Detailed information    

insolico Bioinformatically predicted

Overview

Name   htrA   Type   Regulator
Locus tag   RFN65_RS00425 Genome accession   NZ_CP133703
Coordinates   73093..74295 (+) Length   400 a.a.
NCBI ID   WP_021481077.1    Uniprot ID   -
Organism   Bacillus subtilis strain CP35     
Function   require for competence development (predicted from homology)   
Competence regulation

Genomic Context

Location: 68093..79295
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN65_RS00405 (RFN65_00405) walK 68174..70009 (+) 1836 WP_009968432.1 cell wall metabolism sensor histidine kinase WalK -
  RFN65_RS00410 (RFN65_00410) walH 69999..71366 (+) 1368 WP_063335952.1 WalRK two-component regulatory system regulator WalH -
  RFN65_RS00415 (RFN65_00415) walI 71353..72195 (+) 843 WP_003244037.1 WalRK two-component regulatory system regulator WalI -
  RFN65_RS00420 (RFN65_00420) vicX 72217..73011 (+) 795 WP_015483958.1 MBL fold metallo-hydrolase Regulator
  RFN65_RS00425 (RFN65_00425) htrA 73093..74295 (+) 1203 WP_021481077.1 serine protease HtrC Regulator
  RFN65_RS00430 (RFN65_00430) - 74315..74437 (+) 123 Protein_81 hypothetical protein -
  RFN65_RS21135 - 74434..74550 (-) 117 Protein_82 peptidase -
  RFN65_RS00435 (RFN65_00435) - 74557..74922 (+) 366 Protein_83 sensor histidine kinase -
  RFN65_RS00440 (RFN65_00440) rocR 74904..76289 (-) 1386 WP_100506033.1 arginine utilization regulatory protein RocR -
  RFN65_RS00445 (RFN65_00445) rocD 76530..77735 (+) 1206 WP_309529952.1 ornithine aminotransferase -

Sequence

Protein


Download         Length: 400 a.a.        Molecular weight: 42756.50 Da        Isoelectric Point: 5.3354

>NTDB_id=800072 RFN65_RS00425 WP_021481077.1 73093..74295(+) (htrA) [Bacillus subtilis strain CP35]
MVDYEREEEHTTPEQPKRSKKGYFLSSLIGVIVGAVLMAFIMPYLSNEGLDTGALDQQQNNNGRESIRTVNVSVNNAVTK
IVSNVSPAVVGVVNIQKSDIWGESGEAGSGSGVIYKKNDHSAYVVTNHHVIEGASQIEISLKDGSRVSADLVGSDQLMDL
AVLRVKSDKIKAVADFGNSDKVKSGEPVIAIGNPLGLEFAGSVTQGVISGTERAIPVDSNGDGQPDWNAEVLQTDAAINP
GNSGGALLNMDGKVIGINSMKIAESAVEGIGLSIPSKLVIPVIEDLERYGKVKRPFLGIEMKSLSDIASYHWDETLKLPK
NVTNGAVVMGVDAFSPAGKAGLKELDVITEFDGYKVNDIVDLRKRLYQKKVGDRVKVKFYRGGKEKSVDIKLSSADQLGS

Nucleotide


Download         Length: 1203 bp        

>NTDB_id=800072 RFN65_RS00425 WP_021481077.1 73093..74295(+) (htrA) [Bacillus subtilis strain CP35]
ATGGTGGATTACGAACGTGAGGAAGAACATACTACTCCTGAACAGCCAAAGAGAAGCAAAAAAGGATATTTTCTTTCGAG
TCTGATTGGCGTGATTGTCGGTGCCGTATTAATGGCGTTTATCATGCCGTACCTTTCAAATGAAGGGCTGGATACGGGCG
CCTTAGATCAGCAGCAAAACAACAACGGCCGGGAATCAATCAGGACGGTGAATGTCAGTGTCAACAATGCCGTCACCAAG
ATTGTCAGCAATGTGTCGCCCGCCGTTGTCGGTGTTGTGAACATCCAAAAATCAGATATTTGGGGAGAGAGCGGCGAGGC
TGGGAGCGGCTCTGGCGTCATCTATAAGAAAAATGACCATTCCGCTTATGTCGTGACCAACCATCATGTCATCGAAGGCG
CTTCCCAAATTGAAATCAGCTTGAAAGACGGCTCACGTGTATCAGCTGATCTTGTCGGCAGCGACCAGCTGATGGACCTT
GCCGTTTTACGGGTGAAAAGCGATAAGATTAAAGCAGTCGCCGATTTCGGAAATTCAGATAAAGTGAAGTCTGGGGAGCC
GGTTATTGCGATCGGGAACCCGTTAGGCCTTGAGTTTGCTGGCTCTGTCACACAAGGCGTCATCTCAGGTACGGAGAGGG
CGATCCCAGTGGATTCAAACGGTGATGGACAGCCTGACTGGAACGCAGAAGTCCTGCAAACAGATGCGGCCATTAATCCT
GGGAACAGCGGCGGTGCTTTGTTAAATATGGATGGGAAGGTCATTGGCATCAATTCAATGAAAATTGCCGAGTCGGCGGT
TGAAGGGATTGGCCTGTCGATTCCATCTAAGCTCGTGATCCCTGTGATAGAGGATTTAGAGAGATACGGAAAGGTCAAAC
GCCCGTTCCTTGGGATTGAGATGAAATCGCTAAGCGACATCGCAAGCTATCATTGGGATGAAACATTAAAGCTTCCTAAG
AATGTTACCAATGGAGCGGTTGTGATGGGTGTAGACGCCTTTTCACCTGCCGGAAAAGCGGGGCTGAAGGAACTGGATGT
CATCACGGAATTTGACGGATACAAAGTAAATGATATTGTTGACCTGCGAAAACGGCTTTATCAGAAAAAAGTCGGTGACC
GGGTGAAGGTGAAGTTTTACCGCGGCGGAAAAGAAAAATCTGTAGACATCAAGCTGTCCTCCGCAGACCAATTAGGCAGT
TAA

Domains

Predicted by InterProScan.

PDZ domain (PDZ_2)

(295-392)

Peptidase family M50 (Peptidase_M50)

(185-395)

Trypsin-like peptidase domain (Trypsin_2)

(110-257)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  htrA Streptococcus mutans UA159

43.829

99.25

0.435
  htrA Streptococcus gordonii str. Challis substr. CH1

41.542

100

0.417
  htrA Streptococcus mitis NCTC 12261

43.005

96.5

0.415
  htrA Streptococcus pneumoniae TIGR4

45.152

82.5

0.373
  htrA Streptococcus pneumoniae D39

45.152

82.5

0.373
  htrA Streptococcus pneumoniae Rx1

45.152

82.5

0.373
  htrA Streptococcus pneumoniae R6

45.152

82.5

0.373