Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QEN58_RS08635 Genome accession   NZ_CP122961
Coordinates   1913619..1915376 (-) Length   585 a.a.
NCBI ID   WP_280106691.1    Uniprot ID   -
Organism   Halomonas alkaliantarctica strain MSP3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1908619..1920376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QEN58_RS08605 (QEN58_08605) - 1909268..1909609 (+) 342 WP_280106685.1 hypothetical protein -
  QEN58_RS08610 (QEN58_08610) - 1909638..1910525 (-) 888 WP_280106686.1 DNA replication terminus site-binding protein -
  QEN58_RS08615 (QEN58_08615) yacG 1910565..1910798 (-) 234 WP_280106687.1 DNA gyrase inhibitor YacG -
  QEN58_RS08620 (QEN58_08620) coaE 1910833..1911435 (-) 603 WP_280106688.1 dephospho-CoA kinase -
  QEN58_RS08625 (QEN58_08625) - 1911489..1912334 (-) 846 WP_280106689.1 A24 family peptidase -
  QEN58_RS08630 (QEN58_08630) pilC 1912383..1913615 (-) 1233 WP_280106690.1 type II secretion system F family protein Machinery gene
  QEN58_RS08635 (QEN58_08635) pilB 1913619..1915376 (-) 1758 WP_280106691.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QEN58_RS08640 (QEN58_08640) - 1915647..1916096 (+) 450 WP_280106918.1 pilin -
  QEN58_RS08645 (QEN58_08645) - 1916170..1916625 (+) 456 WP_280106692.1 pilin -
  QEN58_RS08650 (QEN58_08650) - 1916702..1917136 (+) 435 WP_280106693.1 anti-virulence regulator CigR family protein -
  QEN58_RS08655 (QEN58_08655) - 1917228..1918049 (+) 822 WP_280106694.1 inositol monophosphatase family protein -
  QEN58_RS08665 (QEN58_08665) - 1918366..1919523 (-) 1158 WP_280106695.1 alpha-hydroxy acid oxidase -

Sequence

Protein


Download         Length: 585 a.a.        Molecular weight: 64121.56 Da        Isoelectric Point: 6.0978

>NTDB_id=817553 QEN58_RS08635 WP_280106691.1 1913619..1915376(-) (pilB) [Halomonas alkaliantarctica strain MSP3]
MSQPPFESTLSHAAARGGLRGIAQRLVKHGLLNDAQAATAETTASDLEISLLQHVIESGLVDPNEATLAAGWEYGLPVID
LEAVRLTALPPAADYPVKVLQSLNVLPLARHGHRLTVAVPYPATLTQLDELQFATGLSVEGILAPVDQITVALNSYLAQN
ERGMLDELDDIDDAVSSLSIVQNNDGNEVPLEESSQNSEDAPIVKFVNKILLDAIRRGASDIHFEPYETLYRVRFRVDGM
LIEVARPPFAMRNRIAARLKIMARLDISERRLPQDGSIKLKLSRTRSLDFRVNSLPTVYGEKLVLRILDPTAVQLGIEQL
GFTDDQRGHYEHALKQPQGMILVTGPTGSGKTVSLYTGINILNKVERNICTAEDPVEIKVAGINQVNVLPKIGLDFASAL
RAFLRQDPDVVMVGEIRDLETAEIAVKAAQTGHLVLSTVHTNSAAETLTRLANMGVSSFNIASAVSLIIAQRLARKLCTH
CKEPIDIPHEALRKEGFSSEEIHQATIYKPVGCKHCTHGYKGRIGIYEVVPITEAMRQLIMRDANSLDIDQQVCKEGYPT
LHRSGLLKVMQGITSLEEINRVSKE

