Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   RJ646_RS03965 Genome accession   NZ_CP134358
Coordinates   821852..823345 (+) Length   497 a.a.
NCBI ID   WP_000249346.1    Uniprot ID   -
Organism   Escherichia coli strain TUM13936     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 816852..828345
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJ646_RS03945 (RJ646_03945) pilD 817500..818309 (+) 810 WP_000895883.1 prepilin peptidase PppA Machinery gene
  RJ646_RS03950 (RJ646_03950) gspS2 818375..818785 (+) 411 WP_038339820.1 type II secretion system pilot lipoprotein GspS-beta -
  RJ646_RS03955 (RJ646_03955) gspC 818803..819762 (+) 960 WP_053273597.1 type II secretion system protein GspC -
  RJ646_RS03960 (RJ646_03960) gspD 819792..821852 (+) 2061 WP_000498813.1 type II secretion system secretin GspD -
  RJ646_RS03965 (RJ646_03965) pilB/pilB1 821852..823345 (+) 1494 WP_000249346.1 type II secretion system ATPase GspE Machinery gene
  RJ646_RS03970 (RJ646_03970) gspF 823345..824568 (+) 1224 WP_276270154.1 type II secretion system inner membrane protein GspF -
  RJ646_RS03975 (RJ646_03975) gspG 824585..825040 (+) 456 WP_001087290.1 type II secretion system major pseudopilin GspG -
  RJ646_RS03980 (RJ646_03980) gspH 825044..825607 (+) 564 WP_001115142.1 type II secretion system minor pseudopilin GspH -
  RJ646_RS03985 (RJ646_03985) gspI 825604..825975 (+) 372 WP_000820074.1 type II secretion system minor pseudopilin GspI -
  RJ646_RS03990 (RJ646_03990) gspJ 825972..826577 (+) 606 WP_001254779.1 type II secretion system minor pseudopilin GspJ -
  RJ646_RS03995 (RJ646_03995) gspK 826574..827551 (+) 978 WP_276270155.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55178.32 Da        Isoelectric Point: 6.0390

>NTDB_id=802825 RJ646_RS03965 WP_000249346.1 821852..823345(+) (pilB/pilB1) [Escherichia coli strain TUM13936]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQAAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=802825 RJ646_RS03965 WP_000249346.1 821852..823345(+) (pilB/pilB1) [Escherichia coli strain TUM13936]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTGCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGACTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAGCTAATGGAAGATCTCGGTTCTGCTGAGGACTTTTTTACTCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATTCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGCGTTGACGGCACATTACATGAAATGCTGCGC
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATCAAGGTGATGGCACGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGACGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCCTCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTTAGCCAGCAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGTAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTCCAGGCTTCACTTACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGGGTAGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGTACGCTGTGCCCCGACTGCCGCCAGGCCGCGCCAGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGATGCGCGAACCGTCACTCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCATAAAGGTTTCCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGCATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Acinetobacter baumannii D1279779

46.366

80.282

0.372
  pilB Vibrio cholerae strain A1552

46.465

79.678

0.37
  pilB Acinetobacter baylyi ADP1

46.154

78.471

0.362
  pilF Thermus thermophilus HB27

45

80.483

0.362