Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   R7234_RS06005 Genome accession   NZ_CP137737
Coordinates   1207923..1208732 (+) Length   269 a.a.
NCBI ID   WP_001372632.1    Uniprot ID   -
Organism   Escherichia coli strain HNSQ2209     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1202923..1213732
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R7234_RS06000 (R7234_06000) sslE 1203158..1207729 (+) 4572 WP_318342176.1 lipoprotein metalloprotease SslE -
  R7234_RS06005 (R7234_06005) pilD 1207923..1208732 (+) 810 WP_001372632.1 prepilin peptidase PppA Machinery gene
  R7234_RS06010 (R7234_06010) gspS2 1208798..1209208 (+) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  R7234_RS06015 (R7234_06015) gspC 1209224..1210183 (+) 960 WP_032084340.1 type II secretion system protein GspC -
  R7234_RS06020 (R7234_06020) gspD 1210213..1212273 (+) 2061 WP_063100683.1 type II secretion system secretin GspD -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 29339.92 Da        Isoelectric Point: 8.3793

>NTDB_id=823937 R7234_RS06005 WP_001372632.1 1207923..1208732(+) (pilD) [Escherichia coli strain HNSQ2209]
MHFDVFQQYPAVMPILATVGGLIIGSFLNVVIWRYPIMLRQQMAEFQGETPSAQSKISLALPRSHCPHCQQTIRVRDNIP
LLSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASIIDLDNQWLPDVFTQGVLWT
GLIAAWAQQSPLTLQDAVTGVLVGFISFYSLRWIAGIVLRKEALGMGDVLLFAALGGWVGALSLPNVALIASCCGLIYAV
ITKKGSTTLPFGPCLSLGGIATLYLQALL

Nucleotide


Download         Length: 810 bp        

>NTDB_id=823937 R7234_RS06005 WP_001372632.1 1207923..1208732(+) (pilD) [Escherichia coli strain HNSQ2209]
ATGCATTTTGATGTTTTTCAGCAATATCCAGCGGTGATGCCCATCCTGGCAACCGTTGGTGGATTAATCATCGGCAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCCATCATGCTTCGCCAACAAATGGCGGAGTTTCAGGGTGAAACGCCGAGTGCGC
AGTCAAAAATAAGCCTGGCGCTGCCACGCTCGCACTGTCCGCATTGCCAGCAGACCATCCGCGTTCGTGACAATATTCCG
CTGCTCTCCTGGCTGATGCTCAAAGGGCGCTGCCGTGACTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTGGAGTT
ATTGACGGCACTCGCTTTTTTGCTGGCGAGTCTGGTCTGGCCGGAAAGTGGATGGGGGCTGGCGGTGATGATATTATCCG
CCTGGCTGATTGCCGCGAGCATCATCGACCTCGATAACCAATGGCTCCCCGATGTCTTTACTCAGGGCGTATTGTGGACG
GGGCTGATTGCGGCATGGGCGCAGCAGAGTCCGCTCACGCTACAAGACGCAGTTACTGGCGTACTGGTAGGGTTTATCTC
TTTTTACTCGCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTACTCTTCGCCG
CTTTAGGTGGCTGGGTGGGGGCGTTGTCGCTGCCCAATGTTGCTTTAATCGCCTCATGCTGCGGCCTGATATATGCCGTT
ATTACAAAGAAAGGATCAACCACGCTGCCTTTTGGACCGTGTTTAAGTCTGGGCGGTATAGCAACACTTTATCTACAGGC
ATTGCTTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(130-239)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

46.442

99.257

0.461
  pilD Vibrio campbellii strain DS40M4

41.026

100

0.416
  pilD Neisseria gonorrhoeae MS11

39.922

95.911

0.383
  pilD Acinetobacter nosocomialis M2

37.736

98.513

0.372
  pilD Acinetobacter baumannii D1279779

37.358

98.513

0.368