Detailed information NTG0820009

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QEP69_RS07775
• Genome accession: NZ_CP123209
• Coordinates: 1762734..1763903 (-)
• Length: 389 a.a.
• NCBI ID: WP_005732021.1
• Uniprot ID: A0A108V2M6
• Organism: Pseudomonas amygdali pv. aesculi strain 1804
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1757734..1768903

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QEP69_RS07760	yejB	1759018..1760082 (+)	1065	WP_010215968.1	microcin C ABC transporter permease YejB	-
QEP69_RS07765	-	1760082..1761104 (+)	1023	WP_005732025.1	ABC transporter permease	-
QEP69_RS07770	-	1761106..1762683 (+)	1578	WP_010206223.1	ABC transporter ATP-binding protein	-
QEP69_RS07775	pilU	1762734..1763903 (-)	1170	WP_005732021.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
QEP69_RS07780	-	1764027..1764302 (+)	276	WP_005732020.1	peptidylprolyl isomerase	-
QEP69_RS07785	pgm	1764377..1766023 (-)	1647	WP_005732019.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
QEP69_RS07790	-	1766139..1768466 (-)	2328	WP_010206225.1	ATP-dependent DNA helicase	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42650.81 Da
• Isoelectric Point: 5.8676

>NTDB_id=820009 QEP69_RS07775 WP_005732021.1 1762734..1763903(-) (pilU) [Pseudomonas amygdali pv. aesculi strain 1804]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETLKPGEVAVIAQGLMDEEQKLEFLRELEMNLAVSLPGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGVASLTTPPPAPTGSSTASTAEWGLVDDDEPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=820009 QEP69_RS07775 WP_005732021.1 1762734..1763903(-) (pilU) [Pseudomonas amygdali pv. aesculi strain 1804]
ATGGATTTTCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTGTATCTTTCCACCGGAGCGCCTCCGTG
CGCCAAGTTCAACGGCGTGCTCAAGCCGTTGGGCAGTGAAACCCTCAAACCGGGCGAGGTGGCGGTGATTGCCCAGGGGC
TGATGGACGAGGAGCAGAAGCTCGAATTCCTGCGCGAACTGGAGATGAACCTTGCTGTTTCGCTGCCGGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGTAACGAGGTGTCCATCGTCGCGCGGAATATCAAGCTGGATATTCCGCGTTTCGA
GGACCTGTTCCTGCCGCCCGTACTGCTCGATGTGATCATGGAAAAGCACGGGCTGGTGTTGTTCGTCGGTGCCACCGGTT
CGGGCAAATCGACCTCGCTGGCGGCGCTGATCGATTACCGCAATCGCAACGCCAGCGGCCATATCATCACCATTGAAGAC
CCGGTCGAATTCATCCATCGGCACAAGAAGTCCATCGTCAACCAGCGCGAGGTGGGGGTCGATACCCGCAGTTTCCGCGC
GGCGCTGAAGAACACGTTGCGTCAGGCTCCGGATGTGATTCTGATCGGCGAAATTCGTGACCGCGAGACCATGGAGCATG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCGATCTCGACCCTGCATGCCAACAACGCCAATCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGAAGAGCGTCGTGCGCAGTTGCTGCATGACCTGGGCAATAACCTCAAGGCGTTCGTGTCCCAGCG
GCTGGTTAGAACCCCGGACGGCAAACGTCGCGCAGCGGTGGAAGTGATGATGGGCACGCCGACCATTCGCGACCTGATTC
AGCGCAATGAACTGACCGAGCTCAAGGGCATCATGGAGAAATCCGGCAGCCTCGGTATGCAGACCTTCGACACGGCGTTG
TTCAATCTGGCCGTCGAGGGGGCGATCAGCGAGGAGGAGGCGCTGAAGAATGCCGACTCGCAGAACAACGTTCGTCTGCG
CCTCAAACTGCACAGTGAAGGCGGAGTCGCCAGCCTGACGACGCCGCCCCCGGCCCCGACCGGCAGCAGCACGGCAAGTA
CCGCCGAGTGGGGGCTGGTCGATGACGACGAGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-270)

3D structure

• AlphaFold DB: A0A108V2M6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.072	99.486	0.558
pilU	Acinetobacter baylyi ADP1	51.541	91.774	0.473
pilU	Vibrio cholerae strain A1552	51.714	89.974	0.465
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.687	98.458	0.391
pilT	Pseudomonas aeruginosa PAK	41.399	88.175	0.365