Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QJS82_RS11875 Genome accession   NZ_CP124526
Coordinates   2783714..2785423 (+) Length   569 a.a.
NCBI ID   WP_281490623.1    Uniprot ID   -
Organism   Psychrobacter sp. WB2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2778714..2790423
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QJS82_RS11850 (QJS82_11850) rimP 2780193..2780693 (-) 501 WP_201564284.1 ribosome maturation factor RimP -
  QJS82_RS11865 (QJS82_11865) secG 2781813..2782121 (-) 309 WP_025652589.1 preprotein translocase subunit SecG -
  QJS82_RS11870 (QJS82_11870) tpiA 2782379..2783179 (-) 801 WP_281490622.1 triose-phosphate isomerase -
  QJS82_RS11875 (QJS82_11875) pilB 2783714..2785423 (+) 1710 WP_281490623.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QJS82_RS11880 (QJS82_11880) - 2785653..2786876 (+) 1224 Protein_2324 type II secretion system F family protein -
  QJS82_RS11885 (QJS82_11885) pilD 2786902..2787801 (+) 900 WP_281490624.1 A24 family peptidase Machinery gene
  QJS82_RS11890 (QJS82_11890) coaE 2787919..2788608 (+) 690 WP_201620042.1 dephospho-CoA kinase -
  QJS82_RS11895 (QJS82_11895) rlmB 2788571..2789341 (-) 771 WP_183619390.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  QJS82_RS11900 (QJS82_11900) - 2789616..2789951 (-) 336 WP_068407914.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63146.53 Da        Isoelectric Point: 5.0782

>NTDB_id=826222 QJS82_RS11875 WP_281490623.1 2783714..2785423(+) (pilB) [Psychrobacter sp. WB2]
MSITASKYGGLAQQLISEGIVSEAHMKTAQTESQQQQIGLVPYLVDHKLANAYQLAQMLSQAFGDPLFDLDALSSDVIPK
DLVDEKIVRKFNALPLFKRGQRLFVALSDPTRIDAIDAIAFNSRLSIETVVVEEDKLKKRIENLYADTMQHFDSFSDNDL
NIGFDESEEDEGETKLSDGVDEAPVVKFVNKMLVDAIRMGASDLHFEPYEKTFRVRFRVDGVMQKMASPPVQLASKIAAR
LKVMSQMDISERRIPQDGRIKLKISKDKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEPDQKEMFLEALHKPQ
GMLLITGPTGSGKTVSLYTGINILNTGETNISTAEDPVEINLEGINQVNVNPKVDLTFANALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKNCKKPINIPRQSLLELGFT
DADLDNPDNIIYEPVGCNECREGYKGRVGIYEVMKVSPDISRIIMEDGNAIDIKDAALRNGFRDLRRSGVLKVLQGVTSI
QEMMRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=826222 QJS82_RS11875 WP_281490623.1 2783714..2785423(+) (pilB) [Psychrobacter sp. WB2]
ATGTCTATTACAGCCAGTAAATACGGCGGTTTAGCACAGCAGTTGATTAGTGAAGGTATTGTGAGCGAAGCGCATATGAA
AACGGCGCAAACGGAGTCACAACAACAGCAAATAGGGCTAGTGCCTTATTTGGTCGACCACAAGCTTGCCAATGCTTATC
AGTTGGCACAGATGTTGTCACAAGCGTTTGGCGACCCTCTTTTTGATCTTGATGCGCTCAGCTCAGACGTCATACCAAAA
GACTTGGTCGATGAAAAAATCGTGCGTAAGTTTAATGCCTTACCGCTTTTTAAGCGCGGACAGCGCTTATTCGTGGCATT
GAGTGACCCGACCCGTATTGATGCCATCGACGCCATTGCCTTTAACTCACGCCTATCTATTGAAACCGTCGTCGTTGAAG
AAGATAAGTTAAAAAAGCGTATTGAAAACCTTTACGCCGATACCATGCAGCATTTTGACAGCTTTTCTGATAACGATTTA
AATATTGGATTTGATGAAAGCGAAGAAGACGAAGGTGAAACCAAGCTTAGTGACGGTGTCGATGAAGCACCGGTTGTAAA
GTTCGTCAATAAAATGCTGGTTGACGCCATTCGTATGGGCGCCTCAGACTTACATTTTGAACCCTACGAGAAAACCTTTC
GGGTGCGCTTCCGTGTCGATGGTGTGATGCAAAAAATGGCCAGCCCGCCGGTACAACTGGCGAGTAAGATTGCGGCACGC
TTAAAAGTGATGTCGCAGATGGACATCTCCGAGCGCCGTATACCGCAGGATGGCCGTATTAAGCTTAAGATATCTAAAGA
TAAAGCCATCGATTTCCGAGTAAACTCTTTACCTACCTTATTTGGCGAAAAACTGGTTTTACGTATCTTGGATCCTTCAT
CAGCCATGCTTGGTATTGAAGCATTAGGTTATGAGCCCGATCAAAAAGAGATGTTTTTAGAAGCCTTACATAAGCCGCAA
GGTATGTTACTTATCACCGGTCCTACCGGCTCTGGTAAAACCGTGTCGCTCTACACCGGTATTAATATTTTAAATACCGG
CGAGACCAATATTTCCACTGCCGAAGATCCGGTCGAGATTAACCTTGAAGGTATCAATCAGGTCAACGTCAACCCGAAGG
TGGATTTGACCTTTGCCAATGCGCTTAAATCATTCTTACGGCAAGATCCTGATATCGTTATGGTCGGTGAGATTCGTGAC
CTTGAAACCGCCGAAATTGCGATCAAAGCGGCACAAACGGGACATATGGTGCTGTCGACCCTGCATACAAACTCTGCACC
TGAAACTTTAACGCGCTTACGTAATATGGGTGTTGCCTCATTTAATATCGCAACGTCAGTAAACTTGGTCATTGCTCAGC
GGTTGGCACGGCGCTTATGTAAGAACTGTAAAAAACCAATCAATATCCCGCGTCAAAGCCTGCTTGAACTGGGCTTTACC
GATGCTGATTTAGACAATCCAGACAATATTATTTATGAGCCTGTCGGCTGTAACGAATGCCGTGAAGGTTATAAAGGGCG
TGTTGGTATTTACGAAGTGATGAAAGTCAGCCCTGATATCTCCCGTATTATTATGGAAGATGGTAACGCGATAGACATTA
AAGATGCGGCACTCAGAAACGGCTTTAGAGACTTACGCCGCTCTGGTGTTTTAAAGGTGTTACAAGGGGTGACCAGTATT
CAAGAAATGATGCGCGTGACCTCTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-149)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

68.134

99.824

0.68
  pilB Acinetobacter baylyi ADP1

64.55

99.649

0.643
  pilB Legionella pneumophila strain ERS1305867

51.394

100

0.518
  pilF Neisseria gonorrhoeae MS11

49.735

99.297

0.494
  pilB Vibrio parahaemolyticus RIMD 2210633

48.063

99.824

0.48
  pilB Vibrio cholerae strain A1552

50.282

93.322

0.469
  pilB Vibrio campbellii strain DS40M4

47.834

97.364

0.466