Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   RS172_RS03820 Genome accession   NZ_CP135646
Coordinates   785894..787387 (+) Length   497 a.a.
NCBI ID   WP_033547624.1    Uniprot ID   -
Organism   Escherichia coli strain TUM14668     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 780894..792387
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS172_RS03800 (RS172_03800) pilD 781542..782351 (+) 810 WP_000895866.1 prepilin peptidase PppA Machinery gene
  RS172_RS03805 (RS172_03805) gspS2 782417..782827 (+) 411 WP_001298257.1 type II secretion system pilot lipoprotein GspS-beta -
  RS172_RS03810 (RS172_03810) gspC 782845..783804 (+) 960 WP_000135085.1 type II secretion system protein GspC -
  RS172_RS03815 (RS172_03815) gspD 783834..785894 (+) 2061 WP_033560308.1 type II secretion system secretin GspD -
  RS172_RS03820 (RS172_03820) pilB/pilB1 785894..787387 (+) 1494 WP_033547624.1 type II secretion system ATPase GspE Machinery gene
  RS172_RS03825 (RS172_03825) gspF 787387..788610 (+) 1224 WP_001173427.1 type II secretion system inner membrane protein GspF -
  RS172_RS03830 (RS172_03830) gspG 788627..789082 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  RS172_RS03835 (RS172_03835) gspH 789086..789649 (+) 564 WP_001115150.1 type II secretion system minor pseudopilin GspH -
  RS172_RS03840 (RS172_03840) gspI 789646..790017 (+) 372 WP_000820119.1 type II secretion system minor pseudopilin GspI -
  RS172_RS03845 (RS172_03845) gspJ 790014..790619 (+) 606 WP_011579195.1 type II secretion system minor pseudopilin GspJ -
  RS172_RS03850 (RS172_03850) gspK 790616..791593 (+) 978 WP_000631647.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55154.29 Da        Isoelectric Point: 5.9081

>NTDB_id=810940 RS172_RS03820 WP_033547624.1 785894..787387(+) (pilB/pilB1) [Escherichia coli strain TUM14668]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNASFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSLELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETALDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=810940 RS172_RS03820 WP_033547624.1 785894..787387(+) (pilB/pilB1) [Escherichia coli strain TUM14668]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGTCTTTTACGCTGAGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAGCTGATGGAAGATCTCGGTTCTGCTGAGGACTTTTTTACTCTCGCAGAAGAACTACCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCAATCATCAAACTGATCAACGCCATGTTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACACTACATGAAATGCTGCGT
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATTAAGGTGATGGCACGGCTGGATATCGCCGAAAAACGCGTACC
GCAGGATGGGCGCATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCCGCCTGGGGGG
AACGGGTGGTGCTGCGACTGCTGGACAAAAACCAGGCTCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCTCGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACCGGTTCCGGCAAAAGCACCAC
GCTGTATGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTTGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGATTGCCGCCAGTCCGCGCCTGCC
ACCGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCGAACCGTCACGCTGTACCATCCGCAGGGCTGTCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCGCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Legionella pneumophila strain ERS1305867

42.398

93.964

0.398
  pilB Vibrio parahaemolyticus RIMD 2210633

47.859

79.879

0.382
  pilB Vibrio campbellii strain DS40M4

46.97

79.678

0.374
  pilB Acinetobacter baumannii D1279779

46.115

80.282

0.37
  pilB Vibrio cholerae strain A1552

45.707

79.678

0.364
  pilB Acinetobacter baylyi ADP1

46.154

78.471

0.362
  pilF Thermus thermophilus HB27

45

80.483

0.362