Detailed information NTG0817545

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QEN58_RS01705
• Genome accession: NZ_CP122961
• Coordinates: 352601..353782 (+)
• Length: 393 a.a.
• NCBI ID: WP_280105477.1
• Uniprot ID: -
• Organism: Halomonas alkaliantarctica strain MSP3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 347601..358782

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QEN58_RS01680 (QEN58_01680)	-	347990..349216 (-)	1227	WP_280105472.1	homoserine O-acetyltransferase	-
QEN58_RS01685 (QEN58_01685)	-	349286..349879 (-)	594	WP_280105473.1	YggT family protein	-
QEN58_RS01690 (QEN58_01690)	proC	349936..350760 (-)	825	WP_280105474.1	pyrroline-5-carboxylate reductase	-
QEN58_RS01695 (QEN58_01695)	-	350816..351532 (-)	717	WP_280105475.1	YggS family pyridoxal phosphate-dependent enzyme	-
QEN58_RS01700 (QEN58_01700)	pilT	351565..352608 (+)	1044	WP_280105476.1	type IV pilus twitching motility protein PilT	Machinery gene
QEN58_RS01705 (QEN58_01705)	pilU	352601..353782 (+)	1182	WP_280105477.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
QEN58_RS01710 (QEN58_01710)	-	353807..354946 (-)	1140	WP_280105478.1	anhydro-N-acetylmuramic acid kinase	-
QEN58_RS01715 (QEN58_01715)	tyrS	355092..356291 (+)	1200	WP_280105479.1	tyrosine--tRNA ligase	-

Sequence

Protein

• Length: 393 a.a.
• Molecular weight: 43409.55 Da
• Isoelectric Point: 6.5973

>NTDB_id=817545 QEN58_RS01705 WP_280105477.1 352601..353782(+) (pilU) [Halomonas alkaliantarctica strain MSP3]
MNKDSSTQTPSVELSPTQWLHQLLDIMVEQHASDLLISVGAPPSLKMPDGLVPLGQQRLTAHQVNELVTHTLPEGLRERF
ASEREANFALSREGKGRFRVSAFQQRNQMAMVVRSIAIEIPRLEALGVPGQLTELAQAKRGLVLVVGGTGTGKSTTLASM
IQQRNETVGGHIISIEDPIEYLHPHKRAIVNQREVGIDTESFEVALKNTLRQAPDVILIGEIRTRETMEHALTFAETGHL
CLATLHANNANQALERIIHFFPHERHEQIWMDLSLNLRAVVAQQLLPTLAGGRRAAIEIMLQSPRIADLIRKGDVAEIKA
AMAKSRDAGMQTFDQALHDLHQAGHISREVAMAHADSANDLRMLLNVSDAKGRNLTLDEQVPSHLGLRDGDDY

Nucleotide

• Length: 1182 bp

>NTDB_id=817545 QEN58_RS01705 WP_280105477.1 352601..353782(+) (pilU) [Halomonas alkaliantarctica strain MSP3]
ATGAATAAAGACAGCTCAACTCAAACGCCATCTGTCGAGCTTTCGCCCACCCAGTGGCTGCACCAACTGCTCGATATCAT
GGTCGAGCAGCACGCTTCCGATTTACTCATTTCCGTGGGTGCGCCGCCAAGCCTAAAAATGCCCGATGGGCTGGTGCCGT
TGGGACAGCAGCGTTTAACCGCCCACCAGGTCAACGAGCTGGTCACCCACACGCTGCCGGAAGGGCTGCGGGAACGTTTT
GCCAGTGAGCGAGAGGCGAATTTTGCCCTCAGCCGCGAGGGCAAAGGGCGTTTTCGGGTCAGTGCTTTCCAGCAGCGCAA
CCAGATGGCGATGGTGGTGCGCAGTATTGCGATTGAGATTCCTCGCCTGGAGGCGCTGGGCGTACCGGGGCAGTTGACTG
AATTAGCCCAAGCCAAGCGTGGGCTGGTGCTGGTGGTAGGCGGCACGGGAACCGGGAAGTCGACAACCCTGGCGTCGATG
ATTCAGCAGCGCAACGAGACCGTCGGCGGCCATATCATCAGTATCGAAGACCCTATCGAGTACCTGCACCCGCATAAGCG
CGCGATTGTGAATCAACGCGAAGTGGGGATTGATACCGAGTCGTTTGAGGTGGCGCTAAAAAATACCCTGCGCCAGGCGC
CGGATGTGATTCTGATTGGTGAAATCCGCACTCGGGAAACCATGGAACATGCGCTGACGTTTGCTGAAACCGGCCATCTC
TGTCTGGCGACACTGCATGCCAATAACGCTAACCAGGCGCTGGAGCGCATCATTCACTTCTTCCCCCATGAACGCCATGA
GCAGATCTGGATGGATCTTTCGTTGAATCTGCGTGCAGTCGTCGCCCAGCAACTGCTACCTACGCTTGCGGGCGGGCGCC
GTGCGGCCATTGAGATCATGCTTCAGTCGCCGCGCATTGCCGACTTGATCCGTAAAGGAGACGTCGCCGAGATTAAAGCA
GCAATGGCCAAGTCCCGTGATGCCGGAATGCAGACCTTCGATCAGGCGTTGCACGACCTGCATCAGGCGGGGCATATCAG
TAGAGAAGTGGCCATGGCCCACGCCGACTCGGCTAACGACCTACGCATGCTGCTCAACGTCAGTGACGCCAAGGGGCGTA
ACCTCACACTAGATGAACAGGTGCCTAGCCATCTGGGGCTGCGCGATGGCGACGATTACTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (85-287)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.4	95.42	0.557
pilU	Vibrio cholerae strain A1552	57.759	88.55	0.511
pilU	Acinetobacter baylyi ADP1	55.028	91.094	0.501
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.045	89.567	0.377