Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   P8T11_RS20510 Genome accession   NZ_CP121261
Coordinates   4584642..4586087 (+) Length   481 a.a.
NCBI ID   WP_268080308.1    Uniprot ID   -
Organism   Achromobacter spanius strain LIG8     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4579642..4591087
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P8T11_RS20495 (P8T11_20495) - 4580455..4581783 (+) 1329 WP_268080311.1 amidase -
  P8T11_RS20500 (P8T11_20500) leuA 4581939..4583651 (-) 1713 WP_268080310.1 2-isopropylmalate synthase -
  P8T11_RS20505 (P8T11_20505) - 4584011..4584643 (-) 633 WP_268080309.1 hypothetical protein -
  P8T11_RS20510 (P8T11_20510) rpoN/rpoN1 4584642..4586087 (+) 1446 WP_268080308.1 RNA polymerase factor sigma-54 Machinery gene
  P8T11_RS20515 (P8T11_20515) - 4586209..4586490 (+) 282 WP_050448875.1 (2Fe-2S)-binding protein -
  P8T11_RS20520 (P8T11_20520) bfr 4586955..4587431 (+) 477 WP_050448876.1 bacterioferritin -
  P8T11_RS20525 (P8T11_20525) - 4587506..4588192 (-) 687 WP_268080307.1 hypothetical protein -
  P8T11_RS20530 (P8T11_20530) - 4588217..4589823 (-) 1607 Protein_4067 peptide chain release factor 3 -
  P8T11_RS20535 (P8T11_20535) - 4589937..4590566 (-) 630 WP_268080305.1 LysE family transporter -

Sequence

Protein


Download         Length: 481 a.a.        Molecular weight: 52568.90 Da        Isoelectric Point: 4.8503

>NTDB_id=811413 P8T11_RS20510 WP_268080308.1 4584642..4586087(+) (rpoN/rpoN1) [Achromobacter spanius strain LIG8]
MQNIVPMTRPILELRPGQHLTLTPQLQQSIRLLQLSTLDLEAEISQVLADNPLLEREDDTPAAEAQAESERESSVQDDEP
AVERETPVDEMPGSGGVYSDDDSGLPEAARPDTLREHLLGQLMLTRAAPRDAALAALLIDELDENGYLGSPLEEILTWLP
ADMEPDLDELRAALSLLQSFDPPGIGARDMADCLVLQLRHPDLSRLPEAADGAVLACARVICAQHLPVLATGNAARLCAA
VGCDEATFRAAHSLILRLEPRPGRAWTVPAADYAVPDVIVRKTRRGWQVTLNSAAVPRLQINGLYAQMLGNQKESAHAGL
QTQLQQARWMIRNVEQRFDTILRVAQAIVERQTAFFSQGPSAMRPLILKDIAGELGLHESTISRATTQKYMLTPFGTLEL
KRFFGTGVSTDGGDATSATAVQTFIRQMVAEENRAKPLSDSQIMQKLADQGIVIARRTVAKYREALRIAPATLRKAQASA
R

Nucleotide


Download         Length: 1446 bp        

>NTDB_id=811413 P8T11_RS20510 WP_268080308.1 4584642..4586087(+) (rpoN/rpoN1) [Achromobacter spanius strain LIG8]
ATGCAAAATATTGTACCTATGACCCGTCCAATTTTAGAACTGCGACCTGGCCAGCATTTGACGCTGACCCCTCAACTGCA
GCAATCGATACGATTGTTGCAGTTGTCCACGCTCGACCTTGAGGCCGAGATCTCGCAAGTGCTGGCGGACAACCCGCTGC
TGGAGCGTGAAGACGACACCCCCGCCGCCGAGGCGCAAGCCGAATCCGAGCGCGAATCCTCCGTGCAGGACGATGAGCCC
GCGGTAGAGCGCGAGACGCCAGTGGACGAAATGCCCGGCTCGGGCGGGGTCTATTCGGATGATGATTCCGGCCTGCCCGA
GGCGGCGCGCCCCGACACCCTGCGCGAGCATCTGCTGGGCCAATTGATGTTGACTCGGGCCGCCCCGCGTGACGCCGCGC
TGGCTGCGCTGCTGATCGACGAACTGGACGAAAACGGGTATCTGGGGTCGCCGCTAGAGGAAATCCTGACGTGGCTGCCC
GCCGACATGGAGCCGGACCTGGACGAATTGCGCGCTGCGCTGTCCTTGTTGCAGTCGTTCGACCCGCCTGGCATCGGTGC
GCGAGACATGGCCGACTGCCTGGTGTTGCAATTGCGCCATCCCGACCTGTCCCGGTTGCCGGAAGCGGCTGACGGGGCGG
TTTTAGCGTGCGCCCGGGTTATCTGCGCGCAGCATCTGCCCGTACTGGCCACGGGTAACGCCGCTCGCCTTTGCGCCGCC
GTGGGCTGTGACGAGGCGACCTTTCGTGCTGCCCATTCGCTGATCCTGCGGTTGGAACCTCGCCCCGGACGGGCCTGGAC
GGTGCCGGCGGCTGATTACGCCGTGCCGGACGTCATCGTGCGCAAGACCCGGCGCGGGTGGCAAGTCACGCTCAACAGCG
CTGCCGTGCCCCGGTTGCAGATCAACGGCCTGTATGCGCAGATGCTGGGCAACCAGAAGGAATCCGCGCACGCGGGCCTG
CAGACCCAATTGCAGCAAGCCAGATGGATGATCCGCAACGTGGAGCAGCGCTTTGACACGATCCTGCGGGTCGCCCAGGC
GATCGTGGAACGCCAGACCGCGTTCTTCAGCCAGGGGCCGTCAGCTATGCGCCCGTTGATCCTGAAAGACATTGCGGGCG
AGCTCGGATTGCACGAATCGACCATTTCACGGGCTACAACACAGAAGTACATGCTCACTCCGTTCGGCACGCTGGAGCTC
AAGCGCTTCTTCGGCACGGGCGTCTCCACGGACGGCGGCGATGCAACGTCCGCCACGGCGGTGCAGACGTTCATCCGCCA
AATGGTGGCTGAAGAGAACCGTGCCAAGCCCTTGTCCGATAGCCAGATCATGCAGAAACTGGCCGATCAGGGGATAGTCA
TCGCCCGTCGGACGGTGGCAAAGTACCGCGAAGCGCTGCGGATTGCCCCCGCCACGCTGCGCAAGGCACAGGCTAGCGCG
CGTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(108-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(320-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

48.713

100

0.511