Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   R7234_RS06025 Genome accession   NZ_CP137737
Coordinates   1212273..1213766 (+) Length   497 a.a.
NCBI ID   WP_000249333.1    Uniprot ID   -
Organism   Escherichia coli strain HNSQ2209     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1207273..1218766
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R7234_RS06005 (R7234_06005) pilD 1207923..1208732 (+) 810 WP_001372632.1 prepilin peptidase PppA Machinery gene
  R7234_RS06010 (R7234_06010) gspS2 1208798..1209208 (+) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  R7234_RS06015 (R7234_06015) gspC 1209224..1210183 (+) 960 WP_032084340.1 type II secretion system protein GspC -
  R7234_RS06020 (R7234_06020) gspD 1210213..1212273 (+) 2061 WP_063100683.1 type II secretion system secretin GspD -
  R7234_RS06025 (R7234_06025) pilB/pilB1 1212273..1213766 (+) 1494 WP_000249333.1 type II secretion system ATPase GspE Machinery gene
  R7234_RS06030 (R7234_06030) gspF 1213766..1214989 (+) 1224 WP_001173400.1 type II secretion system inner membrane protein GspF -
  R7234_RS06035 (R7234_06035) gspG 1215006..1215461 (+) 456 WP_001087292.1 type II secretion system major pseudopilin GspG -
  R7234_RS06040 (R7234_06040) gspH 1215465..1216028 (+) 564 WP_001115157.1 type II secretion system minor pseudopilin GspH -
  R7234_RS06045 (R7234_06045) gspI 1216025..1216396 (+) 372 WP_000820099.1 type II secretion system minor pseudopilin GspI -
  R7234_RS06050 (R7234_06050) gspJ 1216393..1216998 (+) 606 WP_032084338.1 type II secretion system minor pseudopilin GspJ -
  R7234_RS06055 (R7234_06055) gspK 1216995..1217972 (+) 978 WP_032084337.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55272.43 Da        Isoelectric Point: 6.0089

>NTDB_id=823938 R7234_RS06025 WP_000249333.1 1212273..1213766(+) (pilB/pilB1) [Escherichia coli strain HNSQ2209]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQPVPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSTGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=823938 R7234_RS06025 WP_000249333.1 1212273..1213766(+) (pilB/pilB1) [Escherichia coli strain HNSQ2209]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTCCACCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAAGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAGCTGATGGAAGATCTCGGTTCTGCCGAGGACTTTTTTACCCTCGCAGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGCGTTGACGGCACATTGCATGAAATGCTGCGA
CCGGGGCGCAAACTGGCCTCGTTGCTGGTATCGCGTATCAAGGTGATGGCACGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGACGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCATCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGTTGAGCCATGAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGTATCTTTCTGGTTACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTTGGGATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTTCAGGCTTCACTTACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACAGCGGTGGGGGCGATCACACGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGCCCCGACTGCCGCCAGCCCGTGCCTGCC
ACCGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCGAACCGTCACTCTGTACCATCCGCAGGGCTGTCCCGCCTG
TAATCACAAAGGCTTTCGCGGACGTACCGCCATCCATGAGCTGATCGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTACGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.765

85.513

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Acinetobacter baumannii D1279779

46.366

80.282

0.372
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

45.25

80.483

0.364