Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   P7V53_RS28470 Genome accession   NZ_CP123228
Coordinates   6008002..6009720 (+) Length   572 a.a.
NCBI ID   WP_280152853.1    Uniprot ID   -
Organism   Piscinibacter sp. XHJ-5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6003002..6014720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P7V53_RS28435 proB 6003281..6004399 (-) 1119 WP_280152849.1 glutamate 5-kinase -
  P7V53_RS28440 cgtA 6004396..6005472 (-) 1077 WP_280156637.1 Obg family GTPase CgtA -
  P7V53_RS28445 - 6005469..6005657 (-) 189 WP_280152850.1 DUF5668 domain-containing protein -
  P7V53_RS28450 rpmA 6005771..6006028 (-) 258 WP_280152851.1 50S ribosomal protein L27 -
  P7V53_RS28455 rplU 6006054..6006365 (-) 312 WP_280156638.1 50S ribosomal protein L21 -
  P7V53_RS28460 ispB 6006589..6007530 (+) 942 WP_280152852.1 octaprenyl diphosphate synthase -
  P7V53_RS28470 pilB 6008002..6009720 (+) 1719 WP_280152853.1 type IV-A pilus assembly ATPase PilB Machinery gene
  P7V53_RS28475 pilC 6009782..6011005 (+) 1224 WP_280152854.1 type II secretion system F family protein Machinery gene
  P7V53_RS28480 pilD 6011002..6011853 (+) 852 WP_280152855.1 A24 family peptidase Machinery gene
  P7V53_RS28485 coaE 6011870..6012502 (+) 633 WP_280152856.1 dephospho-CoA kinase -
  P7V53_RS28490 zapD 6012579..6013334 (+) 756 WP_280152857.1 cell division protein ZapD -
  P7V53_RS28495 - 6013346..6013549 (+) 204 WP_280152858.1 DNA gyrase inhibitor YacG -
  P7V53_RS28500 - 6013562..6014482 (-) 921 WP_280152859.1 caspase family protein -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62225.50 Da        Isoelectric Point: 6.0047

>NTDB_id=820104 P7V53_RS28470 WP_280152853.1 6008002..6009720(+) (pilB) [Piscinibacter sp. XHJ-5]
MEALSDAPQSTLSGVARVLVHAGRLSLKTAEDLVKGAKEKKINFVASVLSAGAITPSELAHTLSGSLALPLLDLNAVDSQ
RLPRNIIDGKLSAQYQVVVLGKRGNRLFIGGSDPTDQEAVERIKFATQLTPEWVIVEHDKLLKILDATGGSASEALESIA
SGDFEFDVTDDVTSQEAADTSSSEVEDAPVVRFLQKMLIDAINARASDLHFEPYEYHYRVRFRIDGELREITQPPIAIKD
KLASRIKVISRLDISEKRVPQDGRMKLKFGSKAIDFRVSTLPTLFGEKIVIRILDASSAKLGIEALGYEKIEKDRLMGAI
QRPYGMILVTGPTGSGKTVSLYTCLNILNQPGVNISTVEDPAEINLPGINQVNVNDKAGMNFAVALKSFLRQDPDVIMVG
EIRDLETADIAIKAAQTGHLVMSTLHTNDAPTTLTRLMNMGVAPFNIAASVLLITAQRLARKLCENCKQPAEYPREAMLK
AGFSEKDLDGTWKPYRAVGCSACSNGYKGRVGIYQVMPISEEIQRIILAEGTSMDIAKQAQVEGVRDLRQSGLVKVRAGV
TTLEEVISVTNE

