Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QEN58_RS01700 Genome accession   NZ_CP122961
Coordinates   351565..352608 (+) Length   347 a.a.
NCBI ID   WP_280105476.1    Uniprot ID   -
Organism   Halomonas alkaliantarctica strain MSP3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 346565..357608
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QEN58_RS01670 (QEN58_01670) - 346908..347357 (-) 450 WP_280105470.1 DUF4426 domain-containing protein -
  QEN58_RS01675 (QEN58_01675) metW 347397..347990 (-) 594 WP_280105471.1 methionine biosynthesis protein MetW -
  QEN58_RS01680 (QEN58_01680) - 347990..349216 (-) 1227 WP_280105472.1 homoserine O-acetyltransferase -
  QEN58_RS01685 (QEN58_01685) - 349286..349879 (-) 594 WP_280105473.1 YggT family protein -
  QEN58_RS01690 (QEN58_01690) proC 349936..350760 (-) 825 WP_280105474.1 pyrroline-5-carboxylate reductase -
  QEN58_RS01695 (QEN58_01695) - 350816..351532 (-) 717 WP_280105475.1 YggS family pyridoxal phosphate-dependent enzyme -
  QEN58_RS01700 (QEN58_01700) pilT 351565..352608 (+) 1044 WP_280105476.1 type IV pilus twitching motility protein PilT Machinery gene
  QEN58_RS01705 (QEN58_01705) pilU 352601..353782 (+) 1182 WP_280105477.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QEN58_RS01710 (QEN58_01710) - 353807..354946 (-) 1140 WP_280105478.1 anhydro-N-acetylmuramic acid kinase -
  QEN58_RS01715 (QEN58_01715) tyrS 355092..356291 (+) 1200 WP_280105479.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38168.63 Da        Isoelectric Point: 5.8177

>NTDB_id=817544 QEN58_RS01700 WP_280105476.1 351565..352608(+) (pilT) [Halomonas alkaliantarctica strain MSP3]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAIDNNDVRKLIYDIMNDRQRRDYEEHLEIDFSFEVPGIAR
FRVNAFTQARGAGAVFRTIPNQVLSMQALGLGEVFERLAMLPRGLVLVTGPTGSGKSTTLAAMIDYINDHRYEHILTIED
PVEFVHASKRCLINQREVHRDTHSFADALRSALREDPDVILVGELRDLETIRLALTAAETGHLVLGTLHTTSAAKTIDRI
IDVFPGEEKAMVRSMLSESLQAVVSQSLLKRQGGGRVAAHEILIATSAVRNLIREDKVAQIYSAIQTGGSLGMQTLDAEL
SRLVKDGTVSLEEAQLSAKGTLAIKDE

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=817544 QEN58_RS01700 WP_280105476.1 351565..352608(+) (pilT) [Halomonas alkaliantarctica strain MSP3]
ATGGATATTACCGAACTGCTAGCATTCTCGGCAAAGCAGAATGCTTCTGACTTGCACCTTTCGGCCGGTTTGCCGCCCAT
GATACGTGTTGATGGCGACATTCGTCGGCTCAATGTGCCCGCTATCGATAATAACGATGTGCGCAAATTGATCTACGACA
TCATGAATGATCGCCAGCGGCGCGACTACGAAGAGCACCTGGAAATTGATTTTTCCTTTGAAGTGCCTGGGATTGCCCGC
TTCCGCGTGAACGCCTTTACGCAAGCGCGCGGGGCGGGGGCTGTGTTTCGCACCATCCCTAATCAAGTGCTCTCCATGCA
AGCCCTGGGGCTCGGTGAGGTGTTCGAGCGCTTGGCAATGTTGCCGCGAGGCCTAGTGCTGGTGACTGGGCCAACGGGGT
CGGGTAAAAGCACCACGCTGGCCGCGATGATCGATTACATTAACGATCATCGTTACGAGCATATTCTTACCATTGAAGAC
CCCGTCGAGTTTGTCCACGCCAGCAAGCGCTGTTTGATAAACCAACGCGAAGTGCACCGCGACACCCATAGCTTTGCCGA
TGCGTTGCGTAGCGCGCTACGTGAAGACCCGGATGTGATCCTGGTCGGCGAGCTGCGTGATCTGGAGACCATCCGTTTAG
CGCTGACCGCGGCTGAAACCGGCCATCTGGTGCTGGGAACGCTGCATACCACCTCTGCCGCCAAGACAATCGACCGGATC
ATCGATGTGTTTCCCGGCGAAGAAAAAGCCATGGTGCGTTCCATGTTATCGGAATCGCTCCAGGCGGTGGTGTCGCAGTC
GCTGCTTAAACGTCAGGGCGGTGGTCGCGTAGCGGCCCACGAAATCCTCATTGCCACTTCTGCCGTGCGCAACTTGATCC
GCGAAGACAAAGTGGCGCAGATCTACTCGGCCATTCAAACCGGCGGCAGCCTGGGCATGCAAACCCTGGATGCCGAGCTG
TCGCGGTTAGTGAAAGACGGTACCGTAAGTTTGGAAGAGGCGCAGCTGAGTGCGAAGGGAACGCTGGCGATAAAAGATGA
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.876

97.695

0.761
  pilT Pseudomonas stutzeri DSM 10701

77.286

97.695

0.755
  pilT Acinetobacter nosocomialis M2

75.516

97.695

0.738
  pilT Acinetobacter baumannii D1279779

75.516

97.695

0.738
  pilT Acinetobacter baumannii strain A118

75.516

97.695

0.738
  pilT Acinetobacter baylyi ADP1

74.926

97.695

0.732
  pilT Legionella pneumophila strain Lp02

71.893

97.406

0.7
  pilT Legionella pneumophila strain ERS1305867

71.893

97.406

0.7
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.851

96.542

0.674
  pilT Vibrio cholerae strain A1552

69.851

96.542

0.674
  pilT Neisseria meningitidis 8013

65.497

98.559

0.646
  pilT Neisseria gonorrhoeae MS11

65.205

98.559

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

56.135

93.948

0.527
  pilU Pseudomonas stutzeri DSM 10701

42.478

97.695

0.415
  pilU Vibrio cholerae strain A1552

41.298

97.695

0.403
  pilU Acinetobacter baylyi ADP1

40.774

96.83

0.395