
  comE Haemophilus influenzae 86-028NP

37.097

100

0.391
  pilQ Vibrio campbellii strain DS40M4

38.005

100

0.388
  pilQ Vibrio cholerae strain A1552

37.53

100

0.376
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.53

100

0.376
  comE Glaesserella parasuis strain SC1401

35.714

100

0.364
  pilQ Pseudomonas aeruginosa PAK

34.174

100

0.362