Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QAY87_RS23565 Genome accession   NZ_CP121766
Coordinates   4976743..4978446 (+) Length   567 a.a.
NCBI ID   WP_003116249.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 22112     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 4956443..4975911 4976743..4978446 flank 832


Gene organization within MGE regions


Location: 4956443..4978446
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QAY87_RS23415 (QAY87_23410) - 4956443..4957657 (+) 1215 WP_023435052.1 site-specific integrase -
  QAY87_RS23420 (QAY87_23415) - 4957629..4958345 (-) 717 WP_073624880.1 hypothetical protein -
  QAY87_RS23425 (QAY87_23420) - 4958494..4958694 (+) 201 WP_023435053.1 helix-turn-helix domain-containing protein -
  QAY87_RS23430 (QAY87_23425) - 4958691..4959107 (+) 417 WP_023435054.1 hypothetical protein -
  QAY87_RS23435 (QAY87_23430) - 4959100..4959333 (+) 234 WP_023110507.1 hypothetical protein -
  QAY87_RS23440 (QAY87_23435) - 4959330..4959578 (+) 249 WP_023435055.1 hypothetical protein -
  QAY87_RS23445 (QAY87_23440) - 4959575..4959880 (+) 306 WP_023109345.1 hypothetical protein -
  QAY87_RS33330 - 4959883..4960320 (+) 438 WP_305954514.1 hypothetical protein -
  QAY87_RS23455 (QAY87_23450) - 4960359..4960826 (+) 468 WP_031285502.1 hypothetical protein -
  QAY87_RS23460 (QAY87_23455) - 4960833..4961786 (+) 954 WP_306279704.1 DUF3631 domain-containing protein -
  QAY87_RS23465 (QAY87_23460) - 4961786..4962079 (+) 294 WP_023435057.1 hypothetical protein -
  QAY87_RS23470 (QAY87_23465) - 4962135..4963037 (+) 903 WP_023435058.1 hypothetical protein -
  QAY87_RS23475 (QAY87_23470) - 4963961..4964125 (+) 165 WP_153519866.1 hypothetical protein -
  QAY87_RS23480 (QAY87_23475) - 4964142..4965734 (+) 1593 WP_079390841.1 anti-phage dCTP deaminase -
  QAY87_RS23485 (QAY87_23480) - 4966456..4966962 (-) 507 WP_003096122.1 GNAT family N-acetyltransferase -
  QAY87_RS23490 (QAY87_23485) - 4967422..4967664 (-) 243 WP_003096120.1 hypothetical protein -
  QAY87_RS23495 (QAY87_23490) - 4967703..4967984 (+) 282 WP_023435064.1 hypothetical protein -
  QAY87_RS23500 (QAY87_23495) - 4967984..4968220 (+) 237 WP_003096116.1 hypothetical protein -
  QAY87_RS23505 (QAY87_23500) - 4968427..4968708 (-) 282 WP_023435065.1 hypothetical protein -
  QAY87_RS23510 (QAY87_23505) - 4968905..4969603 (+) 699 WP_124014670.1 hypothetical protein -
  QAY87_RS23515 (QAY87_23510) - 4969628..4970632 (+) 1005 WP_031689993.1 pesticin C-terminus-like muramidase -
  QAY87_RS23520 (QAY87_23515) - 4970645..4971307 (+) 663 WP_033992423.1 DUF3218 family protein -
  QAY87_RS23525 (QAY87_23520) fliP 4971587..4972342 (+) 756 WP_031285507.1 flagellar type III secretion system pore protein FliP -
  QAY87_RS23530 (QAY87_23525) fliQ 4972387..4972656 (+) 270 WP_023435365.1 flagellar biosynthesis protein FliQ -
  QAY87_RS23535 (QAY87_23530) fliR 4972656..4973438 (+) 783 Protein_4655 flagellar biosynthetic protein FliR -
  QAY87_RS23540 (QAY87_23535) - 4973441..4973700 (+) 260 Protein_4656 EscU/YscU/HrcU family type III secretion system export apparatus switch protein -
  QAY87_RS23545 (QAY87_23540) - 4974728..4974970 (+) 243 WP_023082027.1 hypothetical protein -
  QAY87_RS23555 (QAY87_23550) - 4975213..4975770 (-) 558 WP_225011032.1 hypothetical protein -
  QAY87_RS23560 (QAY87_23555) - 4975975..4976514 (-) 540 WP_031285592.1 pilin -
  QAY87_RS23565 (QAY87_23560) pilB 4976743..4978446 (+) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62610.54 Da        Isoelectric Point: 5.5603

