Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   R5028_RS28115 Genome accession   NZ_CP137486
Coordinates   5996618..5998321 (-) Length   567 a.a.
NCBI ID   WP_023100230.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain HPA2660     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5991618..6003321
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5028_RS28075 (R5028_28075) - 5991787..5992212 (+) 426 WP_016562341.1 GNAT family N-acetyltransferase -
  R5028_RS28080 (R5028_28080) - 5992209..5992679 (+) 471 WP_023103507.1 hypothetical protein -
  R5028_RS28085 (R5028_28085) - 5992676..5993365 (+) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  R5028_RS28090 (R5028_28090) - 5993471..5993680 (+) 210 WP_003094660.1 hypothetical protein -
  R5028_RS28095 (R5028_28095) yacG 5993717..5993917 (-) 201 WP_017148551.1 DNA gyrase inhibitor YacG -
  R5028_RS28100 (R5028_28100) coaE 5993914..5994525 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  R5028_RS28105 (R5028_28105) pilD 5994522..5995394 (-) 873 WP_017148550.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  R5028_RS28110 (R5028_28110) pilC 5995397..5996614 (-) 1218 WP_023103506.1 type II secretion system F family protein Machinery gene
  R5028_RS28115 (R5028_28115) pilB 5996618..5998321 (-) 1704 WP_023100230.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R5028_RS28120 (R5028_28120) pilE 5998548..5999084 (+) 537 WP_033945690.1 pilin Machinery gene
  R5028_RS28125 (R5028_28125) tfpZ 5999149..5999886 (+) 738 WP_023110409.1 TfpX/TfpZ family type IV pilin accessory protein -
  R5028_RS28135 (R5028_28135) nadC 6000120..6000968 (-) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62580.51 Da        Isoelectric Point: 5.5586

>NTDB_id=820615 R5028_RS28115 WP_023100230.1 5996618..5998321(-) (pilB) [Pseudomonas aeruginosa strain HPA2660]
MNDTIQLSGLARQLVLHELLDEKAAQQAQLQAQRNKLSLVTYLVQSKLVKGQALTELAADQFGIAYCDLNSLDRDSLPKD
LISEKLVRQHRVVPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGIIIEKLFENATDSLAGLDDVDLE
GLDVGSGAGASQDDDSSAETDDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPKETLLHEGFP
EDRIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRASGLLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=820615 R5028_RS28115 WP_023100230.1 5996618..5998321(-) (pilB) [Pseudomonas aeruginosa strain HPA2660]
ATGAACGATACCATCCAGCTCAGCGGCCTGGCTCGCCAGCTCGTCCTGCATGAACTTCTCGATGAAAAAGCAGCTCAACA
GGCCCAGTTGCAAGCCCAGCGCAACAAGTTGTCGCTGGTAACCTATCTGGTACAGAGCAAGCTAGTGAAAGGACAGGCCT
TGACCGAACTGGCGGCGGATCAATTCGGTATCGCCTACTGCGACCTGAACAGCCTTGATCGGGACAGCCTGCCCAAGGAT
CTGATCAGCGAGAAGCTGGTGCGCCAGCATCGGGTAGTTCCGTTGTGGCGACGCGGCAACAAGCTGTTCGTCGGCATCTC
CGATCCAGCCAACCACCAGGCGATTAACGATGTGCAGTTCAGCACAGGCCTGACTACTGAAGCCATCCTGGTCGAGGATG
ACAAGCTCGGCATCATCATCGAGAAACTATTCGAAAATGCCACTGACAGCCTTGCAGGCCTGGATGACGTGGATCTTGAA
GGCCTGGATGTCGGTTCCGGTGCGGGCGCAAGCCAGGATGATGATTCCAGTGCAGAGACAGACGACGCCCCGGTGGTGCG
TTTCGTCAACAAAATGTTATTGGATGCGATCAGAGGCGGCTCCTCTGATCTGCATTTCGAGCCCTATGAAAAGATTTACC
GTGTACGTTTCCGTACCGATGGCATGCTCCATGAGGTAGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATTTCCGCGCGA
CTCAAGGTAATGGCAGGCCTGGACATTTCCGAACGGCGTAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGAC
CAAGTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGTATCCTCGATTCCTCCA
GCGCGCAGATGGGCATCGACGCCCTCGGCTACGAGGAAGACCAGAAGGAACTCTACCTGGCTGCGCTTAAGCAGCCGCAG
GGCATGATCCTGGTCACCGGGCCCACCGGCTCGGGCAAGACGGTCTCCCTGTACACCGGCCTGAACATCCTCAACACCAC
CGACATCAACATCTCCACTGCCGAAGACCCGGTGGAAATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCC
AAGGTATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATTCGCGAC
CTGGAGACGGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
CGAGACCCTGACCCGGTTGCTGAACATGGGCGTGCCGGCGTTCAACCTAGCGACCTCGGTGAACCTGATCATCGCCCAGC
GACTTGCGCGAAAACTCTGCTCGCACTGCAAGAAAGAGCATGAGGTACCGAAGGAAACACTCCTCCATGAAGGCTTCCCC
GAGGACCGGATCGGCACCTTCAAGCTGTATTCACCAGTAGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAAC
AGGCCCGCAAAGAAGGCTTCAACGATCTACGCGCTTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAAGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.07

100

0.584
  pilB Acinetobacter baylyi ADP1

57.447

99.471

0.571
  pilB Legionella pneumophila strain ERS1305867

55.282

100

0.554
  pilF Neisseria gonorrhoeae MS11

50.444

99.295

0.501
  pilB Vibrio parahaemolyticus RIMD 2210633

51.763

95.062

0.492
  pilB Vibrio cholerae strain A1552

50.545

97.002

0.49
  pilB Vibrio campbellii strain DS40M4

50.832

95.414

0.485
  pilF Thermus thermophilus HB27

38.584

99.647

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.189

93.474

0.376