Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   RO838_RS09625 Genome accession   NZ_CP135134
Coordinates   2080861..2081919 (-) Length   352 a.a.
NCBI ID   WP_004089911.1    Uniprot ID   B2I8B6
Organism   Xylella fastidiosa strain CFBP8478     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2075861..2086919
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RO838_RS09605 (RO838_09605) comQ/pilQ 2077091..2079004 (-) 1914 WP_057682736.1 type IV pilus secretin PilQ Machinery gene
  RO838_RS09610 (RO838_09610) - 2079004..2079534 (-) 531 WP_057682735.1 pilus assembly protein PilP -
  RO838_RS09615 (RO838_09615) - 2079531..2080199 (-) 669 WP_004089909.1 type 4a pilus biogenesis protein PilO -
  RO838_RS09620 (RO838_09620) - 2080283..2080861 (-) 579 Protein_1879 PilN domain-containing protein -
  RO838_RS09625 (RO838_09625) pilM 2080861..2081919 (-) 1059 WP_004089911.1 type IV pilus biogenesis protein PilM Machinery gene
  RO838_RS09630 (RO838_09630) - 2082159..2084588 (+) 2430 WP_012382725.1 penicillin-binding protein 1A -
  RO838_RS09635 (RO838_09635) - 2084892..2085857 (-) 966 WP_004086600.1 aldo/keto reductase -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37539.19 Da        Isoelectric Point: 4.6541

>NTDB_id=810199 RO838_RS09625 WP_004089911.1 2080861..2081919(-) (pilM) [Xylella fastidiosa strain CFBP8478]
MGLFSKKQSVLVGVDISSTAVKLLQLSRSGNRFKVEHYAVEPLPLNAVAEKGIVEVEQVGEAIRRAVSRSGTKAKFAAAA
VAGSAVITKLIPMPAGLDEQDLEAQIEIEATNYIPYPIEEVSLDFEVLGPVPNNTEMVQVLLAASRSENVELRQSALELG
GLTAKVIDVEALAVENAFSLIAQELSVGSNALVALIDIGATMSTLNVLHSGRSLYAREQLFGGKQLTDEVMHRYGMTYEE
AGQAKRQGGLPQSYEVEVLGPFKDSVIQQISRLLQFFYAGSEYNRVDCIVLAGGCAVIAGLPAMVEERLGVVTVVANPLA
QMTLGAKVQAHTLAQDAPALMIATGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=810199 RO838_RS09625 WP_004089911.1 2080861..2081919(-) (pilM) [Xylella fastidiosa strain CFBP8478]
GTGGGGCTTTTTTCAAAAAAACAGTCGGTATTGGTTGGTGTCGACATTAGTTCAACTGCAGTGAAGCTCTTACAGCTTTC
CCGCAGTGGTAATCGCTTCAAGGTGGAACACTATGCTGTGGAGCCGCTACCTCTGAATGCGGTCGCCGAGAAGGGCATCG
TTGAAGTCGAGCAGGTTGGTGAAGCAATCCGTCGTGCGGTAAGTCGTTCTGGAACTAAGGCCAAATTTGCCGCTGCCGCG
GTTGCCGGTTCGGCAGTGATCACCAAGTTGATACCGATGCCTGCTGGTCTGGACGAGCAGGATTTGGAAGCTCAGATAGA
AATCGAGGCTACGAATTACATCCCGTACCCTATTGAAGAGGTCAGCCTAGATTTCGAGGTGCTTGGTCCGGTTCCCAACA
ACACGGAGATGGTCCAAGTTTTGCTGGCTGCGTCTCGTTCGGAGAATGTCGAATTGCGCCAATCTGCGTTGGAGCTGGGT
GGGTTGACTGCCAAGGTTATTGACGTGGAAGCTTTAGCGGTTGAGAACGCTTTCTCTCTTATTGCCCAAGAACTGTCGGT
TGGGAGTAATGCATTGGTTGCATTGATCGACATCGGTGCAACGATGTCGACGCTTAATGTGCTGCATAGTGGTCGCAGTT
TGTACGCGCGCGAGCAGCTATTTGGTGGTAAGCAGCTTACTGACGAAGTGATGCATCGCTATGGGATGACTTACGAGGAG
GCTGGTCAGGCCAAGCGTCAAGGAGGTTTGCCGCAAAGCTATGAGGTTGAAGTGCTGGGACCATTTAAGGACTCTGTGAT
CCAGCAGATCAGCCGTTTATTACAGTTTTTCTATGCTGGCAGTGAGTACAACCGAGTTGATTGCATCGTGTTGGCGGGAG
GCTGTGCTGTGATTGCGGGGTTACCGGCTATGGTCGAAGAACGGTTGGGTGTCGTTACCGTTGTGGCTAATCCGCTTGCG
CAGATGACTTTAGGGGCGAAAGTGCAAGCACACACGCTTGCTCAAGATGCACCTGCTTTAATGATTGCTACTGGTCTGGC
TTTGAGGAGTTTTGACTGA

Domains

Predicted by InterProScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B2I8B6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

48.864

100

0.489
  comM Acinetobacter nosocomialis M2

48.864

100

0.489
  comM/pilM Acinetobacter baylyi ADP1

48.011

100

0.48
  pilM Legionella pneumophila strain ERS1305867

45.609

100

0.457