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=820104 P7V53_RS28470 WP_280152853.1 6008002..6009720(+) (pilB) [Piscinibacter sp. XHJ-5]
ATGGAAGCACTCAGCGACGCACCGCAGTCCACGCTCTCCGGGGTCGCTCGCGTTCTCGTCCATGCCGGACGGCTGAGCCT
CAAGACCGCGGAAGACCTGGTCAAGGGCGCCAAAGAGAAGAAGATCAACTTTGTCGCATCGGTGCTGTCGGCCGGCGCGA
TTACCCCGTCCGAGCTCGCGCACACCTTGTCCGGCTCGCTCGCGCTCCCCCTGCTCGACCTCAATGCCGTCGACTCGCAA
AGGCTGCCGCGCAACATCATCGACGGCAAGCTCTCGGCGCAGTACCAGGTGGTGGTGCTGGGCAAGCGGGGCAACCGACT
GTTCATCGGCGGCTCCGACCCGACCGATCAAGAGGCTGTCGAGCGCATCAAGTTCGCCACCCAGCTGACGCCGGAATGGG
TGATCGTCGAGCACGACAAGCTGCTCAAGATCCTCGACGCCACGGGCGGCAGCGCAAGCGAAGCGCTCGAGTCGATCGCA
TCCGGCGATTTCGAATTCGACGTCACCGACGACGTCACCTCCCAGGAGGCGGCGGATACGTCATCGTCGGAGGTCGAGGA
CGCCCCGGTCGTACGCTTCCTGCAGAAGATGCTGATCGACGCCATCAACGCGCGCGCATCCGACCTGCATTTCGAGCCCT
ACGAGTACCACTACCGCGTGCGCTTCCGTATCGACGGCGAGCTGCGCGAGATCACACAACCGCCGATCGCGATCAAGGAC
AAGCTCGCGTCGCGCATCAAGGTCATCTCCCGGCTGGACATCTCCGAAAAACGCGTGCCGCAGGACGGCCGCATGAAGCT
CAAGTTCGGCAGCAAGGCCATCGACTTCCGGGTCAGCACCCTGCCGACGCTGTTCGGCGAGAAGATCGTGATCCGGATCC
TCGACGCGTCGAGCGCCAAGCTTGGTATCGAGGCGCTCGGCTACGAGAAGATCGAGAAGGACCGCCTGATGGGCGCGATC
CAGCGTCCTTACGGCATGATCCTCGTGACCGGCCCCACCGGTTCGGGCAAGACGGTGTCGCTGTACACCTGCCTCAACAT
CCTCAATCAGCCGGGCGTCAACATCTCGACGGTCGAAGACCCGGCGGAAATCAACTTGCCGGGCATCAACCAGGTCAACG
TCAACGACAAGGCCGGCATGAACTTCGCCGTGGCGCTGAAGTCGTTCCTGCGGCAGGACCCGGACGTCATCATGGTCGGC
GAAATCCGCGACCTCGAAACCGCCGACATCGCGATCAAGGCCGCGCAAACGGGTCACCTGGTCATGTCGACGCTGCACAC
CAACGACGCGCCGACGACGCTGACCCGTCTGATGAACATGGGGGTGGCGCCATTCAATATCGCTGCCAGCGTGCTGCTCA
TCACCGCGCAGCGGCTTGCGCGCAAGCTGTGCGAGAACTGCAAGCAGCCGGCGGAGTACCCGCGCGAGGCGATGTTGAAG
GCCGGCTTCTCCGAGAAGGACCTTGACGGGACGTGGAAGCCGTACCGCGCGGTCGGCTGCTCGGCGTGCAGCAACGGCTA
CAAGGGCCGGGTCGGCATCTACCAGGTGATGCCCATCAGCGAGGAGATCCAACGCATCATCCTGGCCGAGGGCACCTCGA
TGGACATCGCCAAGCAGGCACAGGTGGAGGGCGTGCGCGACCTGCGTCAGTCGGGTCTCGTGAAGGTGCGTGCCGGTGTC
ACCACACTCGAGGAAGTGATCTCGGTCACCAACGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-151)

Type II/IV secretion system protein (T2SSE)

(196-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.833

100

0.54
  pilB Acinetobacter baylyi ADP1

52.613

100

0.528
  pilF Neisseria gonorrhoeae MS11

53.464

98.427

0.526
  pilB Legionella pneumophila strain ERS1305867

50.441

99.126

0.5
  pilB Vibrio cholerae strain A1552

46.975

98.252

0.462
  pilB Vibrio parahaemolyticus RIMD 2210633

45.907

98.252

0.451
  pilB Vibrio campbellii strain DS40M4

45.357

97.902

0.444
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.623

92.657

0.367