Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   Thiowin_RS22645 Genome accession   NZ_CP121472
Coordinates   4893588..4894088 (-) Length   166 a.a.
NCBI ID   WP_328985227.1    Uniprot ID   -
Organism   Thiorhodovibrio winogradskyi strain DSM 6702     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4888588..4899088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Thiowin_RS22615 (Thiowin_04611) - 4889872..4890477 (-) 606 WP_328985223.1 hypothetical protein -
  Thiowin_RS22620 (Thiowin_04612) - 4890490..4890953 (-) 464 Protein_4499 CbbQ/NirQ/NorQ C-terminal domain-containing protein -
  Thiowin_RS22625 (Thiowin_04614) - 4890950..4891819 (-) 870 WP_328985224.1 Rpn family recombination-promoting nuclease/putative transposase -
  Thiowin_RS22630 - 4892123..4892302 (-) 180 Protein_4501 DUF2887 domain-containing protein -
  Thiowin_RS22635 (Thiowin_04616) - 4892871..4893059 (-) 189 WP_328985225.1 hypothetical protein -
  Thiowin_RS22640 (Thiowin_04617) - 4893062..4893373 (-) 312 WP_328985226.1 type II toxin-antitoxin system RelE/ParE family toxin -
  Thiowin_RS22645 pilA 4893588..4894088 (-) 501 WP_328985227.1 pilin Machinery gene
  Thiowin_RS22650 (Thiowin_04618) - 4894443..4894742 (+) 300 WP_328985228.1 DUF6516 family protein -
  Thiowin_RS22655 (Thiowin_04619) - 4894712..4895080 (+) 369 WP_328985229.1 MarR family transcriptional regulator -
  Thiowin_RS25525 - 4895106..4895270 (+) 165 WP_408034248.1 PIN domain-containing protein -
  Thiowin_RS22660 (Thiowin_04620) - 4895267..4895512 (+) 246 WP_328985230.1 toxin-antitoxin system HicB family antitoxin -
  Thiowin_RS25530 - 4895662..4895796 (+) 135 WP_408034126.1 hypothetical protein -
  Thiowin_RS22670 (Thiowin_04623) - 4896138..4896467 (+) 330 WP_328985232.1 hypothetical protein -
  Thiowin_RS22675 (Thiowin_04624) - 4896666..4898918 (-) 2253 WP_328985233.1 VWA domain-containing protein -

Sequence

Protein


Download         Length: 166 a.a.        Molecular weight: 16414.68 Da        Isoelectric Point: 6.9430

>NTDB_id=812331 Thiowin_RS22645 WP_328985227.1 4893588..4894088(-) (pilA) [Thiorhodovibrio winogradskyi strain DSM 6702]
MKNHQQGFTLIELMIVVAIIGILAAIALPAYQDYMVRARVSEGLAAASAAKVTVADNLASGNPRAAATGYAQGYTSPTAT
ENVTSVAIDATTGAITVTMTAAAGNGTLILTPNAPTGTALPVGTAAFTPPNDSVAWRCGAAGADAAGFAGFAAGTLPARF
APSSCK

Nucleotide


Download         Length: 501 bp        

>NTDB_id=812331 Thiowin_RS22645 WP_328985227.1 4893588..4894088(-) (pilA) [Thiorhodovibrio winogradskyi strain DSM 6702]
ATGAAGAATCATCAACAAGGTTTTACATTGATCGAACTCATGATCGTGGTGGCGATCATTGGTATTTTGGCGGCGATTGC
TTTGCCGGCTTATCAGGATTACATGGTTAGAGCGCGTGTGAGCGAGGGTTTGGCTGCAGCTTCTGCAGCAAAAGTCACAG
TTGCTGATAATTTAGCATCTGGAAATCCAAGAGCCGCAGCGACCGGATATGCCCAAGGCTACACAAGTCCTACCGCTACA
GAAAATGTAACTAGCGTTGCAATCGATGCTACTACTGGCGCGATCACTGTTACCATGACTGCTGCTGCTGGCAACGGCAC
GCTTATCTTGACGCCCAATGCACCAACTGGCACAGCTCTCCCAGTCGGTACTGCGGCATTTACGCCTCCAAACGATTCAG
TAGCTTGGCGTTGTGGTGCTGCTGGTGCTGACGCCGCAGGTTTCGCAGGCTTTGCAGCAGGTACCTTGCCCGCAAGGTTT
GCGCCTTCATCGTGCAAGTAG

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-166)

Prokaryotic N-terminal methylation motif (N_methyl)

(2-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

49.412

100

0.506
  pilA2 Legionella pneumophila strain ERS1305867

49.08

98.193

0.482
  pilA2 Legionella pneumophila str. Paris

48.466

98.193

0.476
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.974

100

0.458
  comP Acinetobacter baylyi ADP1

40.805

100

0.428