Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   RMO93_RS02540 Genome accession   NZ_CP134805
Coordinates   554912..556150 (+) Length   412 a.a.
NCBI ID   WP_311820221.1    Uniprot ID   -
Organism   Escherichia coli strain E7-1N7     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 549912..561150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RMO93_RS02515 (RMO93_02515) mrcA 550192..552744 (-) 2553 WP_001562196.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  RMO93_RS02520 (RMO93_02520) hofM 552864..553643 (+) 780 WP_311820217.1 DNA utilization protein HofM -
  RMO93_RS02525 (RMO93_02525) hofN 553643..554182 (+) 540 WP_311820218.1 DNA utilization protein HofN -
  RMO93_RS02530 (RMO93_02530) hofO 554166..554606 (+) 441 WP_311820219.1 DNA utilization protein HofO -
  RMO93_RS02535 (RMO93_02535) hofP 554596..555000 (+) 405 WP_311820220.1 DNA utilization protein HofP -
  RMO93_RS02540 (RMO93_02540) comE 554912..556150 (+) 1239 WP_311820221.1 DNA uptake porin HofQ Machinery gene
  RMO93_RS02545 (RMO93_02545) aroK 556551..557072 (+) 522 WP_000818618.1 shikimate kinase AroK -
  RMO93_RS02550 (RMO93_02550) aroB 557129..558217 (+) 1089 WP_000439850.1 3-dehydroquinate synthase -
  RMO93_RS02555 (RMO93_02555) damX 558309..559595 (+) 1287 WP_311820222.1 cell division protein DamX -
  RMO93_RS02560 (RMO93_02560) dam 559702..560538 (+) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44779.29 Da        Isoelectric Point: 5.9488

>NTDB_id=808121 RMO93_RS02540 WP_311820221.1 554912..556150(+) (comE) [Escherichia coli strain E7-1N7]
MKQWIAALLLMLIPSVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSIAWQNDNIARQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTTLGSDLSVATATTHIGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=808121 RMO93_RS02540 WP_311820221.1 554912..556150(+) (comE) [Escherichia coli strain E7-1N7]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCAGCGTACAGGCGGCAAAGCCGCAAAAAGTGACACTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGCCAGGAGGGCAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATGACAATATCGCCCGTCAGGAAGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA
AAGCTGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAACGGCGTTAAGCGCCCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTCTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGTGTGAAATGGACGCTGGCCGATGCGCAACAAGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATATCGGTTTTAACATTGGACGCATCAAC
GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAAGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCAGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.385

100

0.396
  pilQ Vibrio campbellii strain DS40M4

38.48

100

0.393
  comE Haemophilus influenzae 86-028NP

37.156

100

0.393
  pilQ Vibrio cholerae strain A1552

38.015

100

0.381
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

38.015

100

0.381
  pilQ Pseudomonas aeruginosa PAK

34.404

100

0.364
  comE Glaesserella parasuis strain SC1401

35.476

100

0.362