Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PWO49_RS13725 Genome accession   NZ_CP118916
Coordinates   3050192..3051871 (+) Length   559 a.a.
NCBI ID   WP_210797572.1    Uniprot ID   -
Organism   Pseudoalteromonas ostreae strain hOe-124     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3045192..3056871
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWO49_RS13705 - 3045649..3046692 (+) 1044 WP_228732993.1 O-antigen ligase family protein -
  PWO49_RS13710 pilC 3046695..3047924 (+) 1230 WP_210797575.1 type II secretion system F family protein Machinery gene
  PWO49_RS13715 pilD 3048519..3049418 (+) 900 WP_210797574.1 A24 family peptidase Machinery gene
  PWO49_RS13720 coaE 3049420..3050055 (+) 636 WP_210797573.1 dephospho-CoA kinase -
  PWO49_RS13725 pilB 3050192..3051871 (+) 1680 WP_210797572.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PWO49_RS13730 yacG 3051939..3052169 (+) 231 WP_210797571.1 DNA gyrase inhibitor YacG -
  PWO49_RS13735 - 3052268..3053440 (-) 1173 WP_210797664.1 divalent metal cation transporter -
  PWO49_RS13740 raiA 3053634..3053990 (-) 357 WP_145235189.1 ribosome-associated translation inhibitor RaiA -
  PWO49_RS13745 - 3054174..3054485 (-) 312 WP_210797570.1 pyrimidine/purine nucleoside phosphorylase -
  PWO49_RS13750 - 3054777..3055115 (-) 339 WP_275074494.1 hypothetical protein -
  PWO49_RS13755 - 3055206..3056303 (-) 1098 WP_275074495.1 ISAs1 family transposase -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62477.87 Da        Isoelectric Point: 5.6071

>NTDB_id=796320 PWO49_RS13725 WP_210797572.1 3050192..3051871(+) (pilB) [Pseudoalteromonas ostreae strain hOe-124]
MNINSPLLRKFITLGRIDAETVKLKQREYASTAELIAKCSGIDGHELAEQCIDLFRVPYFDIREFDVELIPKELVKEKLI
KDHHILPLVKKGRKLYIAASDPTDYGAFENFEFSTGLACEVVVVDYRQLEVKIDQLLDASGSLHLSDEEFKEFSDLDTES
PREQQNNGEDEKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRVRFRIDGILHEMASPPNSLATRLSARIKVMARLD
IAEKRKPQDGRIKLKITERKSIDFRVSTLPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKKLYMDALQQPQGMILVTGP
TGSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGINQVQINPRAEMTFANALKAFLRQDPDVVMVGEIRDLETAEISI
KAAQTGHLVLSTLHTNSAPETLTRLLNMGVPAYNVASSISLIIAQRLARRLCPKCKTPETLPSEELQRQGFSPEQISELT
LFVPKGCDSCTDGYKGRVGIYEVMQITPEIAQIIMRGGNSLEIAEVSLKSGFNNLRLSGLRKAAAGMTSLAEINRVTSF

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=796320 PWO49_RS13725 WP_210797572.1 3050192..3051871(+) (pilB) [Pseudoalteromonas ostreae strain hOe-124]
ATGAATATTAATTCACCATTACTTAGAAAGTTTATTACGCTTGGCCGTATTGACGCTGAAACGGTTAAATTAAAGCAACG
TGAGTATGCCTCAACCGCAGAGCTAATAGCTAAATGCAGCGGTATTGACGGCCATGAGCTTGCCGAGCAATGCATTGACT
TATTCCGAGTGCCTTATTTTGATATACGTGAGTTTGATGTCGAGCTGATCCCAAAAGAGCTTGTTAAAGAAAAGCTGATC
AAAGATCACCATATTCTACCTTTAGTTAAAAAAGGCCGTAAACTTTATATTGCCGCGTCTGATCCTACCGATTACGGTGC
TTTTGAAAACTTTGAATTTAGTACCGGACTTGCCTGTGAGGTAGTCGTGGTTGATTATCGCCAGCTTGAAGTCAAAATAG
ATCAACTACTTGATGCCTCAGGCAGTTTGCATTTAAGTGATGAAGAATTTAAAGAGTTTAGCGATCTAGATACCGAAAGT
CCTCGAGAACAACAAAATAACGGTGAAGACGAAAAAGACGATGCCCCAATTATTGTCTACATCAATAAAATTTTGATGGA
TGCAATAAAAAAAGGCGCGTCGGATCTACACTTTGAACCTTACGAACATAAATACCGCGTACGTTTTCGTATCGATGGTA
TTTTGCATGAAATGGCCAGCCCGCCGAACAGCTTAGCCACGCGCTTATCTGCGCGTATAAAAGTTATGGCGCGCTTAGAT
ATTGCCGAAAAACGAAAACCACAAGATGGCCGTATCAAACTAAAAATTACCGAACGTAAAAGTATCGATTTCCGGGTAAG
TACGCTGCCGACCTTATGGGGCGAGAAAATAGTAATGCGTATCCTTGATTCCTCCAGTGCCATGCTGGGCATTGATGTAC
TGGGTTATGAACCTGAGCAGAAAAAGCTCTATATGGATGCTCTGCAACAACCGCAAGGCATGATTTTAGTTACTGGCCCT
ACCGGCTCGGGTAAAACAGTATCACTTTACACTGGTTTAAATATTCTTAACCAACCAGAGCGTAATATCAGCACTGCAGA
AGATCCGGTTGAAATTAACCTTGAAGGTATCAACCAAGTACAAATTAACCCGCGCGCAGAAATGACCTTTGCTAATGCGT
TAAAAGCGTTTTTACGTCAAGATCCTGATGTAGTAATGGTAGGTGAGATCCGTGATCTTGAAACCGCTGAAATTTCTATC
AAAGCGGCGCAAACTGGTCACTTAGTATTATCGACATTACATACTAACTCTGCACCTGAAACCCTAACCCGTTTACTTAA
TATGGGTGTACCTGCTTATAACGTGGCCAGCTCGATTAGCTTGATCATCGCACAGCGTTTAGCCCGTCGTTTATGCCCTA
AATGTAAAACACCTGAAACCTTACCTAGTGAAGAGTTACAACGCCAAGGTTTTAGCCCTGAACAAATAAGTGAACTCACT
TTATTTGTCCCCAAAGGCTGCGATAGTTGTACAGATGGCTATAAAGGCCGGGTAGGTATTTATGAAGTAATGCAAATTAC
CCCTGAAATTGCACAGATCATTATGCGTGGCGGTAACTCACTAGAAATTGCTGAAGTATCGCTAAAGTCAGGCTTTAATA
ACTTGCGTTTATCAGGCCTCAGAAAAGCTGCCGCCGGTATGACTTCATTAGCCGAGATAAACCGAGTAACTAGTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(183-450)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.583

92.129

0.558
  pilB Acinetobacter baylyi ADP1

59.528

91.055

0.542
  pilB Legionella pneumophila strain ERS1305867

53.519

96.601

0.517
  pilB Vibrio cholerae strain A1552

54.221

95.349

0.517
  pilB Vibrio campbellii strain DS40M4

52.052

95.886

0.499
  pilB Vibrio parahaemolyticus RIMD 2210633

53.359

93.202

0.497
  pilF Neisseria gonorrhoeae MS11

49.904

93.202

0.465