Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   OO986_RS05445 Genome accession   NZ_AP026552
Coordinates   1182355..1183359 (+) Length   334 a.a.
NCBI ID   WP_011080039.1    Uniprot ID   A0A3Q0L4Y8
Organism   Vibrio vulnificus strain VV20-8B-2 isolate homogenised shrimp     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1177355..1188359
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OO986_RS05425 (VV208B2_10060) aspS 1177707..1179485 (+) 1779 WP_011080035.1 aspartate--tRNA ligase -
  OO986_RS05430 (VV208B2_10070) ruvC 1179552..1180073 (+) 522 WP_011080036.1 crossover junction endodeoxyribonuclease RuvC -
  OO986_RS05435 (VV208B2_10080) - 1180188..1181648 (+) 1461 WP_011080037.1 methyl-accepting chemotaxis protein -
  OO986_RS05440 (VV208B2_10090) ruvA 1181719..1182333 (+) 615 WP_011080038.1 Holliday junction branch migration protein RuvA -
  OO986_RS05445 (VV208B2_10100) ruvB 1182355..1183359 (+) 1005 WP_011080039.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  OO986_RS05450 (VV208B2_10110) cydA 1183822..1185408 (+) 1587 WP_013571418.1 cytochrome ubiquinol oxidase subunit I -
  OO986_RS05455 (VV208B2_10120) cydB 1185425..1186561 (+) 1137 WP_011080041.1 cytochrome d ubiquinol oxidase subunit II -
  OO986_RS05460 cydX 1186574..1186681 (+) 108 WP_000270284.1 cytochrome bd-I oxidase subunit CydX -
  OO986_RS05465 (VV208B2_10130) ybgE 1186674..1186976 (+) 303 WP_017421971.1 cyd operon protein YbgE -
  OO986_RS05470 (VV208B2_10140) ybgC 1187121..1187540 (+) 420 WP_108694371.1 tol-pal system-associated acyl-CoA thioesterase -
  OO986_RS05475 (VV208B2_10150) tolQ 1187530..1188213 (+) 684 WP_108694372.1 protein TolQ -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 37126.56 Da        Isoelectric Point: 4.8134

>NTDB_id=80855 OO986_RS05445 WP_011080039.1 1182355..1183359(+) (ruvB) [Vibrio vulnificus strain VV20-8B-2 isolate homogenised shrimp]
MIEADRLIAPDNPAFRDEDVIDRAIRPKKLADYQGQDHVRDQMEIFIKAAQLRNEALDHLLIFGPPGLGKTTLANIVANE
MGVNIRTTSGPVLEKAGDLAALLTNLEENDVLFIDEIHRLSPMVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLI
GATTRAGSLTSPLRDRFGITQRLEYYKIPDLQNIVQRSADCLGLSMEAEGALEVARRARGTPRIANRLLRRVRDFAEVKG
NGHICADTADKALNMLDVDSKGFDYMDRKLLLAIMEKFGGGPVGLDNMAAAIGEEKDTIEDVLEPYLIQQGYLQRTPRGR
IATDRAYLHFGIEK

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=80855 OO986_RS05445 WP_011080039.1 1182355..1183359(+) (ruvB) [Vibrio vulnificus strain VV20-8B-2 isolate homogenised shrimp]
ATGATTGAAGCAGATCGCTTAATTGCTCCGGACAATCCCGCTTTTCGTGATGAAGATGTGATTGACCGCGCGATACGACC
AAAGAAACTCGCCGATTACCAAGGCCAAGATCACGTACGTGATCAAATGGAAATTTTTATTAAAGCCGCGCAATTGCGTA
ACGAAGCGCTCGACCATTTGTTGATTTTTGGCCCTCCAGGTTTGGGTAAAACCACCTTAGCGAACATTGTTGCAAACGAG
ATGGGCGTAAATATTCGCACCACGTCAGGACCGGTGTTAGAAAAAGCGGGAGATTTAGCGGCCTTGCTGACCAATCTTGA
AGAAAACGACGTGCTCTTCATTGATGAAATACACCGACTCAGCCCGATGGTGGAAGAAGTGTTGTATCCAGCCATGGAAG
ATTACCAATTGGATATTATGATTGGTGAAGGCCCTGCTGCACGTTCAATCAAAATCGATTTGCCGCCCTTTACGCTAATT
GGTGCAACCACTCGTGCCGGCTCACTGACTTCCCCTCTGCGTGATCGTTTTGGTATTACCCAGCGTTTGGAGTATTACAA
AATTCCTGATCTGCAGAATATCGTGCAACGCAGTGCTGACTGCTTGGGGCTTTCCATGGAAGCGGAAGGGGCATTAGAGG
TGGCGCGTCGTGCGCGTGGTACACCGCGTATTGCCAATCGCTTGTTGCGCCGTGTACGCGATTTTGCTGAAGTAAAAGGC
AATGGTCACATCTGTGCAGACACCGCAGACAAAGCGCTCAATATGCTCGATGTGGACTCGAAAGGTTTTGATTACATGGA
CCGCAAGTTGCTGTTGGCCATCATGGAGAAATTTGGTGGTGGTCCGGTTGGCTTGGATAACATGGCAGCCGCGATCGGTG
AAGAGAAAGACACGATTGAAGATGTGTTAGAGCCATATTTGATTCAGCAGGGTTATTTACAACGCACGCCTCGAGGGCGA
ATCGCAACCGATCGGGCGTATCTTCATTTCGGAATTGAAAAGTAA

Domains

Predicted by InterProScan.

RuvB C-terminal winged helix domain (WHD_RuvB)

(261-330)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(25-183)

RuvB AAA lid domain (AAA_lid_4)

(186-259)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L4Y8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.79

98.503

0.599
  ruvB Streptococcus pneumoniae TIGR4

59.752

96.707

0.578
  ruvB Streptococcus pneumoniae R6

59.752

96.707

0.578
  ruvB Streptococcus pneumoniae D39

59.752

96.707

0.578
  ruvB Synechocystis sp. PCC 6803

55.016

92.515

0.509
  ruvB Helicobacter pylori 26695

54.276

91.018

0.494

Multiple sequence alignment