Nucleotide


Download         Length: 1758 bp        

>NTDB_id=817553 QEN58_RS08635 WP_280106691.1 1913619..1915376(-) (pilB) [Halomonas alkaliantarctica strain MSP3]
ATGAGCCAGCCCCCCTTTGAGTCGACTTTAAGTCACGCCGCCGCCCGCGGCGGCCTGCGCGGCATTGCCCAGCGTTTGGT
TAAGCACGGCTTGCTGAACGATGCCCAGGCCGCCACTGCGGAAACGACCGCATCAGACTTGGAAATATCGTTACTTCAGC
ATGTTATCGAGAGCGGCCTGGTTGACCCTAATGAAGCCACACTGGCGGCCGGGTGGGAGTATGGGCTACCGGTCATAGAT
TTGGAGGCGGTGCGGCTTACCGCGCTACCTCCTGCGGCTGACTATCCTGTTAAAGTTTTGCAGAGCCTTAACGTTTTGCC
ATTAGCGCGCCACGGCCATCGCCTGACGGTTGCGGTGCCTTATCCCGCCACTTTGACGCAACTGGATGAATTGCAGTTTG
CGACCGGCCTTAGTGTTGAAGGTATCCTCGCGCCCGTTGATCAAATAACGGTCGCCCTCAATAGCTACCTTGCGCAAAAC
GAGCGCGGCATGCTGGATGAGCTGGACGATATTGATGATGCGGTCAGCTCGCTGAGCATTGTGCAGAATAACGACGGCAA
TGAAGTACCGCTGGAAGAGTCTTCCCAAAATAGCGAAGACGCTCCCATCGTTAAATTTGTGAACAAGATTCTGCTAGATG
CGATCCGTCGTGGCGCGTCCGATATCCATTTTGAGCCTTACGAAACGCTGTATCGGGTACGCTTTCGGGTTGATGGAATG
CTAATCGAAGTGGCACGACCACCCTTTGCTATGCGTAACCGTATCGCGGCCCGACTGAAAATCATGGCGCGCCTTGATAT
CTCGGAACGTCGCTTACCCCAAGATGGCTCGATCAAGCTCAAGCTTTCCCGCACACGCTCCCTCGACTTCCGGGTTAACT
CACTGCCTACCGTCTATGGCGAAAAGTTGGTGTTGCGGATATTAGACCCTACCGCAGTGCAGCTAGGTATTGAGCAACTG
GGCTTTACCGACGACCAACGGGGCCATTATGAGCACGCATTAAAACAACCCCAAGGCATGATTCTGGTCACCGGCCCTAC
CGGTAGTGGTAAAACCGTTTCGCTGTATACCGGCATTAATATTCTTAATAAAGTGGAACGTAATATCTGTACAGCCGAAG
ACCCGGTTGAAATCAAAGTCGCGGGTATCAATCAGGTGAACGTATTACCTAAGATCGGCCTCGATTTTGCCAGTGCGCTG
AGAGCGTTTCTACGCCAAGACCCAGACGTCGTTATGGTAGGGGAGATTCGCGATCTGGAAACCGCCGAAATTGCCGTTAA
AGCCGCCCAGACAGGTCACTTGGTACTCTCCACGGTGCATACCAACTCGGCGGCTGAAACACTGACTCGTCTGGCTAATA
TGGGTGTCTCTTCGTTTAACATCGCCAGCGCCGTTAGTTTGATTATTGCTCAGCGCCTGGCTCGCAAACTATGCACACAC
TGCAAAGAGCCCATTGATATTCCCCATGAGGCGCTGCGCAAAGAAGGCTTTAGCAGCGAAGAAATCCATCAGGCAACTAT
CTATAAACCGGTGGGTTGCAAGCACTGCACTCACGGCTATAAAGGCCGAATCGGCATTTATGAGGTAGTGCCGATTACCG
AAGCGATGCGTCAACTGATTATGCGTGACGCCAACTCCTTAGATATTGATCAACAGGTGTGCAAGGAGGGCTATCCAACC
CTTCACCGCAGTGGATTACTGAAAGTCATGCAGGGCATTACCAGCCTGGAAGAGATTAACCGTGTGAGCAAGGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(209-476)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(56-161)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

51.323

96.923

0.497
  pilB Acinetobacter baylyi ADP1

50.526

97.436

0.492
  pilB Acinetobacter baumannii D1279779

50.528

97.094

0.491
  pilB Vibrio campbellii strain DS40M4

51.091

94.017

0.48
  pilB Vibrio cholerae strain A1552

49.909

94.188

0.47
  pilB Vibrio parahaemolyticus RIMD 2210633

49.548

94.53

0.468
  pilF Neisseria gonorrhoeae MS11

49.48

82.222

0.407