Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   RAM33_RS02345 Genome accession   NZ_CP132372
Coordinates   413051..414055 (+) Length   334 a.a.
NCBI ID   WP_001005768.1    Uniprot ID   P66757
Organism   Staphylococcus aureus strain UNC_SA3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 408051..419055
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM33_RS02310 (RAM33_02305) mreD 408593..409123 (+) 531 WP_001259925.1 rod shape-determining protein MreD -
  RAM33_RS02315 (RAM33_02310) rplU 409359..409667 (+) 309 WP_000457386.1 50S ribosomal protein L21 -
  RAM33_RS02320 (RAM33_02315) - 409673..409993 (+) 321 WP_000633692.1 ribosomal-processing cysteine protease Prp -
  RAM33_RS02325 (RAM33_02320) rpmA 410005..410289 (+) 285 WP_000916187.1 50S ribosomal protein L27 -
  RAM33_RS02330 (RAM33_02325) obgE 410643..411935 (+) 1293 WP_000496100.1 GTPase ObgE -
  RAM33_RS02335 (RAM33_02330) - 411948..412403 (+) 456 WP_000368902.1 ACT domain-containing protein -
  RAM33_RS02340 (RAM33_02335) ruvA 412417..413019 (+) 603 WP_000271550.1 Holliday junction branch migration protein RuvA Machinery gene
  RAM33_RS02345 (RAM33_02340) ruvB 413051..414055 (+) 1005 WP_001005768.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  RAM33_RS02350 (RAM33_02345) queA 414057..415082 (+) 1026 WP_001019178.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  RAM33_RS02355 (RAM33_02350) tgt 415105..416244 (+) 1140 WP_001112045.1 tRNA guanosine(34) transglycosylase Tgt -
  RAM33_RS02360 (RAM33_02355) yajC 416263..416523 (+) 261 WP_001160682.1 preprotein translocase subunit YajC -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 37645.94 Da        Isoelectric Point: 5.1876

>NTDB_id=796604 RAM33_RS02345 WP_001005768.1 413051..414055(+) (ruvB) [Staphylococcus aureus strain UNC_SA3]
MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIKAAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVN
IRTVSGPSLERPGDLAAILSGLQPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTPRVANRLLKRVRDFQQVNEDEQI
YIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNGGPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATP
LAYEHFAKSNEERG

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=796604 RAM33_RS02345 WP_001005768.1 413051..414055(+) (ruvB) [Staphylococcus aureus strain UNC_SA3]
ATGAATGAGCGTATGGTTGATCAATCAATGCATAGTGAAGAAACTGATTTCGAATTGTCGCTTAGACCTACGAGATTACG
ACAATATATTGGTCAAAATTCAATAAAAAGTAATTTAGAAGTATTTATTAAAGCGGCTAAACTTCGTCATGAACCATTAG
ATCATGTATTGCTTTTTGGCCCCCCTGGATTAGGTAAGACAACATTATCTAATATCATTGCCAATGAAATGGAAGTTAAT
ATACGTACAGTATCAGGGCCTTCATTAGAAAGACCTGGTGATTTGGCTGCAATTTTATCAGGACTTCAACCTGGAGATGT
ATTGTTTATTGATGAAATACACAGACTGAGTAGTGTTGTTGAAGAAGTGTTATACCCTGCAATGGAAGATTTCTTTTTAG
ATATTATCATTGGTAAAGGCGATGAGGCTAGAAGTATCCGTATCGACTTACCTCCATTCACTTTGGTAGGTGCAACAACG
CGAGCTGGCAGCTTAACAGGTCCACTAAGGGATCGATTTGGTGTGCACTTAAGATTAGAATATTATAATGAATCAGATTT
AAAAGAAATCATTATTAGAACAGCTGAGGTTTTAGGCACAGGTATTGATGAAGAAAGTGCCATTGAACTTGCTAAACGTT
CTAGAGGGACTCCAAGAGTAGCAAATCGACTATTGAAGCGGGTAAGAGACTTCCAGCAAGTGAATGAAGATGAACAAATA
TACATTGAAACAACGAAGCACGCATTAGGTTTACTTCAAGTTGATCAACACGGACTAGATTACATTGATCATAAAATGAT
GAACTGTATTATTAAGCAGTATAATGGTGGACCTGTTGGTTTAGATACGATTGCCGTAACAATTGGTGAAGAACGTATTA
CAATTGAGGACGTTTATGAGCCATTTCTTATTCAGAAAGGCTTTTTAGAACGTACGCCACGTGGCAGAAAAGCAACACCA
TTAGCTTATGAACATTTTGCAAAGTCGAATGAGGAGAGAGGATAA

Domains

Predicted by InterProScan.

RuvB AAA lid domain (AAA_lid_4)

(182-255)

RuvB C-terminal winged helix domain (WHD_RuvB)

(257-326)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(21-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P66757

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

65.951

97.605

0.644
  ruvB Streptococcus pneumoniae TIGR4

58.182

98.802

0.575
  ruvB Streptococcus pneumoniae R6

57.879

98.802

0.572
  ruvB Streptococcus pneumoniae D39

57.879

98.802

0.572
  ruvB Helicobacter pylori 26695

56.481

97.006

0.548
  ruvB Synechocystis sp. PCC 6803

53.75

95.808

0.515