Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RHT80_RS01975 Genome accession   NZ_CP133913
Coordinates   420430..421911 (-) Length   493 a.a.
NCBI ID   WP_134778498.1    Uniprot ID   -
Organism   Escherichia coli strain M23917     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 415430..426911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHT80_RS01945 (RHT80_01940) gspK 416343..417326 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  RHT80_RS01950 (RHT80_01945) gspJ 417319..417906 (-) 588 WP_000610636.1 type II secretion system minor pseudopilin GspJ -
  RHT80_RS01955 (RHT80_01950) gspI 417899..418276 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  RHT80_RS01960 (RHT80_01955) gspH 418273..418782 (-) 510 WP_134778497.1 type II secretion system minor pseudopilin GspH -
  RHT80_RS01965 (RHT80_01960) gspG 418790..419227 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  RHT80_RS01970 (RHT80_01965) gspF 419237..420433 (-) 1197 WP_001109573.1 type II secretion system inner membrane protein GspF -
  RHT80_RS01975 (RHT80_01970) pilB 420430..421911 (-) 1482 WP_134778498.1 type II secretion system ATPase GspE Machinery gene
  RHT80_RS01980 (RHT80_01975) gspD 421921..423873 (-) 1953 WP_016236367.1 type II secretion system secretin GspD -
  RHT80_RS01985 (RHT80_01980) gspC 423857..424671 (-) 815 Protein_394 type II secretion system protein GspC -
  RHT80_RS01990 (RHT80_01985) gspA 424851..426320 (+) 1470 WP_087897931.1 type II secretion system protein GspA -
  RHT80_RS01995 (RHT80_01990) gspB 426322..426741 (+) 420 WP_000461449.1 putative general secretion pathway protein GspB -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54640.59 Da        Isoelectric Point: 6.2412

>NTDB_id=801466 RHT80_RS01975 WP_134778498.1 420430..421911(-) (pilB) [Escherichia coli strain M23917]
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETTQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFK
YHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHKALIKNGLQKVISGDTSWDE
VMRVASATLESEA

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=801466 RHT80_RS01975 WP_134778498.1 420430..421911(-) (pilB) [Escherichia coli strain M23917]
ATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTAT
TTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGTCAGTGCCAGACCATGACCAGTTTGT
CACAGGCTGATTTCGAAGCCCGGCTGGAAGCGGTGTTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATC
GATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC
GGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAA
AAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTT
ATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTAT
CGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATA
AAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAGCAGGATTTAGAAAATCTCATTCAGCTT
CCGCACGGTATTATCCTGGTGACAGGGCCGACAGGCTCCGGTAAAAGCACCACGCTCTACGCCATCCTTTCGGCGCTGAA
TACTCCCGGCCGCAATATTCTGACGGTAGAAGATCCCGTGGAATATGAGCTGGAAGGCATTGGGCAAACGCAGGTGAATA
CCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAAATT
CGTGATACAGAAACCACGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAG
TGCATCAGGCGCAGTGACCCGGCTCCGCGACATGGGCGTCGAATCATTCCTGCTTTCGTCTTCCCTGGCAGGGATTATCG
CGCAACGTCTGGTTCGTCGCCTGTGTCCGCAATGCCGACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAA
TATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCTGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCAT
GGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCATGAAAATGTGGATGAACAAGCACTGGAGC
GACTAGTCCGGCAACAACACAAGGCCTTAATCAAAAATGGCCTGCAAAAAGTGATAAGCGGTGACACCTCCTGGGATGAG
GTTATGCGCGTCGCCAGTGCCACGCTGGAGAGCGAAGCATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(114-380)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

46.453

88.641

0.412
  pilB Vibrio cholerae strain A1552

48.201

84.584

0.408
  pilB Acinetobacter baumannii D1279779

45.351

89.452

0.406
  pilB Legionella pneumophila strain ERS1305867

41.164

97.566

0.402
  pilB Vibrio parahaemolyticus RIMD 2210633

46.118

86.207

0.398
  pilB/pilB1 Synechocystis sp. PCC 6803

40.966

96.552

0.396
  pilB Acinetobacter baylyi ADP1

48.082

79.31

0.381
  pilF Thermus thermophilus HB27

45.063

80.122

0.361