>NTDB_id=814047 QAY87_RS23565 WP_003116249.1 4976743..4978446(+) (pilB) [Pseudomonas aeruginosa strain 22112]
MNDTIQLSGLARQLVLHELLDEKAAQQAQSQAQRNKLSLVTYLVQSKLVKGQALIELAADQFGIAYCDLNSLERDSLPKD
LISEKLVRQHRVVPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGIIIEKLFENATDSLAGLDDVDLE
GLDVGSGAGASQDDDSSAETDDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPKETLLHEGFP
EDRIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=814047 QAY87_RS23565 WP_003116249.1 4976743..4978446(+) (pilB) [Pseudomonas aeruginosa strain 22112]
ATGAACGACACAATTCAACTCAGCGGCTTGGCTCGCCAGCTCGTCCTGCATGAACTTCTCGATGAAAAGGCCGCCCAACA
GGCCCAATCGCAGGCCCAGCGCAACAAGTTGTCGCTGGTAACCTATCTGGTACAGAGCAAGCTAGTGAAAGGGCAGGCCT
TGATCGAACTGGCTGCGGATCAATTCGGTATCGCCTACTGCGACCTGAACAGCCTTGAGCGGGACAGTCTGCCCAAGGAT
CTGATCAGCGAGAAGCTGGTGCGCCAGCATCGAGTAGTTCCGTTGTGGCGACGCGGCAACAAGCTGTTCGTCGGCATCTC
CGATCCAGCCAACCACCAGGCGATTAACGATGTGCAGTTCAGCACAGGCCTGACTACTGAAGCCATCCTGGTCGAGGATG
ACAAGCTCGGCATCATCATCGAGAAACTATTCGAAAATGCCACTGACAGCCTTGCAGGACTGGACGACGTGGATCTGGAA
GGCCTGGATGTCGGTTCCGGTGCGGGCGCAAGCCAGGATGATGATTCCAGTGCAGAGACAGACGACGCCCCAGTGGTGCG
TTTCGTCAACAAAATGTTGTTGGATGCGATCAGAGGCGGCTCCTCCGACCTGCATTTCGAGCCCTATGAAAAGATTTACC
GCGTACGTTTCCGCACCGATGGCATGCTCCATGAAGTAGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATTTCCGCGCGA
CTCAAGGTGATGGCCGGCCTGGATATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGAC
CAAGTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGTATCCTCGATTCCTCCA
GCGCGCAGATGGGCATCGACGCCCTCGGCTACGAGGAAGACCAGAAGGAACTCTACCTGGCTGCGCTCAAGCAGCCGCAG
GGCATGATCCTGGTCACCGGGCCCACCGGCTCGGGCAAGACGGTCTCCCTGTACACCGGCCTGAACATCCTCAACACCAC
CGACATCAACATCTCCACTGCCGAAGACCCGGTGGAGATCAACTTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCC
AGGGCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGTGAGATTCGCGAC
CTGGAGACGGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
CGAGACCCTGACCCGCCTGCTGAACATGGGCGTGCCAGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGC
GACTTGCGCGAAAACTCTGCTCGCACTGCAAGAAAGAGCATGAGGTACCGAAGGAAACACTCCTCCATGAAGGCTTCCCC
GAGGATCGGATCGGCACCTTCAAGCTGTATTCACCAGTAGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGC
AAGCCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.895

100

0.582
  pilB Acinetobacter baylyi ADP1

57.27

99.471

0.57
  pilB Legionella pneumophila strain ERS1305867

55.106

100

0.552
  pilF Neisseria gonorrhoeae MS11

50.444

99.295

0.501
  pilB Vibrio parahaemolyticus RIMD 2210633

51.948

95.062

0.494
  pilB Vibrio cholerae strain A1552

50.182

97.002

0.487
  pilB Vibrio campbellii strain DS40M4

50.832

95.414

0.485
  pilF Thermus thermophilus HB27

38.584

99.647

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.189

93.474

0.376