Detailed information NTG0081177

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OCUAC_RS13620
• Genome accession: NZ_AP023077
• Coordinates: 2851703..2852821 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain OCU_Ac16a
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2846703..2857821

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OCUAC_RS13610	-	2849819..2850511 (-)	693	WP_001108503.1	YggS family pyridoxal phosphate-dependent enzyme	-
OCUAC_RS13615	pilT	2850638..2851675 (+)	1038	WP_072689322.1	type IV pilus twitching motility protein PilT	Machinery gene
OCUAC_RS13620	pilU	2851703..2852821 (+)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OCUAC_RS13625	fur	2852891..2853328 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
OCUAC_RS13630	-	2853440..2853838 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
OCUAC_RS13635	-	2853893..2854216 (-)	324	WP_000442589.1	RnfH family protein	-
OCUAC_RS13640	-	2854216..2855283 (-)	1068	WP_029749783.1	membrane protein	-
OCUAC_RS13645	-	2855436..2855891 (-)	456	WP_000782976.1	bacteriohemerythrin	-
OCUAC_RS13650	-	2856144..2856992 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=81177 OCUAC_RS13620 WP_000347034.1 2851703..2852821(+) (pilU) [Acinetobacter baumannii strain OCU_Ac16a]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=81177 OCUAC_RS13620 WP_000347034.1 2851703..2852821(+) (pilU) [Acinetobacter baumannii strain OCU_Ac16a]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTCTTTATTACAGATGGCGTTGCGCCTTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGTGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTAAGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAATAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39